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FunGene: 08-OCT-2024
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus JSNZ
- locus tag: JSNZ_001097
- pan locus tag?: SAUPAN003363000
- symbol: isdE
- pan gene symbol?: isdE
- synonym:
- product: heme ABC transporter substrate-binding protein IsdE
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: JSNZ_001097
- symbol: isdE
- product: heme ABC transporter substrate-binding protein IsdE
- replicon: chromosome
- strand: +
- coordinates: 1101469..1102347
- length: 879
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID:
- RefSeq:
- BioCyc:
- MicrobesOnline:
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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841TTGAGAATCATAAAGTATTTAACCATTTTAGTGATAAGCGTCGTTATCTTAACCAGCTGT
CAATCTTCCAGTTCTCAAGAATCAACTAAATCCGGCGAATTCAGAATCGTACCAACAACT
GTTGCATTGACAATGACATTGGACAAATTGGATTTACCAATTGTCGGCAAACCCACGTCA
TATAAGACATTGCCTAATCGTTATAAAGATGTACCGGAAATTGGTCAACCAATGGAGCCG
AATGTTGAAGCTGTTAAAAAGTTAAAACCAACACATGTTTTGAGTGTGTCAACGATTAAA
GATGAAATGCAACCATTTTACAAACAATTAAATATGAAAGGCTACTTTTATGATTTTGAT
AGTTTAAAAGGGATGCAAAAGTCGATTACACAATTAGGTGATCAATTTAATCGTAAAGCA
CAAGCAAAAGAATTAAATGACCATTTAAATTCTGTAAAGCAAAAAATTGAAAATAAAGCA
GCTAAACAAAAGAAACATCCCAAAGTATTAATATTAATGGGTGTACCGGGTAGCTATTTA
GTAGCAACTGATAAATCATATATTGGTGATTTAGTTAAAATAACAGGTGGAGAAAATGTT
ATTAAAGTGAAAGATCGTCAATATATTTCGTCTAATACTGAAAATTTGTTGAATATCAAT
CCAGATATTATTTTAAGATTACCACACGGAATGCCTGAAGAAGTTAAGAAAATGTTTCAA
AAAGAATTTAAACAAAATGATATTTGGAAACATTTTAAAGCTGTGAAAAATAATCATGTT
TATGACTTAGAGGAAGTGCCATTCGGTATTACAGCAAATGTTGATGCTGATAAGGCAATG
ACTCAATTATATGATTTATTTTATAAGGATAAAAAATAG60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: JSNZ_001097
- symbol: IsdE
- description: heme ABC transporter substrate-binding protein IsdE
- length: 292
- theoretical pI: 9.91166
- theoretical MW: 33300.5
- GRAVY: -0.449315
⊟Function[edit | edit source]
- TIGRFAM: heme ABC transporter, heme-binding protein isdE (TIGR03659; HMM-score: 409.7)and 3 moreputative ABC transporter PGF-CTERM-modified substrate-binding protein (TIGR04281; HMM-score: 41.3)Cell envelope Other uncharacterized lipoprotein (TIGR02722; HMM-score: 13.1)Protein fate Protein and peptide secretion and trafficking twin arginine-targeting protein translocase TatB (TIGR01410; HMM-score: 7.5)
- TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
- PFAM: Chelatase (CL0043) Peripla_BP_2; Periplasmic binding protein (PF01497; HMM-score: 142.6)and 6 moreno clan defined Muted; Organelle biogenesis, Muted-like protein (PF14942; HMM-score: 18.2)HAD (CL0137) NT5C; 5' nucleotidase, deoxy (Pyrimidine), cytosolic type C protein (NT5C) (PF06941; HMM-score: 15.9)no clan defined DUF2098; Uncharacterized protein conserved in archaea (DUF2098) (PF09871; HMM-score: 15)GDH2_N; NAD-dependent glutamate dehydrogenase, N-terminal domain (PF23147; HMM-score: 14.5)TIM_barrel (CL0036) ICL; Isocitrate lyase family (PF00463; HMM-score: 13.5)OB (CL0021) DTP-pb9_B-dom; Distal tail protein pb9, B domain (PF21430; HMM-score: 13.1)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: unknown (no significant prediction)
- Cytoplasmic Score: 2.5
- Cytoplasmic Membrane Score: 2.5
- Cellwall Score: 2.5
- Extracellular Score: 2.5
- Internal Helices: 0
- DeepLocPro: Cytoplasmic Membrane
- Cytoplasmic Score: 0.0001
- Cytoplasmic Membrane Score: 0.9288
- Cell wall & surface Score: 0.0014
- Extracellular Score: 0.0697
- LocateP:
- SignalP: Signal peptide LIPO(Sec/SPII) length 19 aa
- SP(Sec/SPI): 0.000789
- TAT(Tat/SPI): 0.000052
- LIPO(Sec/SPII): 0.999024
- Cleavage Site: CS pos: 19-20. LTS-CQ. Pr: 0.9994
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
- GI:
- RefSeq:
- UniProt:
⊟Protein sequence[edit | edit source]
- MRIIKYLTILVISVVILTSCQSSSSQESTKSGEFRIVPTTVALTMTLDKLDLPIVGKPTSYKTLPNRYKDVPEIGQPMEPNVEAVKKLKPTHVLSVSTIKDEMQPFYKQLNMKGYFYDFDSLKGMQKSITQLGDQFNRKAQAKELNDHLNSVKQKIENKAAKQKKHPKVLILMGVPGSYLVATDKSYIGDLVKITGGENVIKVKDRQYISSNTENLLNINPDIILRLPHGMPEEVKKMFQKEFKQNDIWKHFKAVKNNHVYDLEEVPFGITANVDADKAMTQLYDLFYKDKK
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You can add further information about the gene and protein here. [edit]
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
Bioinformatics: 2018, 34(23);4118-4120
[PubMed:29931111] [WorldCat.org] [DOI] (I p) - ↑ Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
Curr Res Microb Sci: 2025, 9;100489
[PubMed:41146725] [WorldCat.org] [DOI] (I e)