PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_1032 [new locus tag: SAUSA300_RS05555 ]
• pan locus tag^?: SAUPAN003363000
• symbol: SAUSA300_1032
• pan gene symbol^?: isdE
• synonym:
• product: putative iron compound ABC transporter iron compound-binding protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_1032 [new locus tag: SAUSA300_RS05555 ]
• symbol: SAUSA300_1032
• product: putative iron compound ABC transporter iron compound-binding protein
• replicon: chromosome
• strand: +
• coordinates: 1127538..1128386
• length: 849
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3915144 NCBI
• RefSeq: YP_493730 NCBI
• BioCyc: see SAUSA300_RS05555
• MicrobesOnline: 1292547 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  GTGATAAGCGTCGTTATCTTAACCAGCTGTCAATCTTCCAGTTCTCAAGAATCAACTAAA
  TCCGGCGAATTCAGAATCGTACCAACAACTGTTGCATTGACAATGACATTGGACAAATTG
  GATTTACCAATTGTCGGCAAACCCACGTCATATAAGACATTGCCTAATCGTTATAAAGAT
  GTACCGGAAATTGGTCAACCAATGGAGCCGAATGTTGAAGCTGTTAAAAAGTTAAAACCA
  ACACATGTTTTGAGTGTGTCAACGATTAAAGATGAAATGCAACCATTTTACAAACAATTA
  AATATGAAAGGCTACTTTTATGATTTTGATAGTTTAAAAGGGATGCAAAAGTCGATTACA
  CAATTAGGTGATCAATTTAATCGTAAAGCACAAGCAAAAGAATTAAATGACCATTTAAAT
  TCTGTAAAGCAAAAAATTGAAAATAAAGCAGCTAAACAAAAGAAACATCCCAAAGTATTA
  ATATTAATGGGTGTACCGGGTAGCTATTTAGTAGCAACTGATAAATCATATATTGGTGAT
  TTAGTTAAAATAGCAGGTGGAGAAAATGTTATTAAAGTGAAAGATCGTCAATATATTTCG
  TCTAATACTGAAAATTTGTTGAATATCAATCCAGATATTATTTTACGATTACCACACGGA
  ATGCCTGAAGAAGTTAAGAAAATGTTTCAAAAAGAATTTAAACAAAATGATATTTGGAAA
  CATTTTAAAGCTGTGAAAAATAATCATGTTTATGACTTAGAGGAAGTGCCATTCGGTATT
  ACAGCAAATGTTGATGCTGATAAGGCAATGACTCAATTATATGATTTATTTTATAAGGAT
  AAAAAATAG
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⊟Protein

⊟General

• locus tag: SAUSA300_1032 [new locus tag: SAUSA300_RS05555 ]
• symbol: SAUSA300_1032
• description: putative iron compound ABC transporter iron compound-binding protein
• length: 282
• theoretical pI: 9.80489
• theoretical MW: 32056.9
• GRAVY: -0.50922

⊟Function

• TIGRFAM:
  heme ABC transporter, heme-binding protein isdE (TIGR03659; HMM-score: 405.6)
  and 2 more
  putative ABC transporter PGF-CTERM-modified substrate-binding protein (TIGR04281; HMM-score: 43.8)
  Genetic information processing Protein fate Protein and peptide secretion and trafficking twin arginine-targeting protein translocase TatB (TIGR01410; HMM-score: 15.1)
• TheSEED  :
  • Heme transporter IsdDEF, lipoprotein IsdE
  Iron acquisition and metabolism Iron acquisition and metabolism - no subcategory Heme, hemin uptake and utilization systems in GramPositives  Heme transporter IsdDEF, lipoprotein IsdE
• PFAM:
  Chelatase (CL0043) Peripla_BP_2; Periplasmic binding protein (PF01497; HMM-score: 138.8)
  and 4 more
  no clan defined Muted; Organelle biogenesis, Muted-like protein (PF14942; HMM-score: 17.2)
  HAD (CL0137) NT5C; 5' nucleotidase, deoxy (Pyrimidine), cytosolic type C protein (NT5C) (PF06941; HMM-score: 16.1)
  no clan defined DUF2098; Uncharacterized protein conserved in archaea (DUF2098) (PF09871; HMM-score: 15)
  PK_TIM (CL0151) ICL; Isocitrate lyase family (PF00463; HMM-score: 13.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors: heme b
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 0.83
  • Signal peptide possibility: -0.5
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.076423
  • TAT(Tat/SPI): 0.007373
  • LIPO(Sec/SPII): 0.157625
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87160253 NCBI
• RefSeq: YP_493730 NCBI
• UniProt: Q2FHU8 UniProt

⊟Protein sequence

• MISVVILTSCQSSSSQESTKSGEFRIVPTTVALTMTLDKLDLPIVGKPTSYKTLPNRYKDVPEIGQPMEPNVEAVKKLKPTHVLSVSTIKDEMQPFYKQLNMKGYFYDFDSLKGMQKSITQLGDQFNRKAQAKELNDHLNSVKQKIENKAAKQKKHPKVLILMGVPGSYLVATDKSYIGDLVKIAGGENVIKVKDRQYISSNTENLLNINPDIILRLPHGMPEEVKKMFQKEFKQNDIWKHFKAVKNNHVYDLEEVPFGITANVDADKAMTQLYDLFYKDKK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• SAUSA300_1030 > SAUSA300_1031 > SAUSA300_1032 > SAUSA300_1033 > srtB > SAUSA300_1035  

⊟Regulation

• regulator: Fur* (repression) regulon

  Fur*

  (TF)

  important in Iron homeostasis; RegPrecise    transcription unit transferred from N315 data RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications