From AureoWiki
Jump to navigation Jump to search

FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_002397
  • pan locus tag?: SAUPAN005964000
  • symbol: JSNZ_002397
  • pan gene symbol?: bioF
  • synonym:
  • product: aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_002397
  • symbol: JSNZ_002397
  • product: aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme
  • replicon: chromosome
  • strand: -
  • coordinates: 2393713..2394828
  • length: 1116
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGATGTTTCAAGAGCAAATTGATTTATTACAACAGAAAGGGTTATATCGGTCGCTTAAA
    TCGGTAGGGTATATAGATAGACAGTATATTGAAGTAGAAAATAAGAGATGTACGAACTAT
    ACATCTAATGATTATTTAGGATTAGGTCAAATAGCGTTTGATAAGGATGATTTCGAAAGA
    TTTATGCGGAAGTATAGTTATCACTTATCAAGTTCAAGATTAATTAGTGGAAGTTCGACA
    GCTTATGAAGAAATTGAAACAATGTTAGCAGGTTGGCTCGGATATAGTGCATGTACTATC
    TTAAATAGTGGTTATGATGCTAATTTGGCGTTGTTTAATATTTTCAAAAATACAAATTGT
    GTCGTGTTTTCAGATCAAGAAAATCATGCGAGTATTATTGACGGTATTAAGTTAAGTGGT
    TTAGAAAAAGTGATATATAAGCATTTAGATATTGCTGATTTAGAAAAAAGGTTAGCGGAA
    TACCCTAATCAAAATATACCAAAAATAATCATATCTGATAGTGTATTTTCAACGAATGGC
    GACGTTGTGGATATTGGTCAATTAGTCTCATTAAAGCATAAATATAACGCAACACTGATA
    CTCGATGTTTCACATAGTTTTGGAATAGAGAATTACTCGAATTATCAAGGTGTAGATATA
    CTCACTTCTAGTTTATCTAAAGCATGTGGTGCATACGGGGGTGTGATTTTAAGTTCAAAT
    GATGTGAAGGATATGTTAATTAATCACGGTAGACCACTCATCTACTCAAGTAGTTTGCCA
    ATTTATAATTTGTATTTTATAAAAAGAAACATTGAAAAGTTAATAAATGCTGATGATAGA
    CGCACTAAATTAAATAGTTTGAGTAAATATTTTAACCAAAAGTTAAAAGCACTCAATGTT
    AATTATAATAGTTCAAACTCGCCGATTAAGTTTATTGAGTTTGATGACATAGAAGCGGCT
    GAGAACATTCATCAAACATTATTAAAGCATCATGTGTTTACAAGTTATTTAAGGTATCCA
    ACTGTGACTAAGCCAATGTTAAGAATATCATTGTCGTATTTTCATACTGAACAAGATATA
    GATAGGTTGTTTGAAATTTTGCATCAAGAAGATTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1116

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_002397
  • symbol: JSNZ_002397
  • description: aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme
  • length: 371
  • theoretical pI: 6.4387
  • theoretical MW: 42506.9
  • GRAVY: -0.283558

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Biotin 8-amino-7-oxononanoate synthase (TIGR00858; EC 2.3.1.47; HMM-score: 270.5)
    putative pyridoxal phosphate-dependent acyltransferase (TIGR01825; EC 2.3.1.-; HMM-score: 218.8)
    and 9 more
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin 5-aminolevulinic acid synthase (TIGR01821; EC 2.3.1.37; HMM-score: 184.3)
    Metabolism Energy metabolism Amino acids and amines glycine C-acetyltransferase (TIGR01822; EC 2.3.1.29; HMM-score: 183.1)
    Unknown function Enzymes of unknown specificity cysteine desulfurase family protein (TIGR01977; HMM-score: 24.3)
    UDP-4-amino-4,6-dideoxy-N-acetyl-beta-L-altrosamine transaminase (TIGR03588; EC 2.6.1.92; HMM-score: 23.5)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other cysteine desulfurase, SufS family (TIGR01979; HMM-score: 21.7)
    Metabolism Amino acid biosynthesis Aspartate family O-succinylhomoserine sulfhydrylase (TIGR01325; EC 4.2.99.-; HMM-score: 14.2)
    2-aminoethylphosphonate aminotransferase (TIGR03301; EC 2.6.1.-; HMM-score: 13.7)
    Metabolism Amino acid biosynthesis Aspartate family O-acetylhomoserine aminocarboxypropyltransferase/cysteine synthase (TIGR01326; HMM-score: 13.5)
    Metabolism Amino acid biosynthesis Serine family O-acetylhomoserine aminocarboxypropyltransferase/cysteine synthase (TIGR01326; HMM-score: 13.5)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    PLP_aminotran (CL0061) Aminotran_1_2; Aminotransferase class I and II (PF00155; HMM-score: 133.5)
    and 5 more
    Aminotran_5; Aminotransferase class-V (PF00266; HMM-score: 31.2)
    Beta_elim_lyase; Beta-eliminating lyase (PF01212; HMM-score: 24.1)
    Cys_Met_Meta_PP; Cys/Met metabolism PLP-dependent enzyme (PF01053; HMM-score: 19)
    DegT_DnrJ_EryC1; DegT/DnrJ/EryC1/StrS aminotransferase family (PF01041; HMM-score: 13.4)
    TIM_barrel (CL0036) DAHP_synth_1; DAHP synthetase I family (PF00793; HMM-score: 13.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9254
    • Cytoplasmic Membrane Score: 0.0097
    • Cell wall & surface Score: 0.0001
    • Extracellular Score: 0.0648
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.005086
    • TAT(Tat/SPI): 0.000653
    • LIPO(Sec/SPII): 0.001323
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MMFQEQIDLLQQKGLYRSLKSVGYIDRQYIEVENKRCTNYTSNDYLGLGQIAFDKDDFERFMRKYSYHLSSSRLISGSSTAYEEIETMLAGWLGYSACTILNSGYDANLALFNIFKNTNCVVFSDQENHASIIDGIKLSGLEKVIYKHLDIADLEKRLAEYPNQNIPKIIISDSVFSTNGDVVDIGQLVSLKHKYNATLILDVSHSFGIENYSNYQGVDILTSSLSKACGAYGGVILSSNDVKDMLINHGRPLIYSSSLPIYNLYFIKRNIEKLINADDRRTKLNSLSKYFNQKLKALNVNYNSSNSPIKFIEFDDIEAAENIHQTLLKHHVFTSYLRYPTVTKPMLRISLSYFHTEQDIDRLFEILHQED

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: BirA* (repression) regulon
    BirA*(TF)important in Biotin biosynthesis;  regulation predicted or transferred from N315 and NCTC 8325  [2]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)
  2. ↑ Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=JSNZ_002397&oldid=108038"