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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_001095
  • pan locus tag?: SAUPAN003360000
  • symbol: isdC
  • pan gene symbol?: isdC
  • synonym:
  • product: heme uptake protein IsdC

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_001095
  • symbol: isdC
  • product: heme uptake protein IsdC
  • replicon: chromosome
  • strand: +
  • coordinates: 1099713..1100396
  • length: 684
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    TTGAAAAATATTTTAAAAGTTTTTAATACAACGATTTTAGCGTTAATTATCATCATCGCG
    ACATTCAGTAATTCTGCAAATGCCGCAGATAGCGGTACTTTGAATTATGAGGTTTACAAA
    TACAATACCAATGACACGTCAATTGCTAATGACTATTTTAATAAACCGGCAAAGTACATT
    AAGAAAAATGGTAAATTGTATGTTCAAATAACTGTCAACCACAGTCATTGGATTACTGGA
    ATGAGTATCGAAGGACATAAAGAAAATATTATTAGTAAAAACACTGCCAAAGATGAACGC
    ACTTCTGAATTTGAAGTAAGTAAGTTGAACGGTAAAATAGATGGAAAAATTGACGTTTAT
    ATCGATGAAAAAGTAAATGGTAAGCCATTCAAATATGACCATCATTACAACATTACATAT
    AAATTTAATGGACCAACTGATGTAGCAGGTGCTAATGCACCAGGTAAAGATGATAAAAAT
    TCTGCTTCAGGTAGTGACAAAGGATCTGATGGAACGACTACTGGTCAAAGTGAATCTAAT
    AGTTCGAATAAAGACAAAGTAGAAAATCCACAAACAAATGCTGGTACACCTGCATATATA
    TATGCAATACCAGTTGCATCCTTAGCATTATTAATCGCAATCACATTGTTTGTTAGAAAA
    AAATCTAAAGGCAATGTGGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    684

⊟Protein[edit | edit source]

Protein Data Bank: 2O6P

⊟General[edit | edit source]

  • locus tag: JSNZ_001095
  • symbol: IsdC
  • description: heme uptake protein IsdC
  • length: 227
  • theoretical pI: 9.42324
  • theoretical MW: 24854.6
  • GRAVY: -0.564317

⊟Function[edit | edit source]

  • TIGRFAM:
    heme uptake protein IsdC (TIGR03656; HMM-score: 320.3)
    and 2 more
    sortase B signal domain, NPQTN class (TIGR03068; HMM-score: 87.3)
    heme uptake protein IsdB (TIGR03657; HMM-score: 14.1)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    E-set (CL0159) NEAT; Iron Transport-associated domain (PF05031; HMM-score: 102.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 3.33
    • Cellwall Score: 3.33
    • Extracellular Score: 3.33
    • Internal Helices: 2
  • DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.0001
    • Cytoplasmic Membrane Score: 0.5454
    • Cell wall & surface Score: 0.215
    • Extracellular Score: 0.2396
  • LocateP:
  • SignalP: Signal peptide SP(Sec/SPI) length 28 aa
    • SP(Sec/SPI): 0.998468
    • TAT(Tat/SPI): 0.000645
    • LIPO(Sec/SPII): 0.000521
    • Cleavage Site: CS pos: 28-29. ANA-AD. Pr: 0.9892
  • predicted transmembrane helices (TMHMM): 2

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MKNILKVFNTTILALIIIIATFSNSANAADSGTLNYEVYKYNTNDTSIANDYFNKPAKYIKKNGKLYVQITVNHSHWITGMSIEGHKENIISKNTAKDERTSEFEVSKLNGKIDGKIDVYIDEKVNGKPFKYDHHYNITYKFNGPTDVAGANAPGKDDKNSASGSDKGSDGTTTGQSESNSSNKDKVENPQTNAGTPAYIYAIPVASLALLIAITLFVRKKSKGNVE

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: Fur* (repression) regulon
    Fur*(TF)important in Iron homeostasis;  regulation predicted or transferred from N315 and NCTC 8325  [2]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)
  2. ↑ Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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