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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1141 [new locus tag: SACOL_RS05825 ]
  • pan locus tag?: SAUPAN003360000
  • symbol: isdC
  • pan gene symbol?: isdC
  • synonym:
  • product: NPQTN cell wall surface anchor protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1141 [new locus tag: SACOL_RS05825 ]
  • symbol: isdC
  • product: NPQTN cell wall surface anchor protein
  • replicon: chromosome
  • strand: +
  • coordinates: 1149290..1149973
  • length: 684
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    TTGAAAAATATTTTAAAAGTTTTTAATACAACGATTTTAGCGTTAATTATCATCATCGCG
    ACATTCAGTAATTCTGCAAATGCCGCAGATAGCGGTACTTTGAATTATGAGGTTTACAAA
    TACAATACCAATGACACGTCAATTGCTAATGACTATTTTAATAAACCGGCAAAGTACATT
    AAGAAAAATGGTAAATTGTATGTTCAAATAACTGTCAACCACAGTCATTGGATTACTGGA
    ATGAGTATCGAAGGACATAAAGAAAATATTATTAGTAAAAACACTGCCAAAGATGAACGC
    ACTTCTGAATTTGAAGTAAGTAAGTTGAACGGTAAAATAGATGGAAAAATTGACGTTTAT
    ATCGATGAAAAAGTAAATGGAAAGCCATTCAAATATGACCATCATTACAACATTACATAT
    AAATTTAATGGACCAACTGATGTAGCAGGTGCTAATGCACCAGGTAAAGATGATAAAAAT
    TCTGCTTCAGGTAGTGACAAAGGATCTGATGGAACGACTACTGGTCAAAGTGAATCTAAT
    AGTTCGAATAAAGACAAAGTAGAAAATCCACAAACAAATGCTGGTACACCTGCATATATA
    TATACAATACCAGTTGCATCCTTAGCATTATTAATCGCAATCACATTGTTTGTTAGAAAA
    AAATCTAAAGGCAATGTGGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    684

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL1141 [new locus tag: SACOL_RS05825 ]
  • symbol: IsdC
  • description: NPQTN cell wall surface anchor protein
  • length: 227
  • theoretical pI: 9.42324
  • theoretical MW: 24884.6
  • GRAVY: -0.57533

⊟Function[edit | edit source]

  • TIGRFAM:
    heme uptake protein IsdC (TIGR03656; HMM-score: 318.5)
    and 2 more
    sortase B signal domain, NPQTN class (TIGR03068; HMM-score: 87.7)
    heme uptake protein IsdB (TIGR03657; HMM-score: 15.2)
  • TheSEED  :
    • NPQTN cell wall anchored protein IsdC
    Iron acquisition and metabolism Iron acquisition and metabolism - no subcategory Heme, hemin uptake and utilization systems in GramPositives  NPQTN cell wall anchored protein IsdC
  • PFAM:
    no clan defined NEAT; Iron Transport-associated domain (PF05031; HMM-score: 88.8)
    and 1 more
    FBD (CL0289) SoxG; Sarcosine oxidase, gamma subunit family (PF04268; HMM-score: 12.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 3.33
    • Cellwall Score: 3.33
    • Extracellular Score: 3.33
    • Internal Helices: 2
  • LocateP: LPxTG Cell-wall anchored
    • Prediction by SwissProt Classification: Cell Wall
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0
    • Signal peptide possibility: 1
    • N-terminally Anchored Score: -2
    • Predicted Cleavage Site: found LPxTG motif :NPQTN
  • SignalP: Signal peptide SP(Sec/SPI) length 28 aa
    • SP(Sec/SPI): 0.998468
    • TAT(Tat/SPI): 0.000645
    • LIPO(Sec/SPII): 0.000521
    • Cleavage Site: CS pos: 28-29. ANA-AD. Pr: 0.9892
  • predicted transmembrane helices (TMHMM): 2

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKNILKVFNTTILALIIIIATFSNSANAADSGTLNYEVYKYNTNDTSIANDYFNKPAKYIKKNGKLYVQITVNHSHWITGMSIEGHKENIISKNTAKDERTSEFEVSKLNGKIDGKIDVYIDEKVNGKPFKYDHHYNITYKFNGPTDVAGANAPGKDDKNSASGSDKGSDGTTTGQSESNSSNKDKVENPQTNAGTPAYIYTIPVASLALLIAITLFVRKKSKGNVE

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Sortase substrate [1] [2]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: Fur* (repression) regulon
    Fur*(TF)important in Iron homeostasis; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  2. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  3. ↑ Michelle L Reniere, Eric P Skaar
    Staphylococcus aureus haem oxygenases are differentially regulated by iron and haem.
    Mol Microbiol: 2008, 69(5);1304-15
    [PubMed:18643935] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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