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FunGene: 08-OCT-2024
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus JSNZ
- locus tag: JSNZ_002400
- pan locus tag?: SAUPAN005967000
- symbol: bioD
- pan gene symbol?: bioD
- synonym:
- product: dethiobiotin synthase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: JSNZ_002400
- symbol: bioD
- product: dethiobiotin synthase
- replicon: chromosome
- strand: -
- coordinates: 2397151..2397837
- length: 687
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID:
- RefSeq:
- BioCyc:
- MicrobesOnline:
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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661ATGAGGATTTTTATTACAAGTACGAATACTGATGTAGGCAAAACCTATGTTACAAAGCAT
TTATACCATGCTTTGAAAACACGTGGTCATCGAGTTTGTATTTTTAAACCATTTCAAACT
GAGGAACGCCAAGACGGTACGTTTCCAGATTTAGAAGTATTTAAAAATGAATGTGATTTA
AGCTATGACATAACGTCACTTTATACTTTTAAGCAACCTGTATCACCACACCTTGCATTT
AAAATGACAGATCAAATTTTTCTAAATAAGCAGCGTGTATTAGATAAGGTAAAAGTTTTA
GATAAGGAATTTGATTTTATCTTAATTGAGGGTGCTGGGGGAATTGCCGTACCAATATAT
GAAGGTACAGATGATTTCTACATGACTAAAGATCTAATCAATGATTGTGCAGATTGTGTC
ATCAGTGTGTTGCCATCAAAATTAGGTGCTATTAGCGATGCCATTGTTCACCAAGATTAT
GTTAATCAGAATGTATCGGCGAGTAATTTTTTAATAATGAATCGCTATACAGACAGCTAT
ATTGAAAAAGACAATCAAATTACGATTGGAAAATTAACAAATAAAACAGTCTATACATTT
GAAGAACATGCCACGTATGAAAATTTCTCAGAAGCATTTTTAAAACAATTAATAGGAGTT
AAAAATGAATTACACACAACAACTTAA60
120
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: JSNZ_002400
- symbol: BioD
- description: dethiobiotin synthase
- length: 228
- theoretical pI: 5.59134
- theoretical MW: 26140.5
- GRAVY: -0.260965
⊟Function[edit | edit source]
- reaction: EC 6.3.3.3? ExPASyDethiobiotin synthase ATP + 7,8-diaminononanoate + CO2 = ADP + phosphate + dethiobiotin
- TIGRFAM: Biosynthesis of cofactors, prosthetic groups, and carriers Biotin dethiobiotin synthase (TIGR00347; EC 6.3.3.3; HMM-score: 103.8)and 5 moreCellular processes Cell division septum site-determining protein MinD (TIGR01968; HMM-score: 30.7)Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin cobyric acid synthase CobQ (TIGR00313; EC 6.3.5.10; HMM-score: 20.6)Biosynthesis of cofactors, prosthetic groups, and carriers Molybdopterin molybdopterin-guanine dinucleotide biosynthesis protein B (TIGR00176; HMM-score: 13.7)cell division ATPase MinD (TIGR01969; HMM-score: 12.4)Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides cellulose synthase operon protein YhjQ (TIGR03371; HMM-score: 11.6)
- TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
- PFAM: P-loop_NTPase (CL0023) AAA_26; AAA domain (PF13500; HMM-score: 82.5)and 4 moreCbiA; CobQ/CobB/MinD/ParA nucleotide binding domain (PF01656; HMM-score: 26.7)DUF1611; Domain of unknown function (DUF1611_C) P-loop domain (PF07755; HMM-score: 20.1)MobB; Molybdopterin guanine dinucleotide synthesis protein B (PF03205; HMM-score: 16.3)NADP_Rossmann (CL0063) Rimk_N; RimK PreATP-grasp domain (PF18030; HMM-score: 12.9)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 9.97
- Cytoplasmic Membrane Score: 0
- Cellwall Score: 0.01
- Extracellular Score: 0.02
- Internal Helices: 0
- DeepLocPro: Cytoplasmic
- Cytoplasmic Score: 0.9698
- Cytoplasmic Membrane Score: 0.0177
- Cell wall & surface Score: 0.0001
- Extracellular Score: 0.0124
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.00819
- TAT(Tat/SPI): 0.000251
- LIPO(Sec/SPII): 0.001262
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
- GI:
- RefSeq:
- UniProt:
⊟Protein sequence[edit | edit source]
- MRIFITSTNTDVGKTYVTKHLYHALKTRGHRVCIFKPFQTEERQDGTFPDLEVFKNECDLSYDITSLYTFKQPVSPHLAFKMTDQIFLNKQRVLDKVKVLDKEFDFILIEGAGGIAVPIYEGTDDFYMTKDLINDCADCVISVLPSKLGAISDAIVHQDYVNQNVSASNFLIMNRYTDSYIEKDNQITIGKLTNKTVYTFEEHATYENFSEAFLKQLIGVKNELHTTT
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- Operon-mapper [1] : JSNZ_002395 < JSNZ_002396 < JSNZ_002397 < bioB < bioA < bioD < JSNZ_002401
⊟Regulation[edit | edit source]
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You can add further information about the gene and protein here. [edit]
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
Bioinformatics: 2018, 34(23);4118-4120
[PubMed:29931111] [WorldCat.org] [DOI] (I p) - ↑ Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
Curr Res Microb Sci: 2025, 9;100489
[PubMed:41146725] [WorldCat.org] [DOI] (I e)