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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1143 [new locus tag: SACOL_RS05835 ]
  • pan locus tag?: SAUPAN003363000
  • symbol: SACOL1143
  • pan gene symbol?: isdE
  • synonym:
  • product: iron compound ABC transporter iron compound-binding protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1143 [new locus tag: SACOL_RS05835 ]
  • symbol: SACOL1143
  • product: iron compound ABC transporter iron compound-binding protein
  • replicon: chromosome
  • strand: +
  • coordinates: 1151046..1151924
  • length: 879
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    TTGAGAATCATAAAGTATTTAACCATTTTAGTGATAAGCGTCGTTATCTTAACCAGCTGT
    CAATCTTCCAGTTCTCAAGAATCAACTAAATCCGGCGAATTCAGAATCGTACCAACAACT
    GTTGCATTGACAATGACATTGGACAAATTGGATTTACCAATTGTCGGCAAACCCACGTCA
    TATAAGACATTGCCTAATCGTTATAAAGATGTACCGGAAATTGGTCAACCAATGGAGCCG
    AATGTTGAAGCTGTTAAAAAGTTAAAACCAACACATGTTTTGAGTGTGTCAACGATTAAA
    GATGAAATGCAACCATTTTACAAACAATTAAATATGAAAGGCTACTTTTATGATTTTGAT
    AGTTTAAAAGGGATGCAAAAGTCGATTACACAATTAGGTGATCAATTTAATCGTAAAGCA
    CAAGCAAAAGAATTAAATGACCATTTAAATTCTGTAAAGCAAAAAATTGAAAATAAAGCA
    GCTAAACAAAAGAAACATCCCAAAGTATTAATATTAATGGGTGTACCGGGTAGCTATTTA
    GTAGCAACTGATAAATCATATATTGGTGATTTAGTTAAAATAGCAGGTGGAGAAAATGTT
    ATTAAAGTGAAAGATCGTCAATATATTTCGTCTAATACTGAAAATTTGTTGAATATCAAT
    CCAGATATTATTTTACGATTACCACACGGAATGCCTGAAGAAGTTAAGAAAATGTTTCAA
    AAAGAATTTAAACAAAATGATATTTGGAAACATTTTAAAGCTGTGAAAAATAATCATGTT
    TATGACTTAGAGGAAGTGCCATTCGGTATTACAGCAAATGTTGATGCTGATAAGGCAATG
    ACTCAATTATATGATTTATTTTATAAGGATAAAAAATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    879

⊟Protein[edit | edit source]

Protein Data Bank: 2Q8P
Protein Data Bank: 2Q8Q

⊟General[edit | edit source]

  • locus tag: SACOL1143 [new locus tag: SACOL_RS05835 ]
  • symbol: SACOL1143
  • description: iron compound ABC transporter iron compound-binding protein
  • length: 292
  • theoretical pI: 9.91166
  • theoretical MW: 33270.5
  • GRAVY: -0.440753

⊟Function[edit | edit source]

  • TIGRFAM:
    heme ABC transporter, heme-binding protein isdE (TIGR03659; HMM-score: 411.6)
    and 3 more
    putative ABC transporter PGF-CTERM-modified substrate-binding protein (TIGR04281; HMM-score: 43.7)
    Cell structure Cell envelope Other uncharacterized lipoprotein (TIGR02722; HMM-score: 13.1)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking twin arginine-targeting protein translocase TatB (TIGR01410; HMM-score: 7.5)
  • TheSEED  :
    • Heme transporter IsdDEF, lipoprotein IsdE
    Iron acquisition and metabolism Iron acquisition and metabolism - no subcategory Heme, hemin uptake and utilization systems in GramPositives  Heme transporter IsdDEF, lipoprotein IsdE
  • PFAM:
    Chelatase (CL0043) Peripla_BP_2; Periplasmic binding protein (PF01497; HMM-score: 144.4)
    and 5 more
    no clan defined Muted; Organelle biogenesis, Muted-like protein (PF14942; HMM-score: 18.2)
    HAD (CL0137) NT5C; 5' nucleotidase, deoxy (Pyrimidine), cytosolic type C protein (NT5C) (PF06941; HMM-score: 15.9)
    no clan defined DUF2098; Uncharacterized protein conserved in archaea (DUF2098) (PF09871; HMM-score: 14.9)
    GDH2_N; NAD-dependent glutamate dehydrogenase, N-terminal domain (PF23147; HMM-score: 14.5)
    TIM_barrel (CL0036) ICL; Isocitrate lyase family (PF00463; HMM-score: 13.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: heme b
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.0001
    • Cytoplasmic Membrane Score: 0.93
    • Cell wall & surface Score: 0.0014
    • Extracellular Score: 0.0685
  • LocateP: N-terminally anchored (No CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: -0.17
    • Signal peptide possibility: 1
    • N-terminally Anchored Score: 4
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: Signal peptide LIPO(Sec/SPII) length 19 aa
    • SP(Sec/SPI): 0.000789
    • TAT(Tat/SPI): 0.000052
    • LIPO(Sec/SPII): 0.999024
    • Cleavage Site: CS pos: 19-20. LTS-CQ. Pr: 0.9994
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MRIIKYLTILVISVVILTSCQSSSSQESTKSGEFRIVPTTVALTMTLDKLDLPIVGKPTSYKTLPNRYKDVPEIGQPMEPNVEAVKKLKPTHVLSVSTIKDEMQPFYKQLNMKGYFYDFDSLKGMQKSITQLGDQFNRKAQAKELNDHLNSVKQKIENKAAKQKKHPKVLILMGVPGSYLVATDKSYIGDLVKIAGGENVIKVKDRQYISSNTENLLNINPDIILRLPHGMPEEVKKMFQKEFKQNDIWKHFKAVKNNHVYDLEEVPFGITANVDADKAMTQLYDLFYKDKK

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Lipoprotein [1] [2]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: Fur* (repression) regulon
    Fur*(TF)important in Iron homeostasis; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  2. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  3. ↑ Michelle L Reniere, Eric P Skaar
    Staphylococcus aureus haem oxygenases are differentially regulated by iron and haem.
    Mol Microbiol: 2008, 69(5);1304-15
    [PubMed:18643935] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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