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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1033 [new locus tag: SAUSA300_RS05560 ]
  • pan locus tag?: SAUPAN003364000
  • symbol: SAUSA300_1033
  • pan gene symbol?: isdF
  • synonym:
  • product: iron/heme permease

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_1033 [new locus tag: SAUSA300_RS05560 ]
  • symbol: SAUSA300_1033
  • product: iron/heme permease
  • replicon: chromosome
  • strand: +
  • coordinates: 1128399..1129364
  • length: 966
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGATAAAAAATAAAAAGAAACTACTATTTTTATGTTTGTTAGTCATTTTAATCGCAACT
    GCTTATATTTCGTTTGTAACCGGTACAATTAAATTGTCATTTAATGACCTATTTACAAAA
    TTTACAACTGGTAGCAATGAAGCAGTGGATTCAATCATTGATTTGCGATTGCCACGTATA
    TTAATTGCATTGATGGTTGGCGCAATGTTAGCAGTTTCTGGAGCATTATTACAAGCAGCA
    CTACAAAATCCTTTGGCAGAGGCGAATATCATTGGCGTTTCCTCAGGTGCACTTATAATG
    AGAGCACTTTGTATGTTGTTTATTCCACAATTGTACTTTTACTTACCATTATTAAGTTTT
    ATTGGAGGTTTAATACCATTTTTAATAATTATATTGTTGCATTCTAAATTTAGATTCAAT
    GCTGTAAGTATGATATTAGTAGGTGTTGCGTTATTCGTATTATTAAATGGTGTTTTAGAA
    ATTTTAACTCAAAACCCTTTAATGAAAATTCCTCAAGGCTTAACAATGAAAATATGGAGC
    GACGTATACATATTAGCAGTATCAGCATTATTGGGATTAATATTAACATTACTATTGTCC
    CCTAAATTGAATTTACTAAATTTAGACGACATACAAGCGCGAAGTATCGGTTTTAATATT
    GATCGTTACAGATGGTTAACAGGTTTATTAGCAGTATTTTTAGCAAGTGCAACTGTTGCG
    ATTGTTGGACAACTAGCCTTTTTAGGTATTATTGTGCCACATGTGGTTAGAAAGCTAGTT
    GGGGGCAATTACAGAGTACTTATTCCGTTTTCTACAGTTATTGGTGCATGGCTATTGTTA
    GTGGCTGATTTATTAGGACGAGTGATACAGCCTCCTTTAGAAATTCCAGCCAATGCTATT
    TTAATGATTGTCGGTGGTCCAATGCTAATTTACTTAATTTGTCAAAGTCAACGAAATCGA
    ATCTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    966

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_1033 [new locus tag: SAUSA300_RS05560 ]
  • symbol: SAUSA300_1033
  • description: iron/heme permease
  • length: 321
  • theoretical pI: 10.3065
  • theoretical MW: 35043.6
  • GRAVY: 1.1

⊟Function[edit | edit source]

  • TIGRFAM:
    proposed F420-0 ABC transporter, permease protein (TIGR03869; HMM-score: 124.9)
  • TheSEED  :
    • Heme transporter IsdDEF, permease component IsdF
    Iron acquisition and metabolism Iron acquisition and metabolism - no subcategory Heme, hemin uptake and utilization systems in GramPositives  Heme transporter IsdDEF, permease component IsdF
  • PFAM:
    Membrane_trans (CL0142) FecCD; FecCD transport family (PF01032; HMM-score: 248.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 9
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0
    • Signal peptide possibility: 0.5
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.152696
    • TAT(Tat/SPI): 0.000479
    • LIPO(Sec/SPII): 0.073727
  • predicted transmembrane helices (TMHMM): 8

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MIKNKKKLLFLCLLVILIATAYISFVTGTIKLSFNDLFTKFTTGSNEAVDSIIDLRLPRILIALMVGAMLAVSGALLQAALQNPLAEANIIGVSSGALIMRALCMLFIPQLYFYLPLLSFIGGLIPFLIIILLHSKFRFNAVSMILVGVALFVLLNGVLEILTQNPLMKIPQGLTMKIWSDVYILAVSALLGLILTLLLSPKLNLLNLDDIQARSIGFNIDRYRWLTGLLAVFLASATVAIVGQLAFLGIIVPHVVRKLVGGNYRVLIPFSTVIGAWLLLVADLLGRVIQPPLEIPANAILMIVGGPMLIYLICQSQRNRI

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: Fur* (repression) regulon
    Fur*(TF)important in Iron homeostasis; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Michelle L Reniere, Eric P Skaar
    Staphylococcus aureus haem oxygenases are differentially regulated by iron and haem.
    Mol Microbiol: 2008, 69(5);1304-15
    [PubMed:18643935] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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