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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01086
  • pan locus tag?: SAUPAN003364000
  • symbol: SAOUHSC_01086
  • pan gene symbol?: isdF
  • synonym:
  • product: iron compound ABC transporter permease

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_01086
  • symbol: SAOUHSC_01086
  • product: iron compound ABC transporter permease
  • replicon: chromosome
  • strand: +
  • coordinates: 1048276..1049052
  • length: 777
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGATAAAAAATAAAAAGAAACTACTATTTTTATGTTTGTTAGTCATTTTAATCGCAACT
    GCTTATATTTCGTTTGTAACCGGTACAATTAAATTGTCATTTAATGACCTATTTACAAAA
    TTTACAACTGGTAGCAATGAAGCAGTGGATTCAATCATTGATTTGCGATTGCCACGTATA
    TTAATTGCATTGATGGTTGGCGCAATGTTAGCAGTTTCTGGAGCATTATTACAAGCAGCA
    CTACAAAATCCTTTGGCAGAGGCGAATATCATTGGCGTTTCCTCAGGTGCACTTATAATG
    AGAGCACTTTGTATGTTGTTTATTCCACAATTGTACTTTTACTTACCATTATTAAGTTTT
    ATTGGAGGTTTAATACCATTTTTAATAATTATATTGTTGCATTCTAAATTTAGATTCAAT
    GCTGTAAGTATGATATTAGTAGGTGTTGCGTTATTCGTATTATTAAATGGTGTTTTAGAA
    ATTTTAACTCAAAACCCTTTAATGAAAATTCCTCAAGGCTTAACAATGAAAATATGGAGC
    GACGTATACATATTAGCAGTATCAGCATTATTGGGATTAATATTAACATTACTATTGTCC
    CCTAAATTGAATTTACTAAATTTAGACGACATACAAGCGCGAAGTATCGGTTTTAATATT
    GATCGTTACAGATGGTTAACAGGTTTATTAGCAGTATTTTTAGCAAGTGCAACTGTTGCG
    ATTGTTGGACAACTAGCCTTTTTAGGTATTATTGTGCACATGTGGTTAGAAAGCTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    777

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_01086
  • symbol: SAOUHSC_01086
  • description: iron compound ABC transporter permease
  • length: 258
  • theoretical pI: 9.91027
  • theoretical MW: 28244.3
  • GRAVY: 1.13798

⊟Function[edit | edit source]

  • TIGRFAM:
    proposed F420-0 ABC transporter, permease protein (TIGR03869; HMM-score: 67.5)
  • TheSEED  :
    • Heme transporter IsdDEF, permease component IsdF
    Iron acquisition and metabolism Iron acquisition and metabolism - no subcategory Heme, hemin uptake and utilization systems in GramPositives  Heme transporter IsdDEF, permease component IsdF
  • PFAM:
    Membrane_trans (CL0142) FecCD; FecCD transport family (PF01032; HMM-score: 172.2)
    and 1 more
    no clan defined DUF4131; Domain of unknown function (DUF4131) (PF13567; HMM-score: 8.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 7
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0
    • Signal peptide possibility: 0.5
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.152696
    • TAT(Tat/SPI): 0.000479
    • LIPO(Sec/SPII): 0.073727
  • predicted transmembrane helices (TMHMM): 7

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MIKNKKKLLFLCLLVILIATAYISFVTGTIKLSFNDLFTKFTTGSNEAVDSIIDLRLPRILIALMVGAMLAVSGALLQAALQNPLAEANIIGVSSGALIMRALCMLFIPQLYFYLPLLSFIGGLIPFLIIILLHSKFRFNAVSMILVGVALFVLLNGVLEILTQNPLMKIPQGLTMKIWSDVYILAVSALLGLILTLLLSPKLNLLNLDDIQARSIGFNIDRYRWLTGLLAVFLASATVAIVGQLAFLGIIVHMWLES

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: Fur* (repression) regulon
    Fur*(TF)important in Iron homeostasis; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.0 1.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)
  2. ↑ Michelle L Reniere, Eric P Skaar
    Staphylococcus aureus haem oxygenases are differentially regulated by iron and haem.
    Mol Microbiol: 2008, 69(5);1304-15
    [PubMed:18643935] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

John M Taylor, David E Heinrichs
Transferrin binding in Staphylococcus aureus: involvement of a cell wall-anchored protein.
Mol Microbiol: 2002, 43(6);1603-14
[PubMed:11952908] [WorldCat.org] [DOI] (P p)

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