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FunGene: 08-OCT-2024
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus JSNZ
- locus tag: JSNZ_001099
- pan locus tag?: SAUPAN003367000
- symbol: srtB
- pan gene symbol?: srtB
- synonym:
- product: class B sortase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: JSNZ_001099
- symbol: srtB
- product: class B sortase
- replicon: chromosome
- strand: +
- coordinates: 1103387..1104121
- length: 735
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID:
- RefSeq:
- BioCyc:
- MicrobesOnline:
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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721ATGAGAATGAAGCGATTTTTAACTATTGTACAAATTTTATTGGTTGTAATTATTATCATT
TTTGGTTACAAAATTGTTCAAACATATATTGAAGACAAGCAAGAACGCGCAAATTATGAG
AAATTACAACAAAAATTTCAAATGTTGATGAGCAAACATCAAGCACATGTGAGACCACAA
TTTGAATCACTTGAAAAAATAAATAAAGACATTGTTGGATGGATAAAATTATCAGGAACA
TCATTAAATTATCCAGTACTACAAGGTAAGACAAATCACGATTATTTAAATTTAGATTTT
GAGCGAGAACATCGACGTAAAGGTAGTATTTTTATGGATTTTAGAAATGAATTGAAGAAT
TTAAATCATAATACTATTTTATACGGGCACCATGTCGGTGATAATACGATGTTTGATGTG
TTAGAGGATTATTTAAAGCAATCGTTTTATGAAAAACACAAGATAATTGAATTTGACAAT
AAATATGGTAAATATCAATTGCAAGTATTTAGTGCATATAAAACTACTACTAAAGATAAT
TACATACGTACAGAGTTTGAAAATGATCAAGATTATCAACAATTTTTAAATGAGACAAAA
CGTAAATCTGTAATTAACTCAGATGTTAATGTAACGGTAAAAGATAAAATAATGACTTTA
TCAACGTGCGAAGATGCATATAGTGAAACAACGAAAAGAATTGTTGTTGTCGCAAAAATA
ATTAAGGTAAGTTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: JSNZ_001099
- symbol: SrtB
- description: class B sortase
- length: 244
- theoretical pI: 9.51751
- theoretical MW: 29090.2
- GRAVY: -0.558607
⊟Function[edit | edit source]
- reaction: EC 3.4.22.71? ExPASySortase B The enzyme catalyzes a cell wall sorting reaction in which a surface protein with a sorting signal containing a NXTN motif is cleaved. The resulting threonine carboxyl end of the protein is covalently attached to a pentaglycine cross-bridge of peptidoglycan
- TIGRFAM: Cell envelope Other sortase, SrtB family (TIGR03064; EC 3.4.22.71; HMM-score: 302.4)Protein fate Protein and peptide secretion and trafficking sortase, SrtB family (TIGR03064; EC 3.4.22.71; HMM-score: 302.4)and 2 moreCell envelope Other sortase (TIGR01076; EC 3.4.22.-; HMM-score: 12.9)Protein fate Protein and peptide secretion and trafficking sortase (TIGR01076; EC 3.4.22.-; HMM-score: 12.9)
- TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
- PFAM: no clan defined Sortase; Sortase domain (PF04203; HMM-score: 66.8)and 6 moreDeath (CL0041) Death_Lrrd1; Lrrd1 death domain (PF24978; HMM-score: 16)no clan defined Sigma_reg_N; Sigma factor regulator N-terminal (PF13800; HMM-score: 13.7)TPR (CL0020) TPR_21; Tetratricopeptide repeat-like domain (PF09976; HMM-score: 13.2)no clan defined HJURP_C; Holliday junction regulator protein family C-terminal repeat (PF12347; HMM-score: 11.4)C_Lectin (CL0056) Ly49; Ly49-like protein, N-terminal region (PF08391; HMM-score: 8.7)no clan defined Arc_PepC_II; Archaeal Peptidase A24 C-terminus Type II (PF06847; HMM-score: 8.5)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: unknown (no significant prediction)
- Cytoplasmic Score: 2.5
- Cytoplasmic Membrane Score: 2.5
- Cellwall Score: 2.5
- Extracellular Score: 2.5
- Internal Helix: 1
- DeepLocPro: Cytoplasmic Membrane
- Cytoplasmic Score: 0.0002
- Cytoplasmic Membrane Score: 0.8344
- Cell wall & surface Score: 0.0092
- Extracellular Score: 0.1563
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.307895
- TAT(Tat/SPI): 0.000596
- LIPO(Sec/SPII): 0.021595
- predicted transmembrane helices (TMHMM): 1
⊟Accession numbers[edit | edit source]
- GI:
- RefSeq:
- UniProt:
⊟Protein sequence[edit | edit source]
- MRMKRFLTIVQILLVVIIIIFGYKIVQTYIEDKQERANYEKLQQKFQMLMSKHQAHVRPQFESLEKINKDIVGWIKLSGTSLNYPVLQGKTNHDYLNLDFEREHRRKGSIFMDFRNELKNLNHNTILYGHHVGDNTMFDVLEDYLKQSFYEKHKIIEFDNKYGKYQLQVFSAYKTTTKDNYIRTEFENDQDYQQFLNETKRKSVINSDVNVTVKDKIMTLSTCEDAYSETTKRIVVVAKIIKVS
⊟Experimental data[edit | edit source]
- experimentally validated: data available for NCTC8325
- protein localization:
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You can add further information about the gene and protein here. [edit]
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
Bioinformatics: 2018, 34(23);4118-4120
[PubMed:29931111] [WorldCat.org] [DOI] (I p) - ↑ Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
Curr Res Microb Sci: 2025, 9;100489
[PubMed:41146725] [WorldCat.org] [DOI] (I e)