PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA0982 [new locus tag: SA_RS05565 ]
• pan locus tag^?: SAUPAN003367000
• symbol: srtB
• pan gene symbol^?: srtB
• synonym:
• product: NPQTN specific sortase B

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA0982 [new locus tag: SA_RS05565 ]
• symbol: srtB
• product: NPQTN specific sortase B
• replicon: chromosome
• strand: +
• coordinates: 1111733..1112467
• length: 735
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1123809 NCBI
• RefSeq: NP_374252 NCBI
• BioCyc: see SA_RS05565
• MicrobesOnline: 103278 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGAGAATGAAGCGATTTTTAACTATTGTACAAATTTTATTGGTTGTAATTATTATCATT
  TTTGGTTACAAAATTGTTCAAACATATATTGAAGACAAGCAAGAACGCGCAAATTATGAG
  AAATTACAACAAAAATTTCAAATGCTGATGAGCAAACATCAAGAACATGTGAGACCACAA
  TTTGAATCACTTGAAAAAATAAATAAAGACATTGTTGGATGGATAAAATTATCAGGAACA
  TCATTAAATTATCCAGTACTACAAGGTAAGACAAATCACGATTATTTAAATTTAGATTTT
  GAGCGAGAACATCGACGTAAAGGTAGTATTTTTATGGATTTTAGAAATGAATTGAAGAAT
  TTAAATCATAATACTATTTTATACGGGCACCATGTCGGTGATAATACGATGTTTGATGTG
  TTAGAAGATTATTTAAAGCAATCGTTTTATGAAAAACACAAGATAATTGAATTTGACAAT
  AAATATGGTAAATATCAATTGCAAGTATTTAGTGCATATAAAACTACTACTAAAGATAAT
  TACATACGTACAGATTTTGAAAATGATCAAGATTATCAACAATTTTTAGATGAGACAAAA
  CGTAAATCTGTAATTAATTCAGATGTTAATGTAACGGTAAAAGATAAAATAATGACTTTA
  TCAACGTGCGAAGATGCATATAGTGAAACAACGAAAAGAATTGTTGTTGTCGCAAAAATA
  ATTAAGGTAAGTTAA
  60
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  735
  

⊟Protein

• 1NG5
  PDB

Protein Data Bank: 1NG5

⊟General

• locus tag: SA0982 [new locus tag: SA_RS05565 ]
• symbol: SrtB
• description: NPQTN specific sortase B
• length: 244
• theoretical pI: 9.23187
• theoretical MW: 29135.2
• GRAVY: -0.580328

⊟Function

• TIGRFAM:
  Cell structure Cell envelope Other sortase, SrtB family (TIGR03064; EC 3.4.22.71; HMM-score: 304.1)
  Genetic information processing Protein fate Protein and peptide secretion and trafficking sortase, SrtB family (TIGR03064; EC 3.4.22.71; HMM-score: 304.1)
  and 2 more
  Cell structure Cell envelope Other sortase (TIGR01076; EC 3.4.22.-; HMM-score: 13.1)
  Genetic information processing Protein fate Protein and peptide secretion and trafficking sortase (TIGR01076; EC 3.4.22.-; HMM-score: 13.1)
• TheSEED  :
  • NPQTN specific sortase B
  Cell Wall and Capsule Gram-Positive cell wall components Sortase  NPQTN specific sortase B
  and 1 more
  Iron acquisition and metabolism Iron acquisition and metabolism - no subcategory Heme, hemin uptake and utilization systems in GramPositives  NPQTN specific sortase B
• PFAM:
  no clan defined Sortase; Sortase family (PF04203; HMM-score: 86.8)
  and 4 more
  TPR (CL0020) TPR_21; Tetratricopeptide repeat-like domain (PF09976; HMM-score: 13.7)
  C_Lectin (CL0056) Ly49; Ly49-like protein, N-terminal region (PF08391; HMM-score: 10.6)
  no clan defined HJURP_C; Holliday junction regulator protein family C-terminal repeat (PF12347; HMM-score: 10.3)
  Arc_PepC_II; Archaeal Peptidase A24 C-terminus Type II (PF06847; HMM-score: 9.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helix: 1
• LocateP: N-terminally anchored (No CS)
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: 0
  • N-terminally Anchored Score: 4
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.309695
  • TAT(Tat/SPI): 0.000617
  • LIPO(Sec/SPII): 0.021892
• predicted transmembrane helices (TMHMM): 1

⊟Accession numbers

• GI: 15926719 NCBI
• RefSeq: NP_374252 NCBI
• UniProt: A0A0H3JPP1 UniProt

⊟Protein sequence

• MRMKRFLTIVQILLVVIIIIFGYKIVQTYIEDKQERANYEKLQQKFQMLMSKHQEHVRPQFESLEKINKDIVGWIKLSGTSLNYPVLQGKTNHDYLNLDFEREHRRKGSIFMDFRNELKNLNHNTILYGHHVGDNTMFDVLEDYLKQSFYEKHKIIEFDNKYGKYQLQVFSAYKTTTKDNYIRTDFENDQDYQQFLDETKRKSVINSDVNVTVKDKIMTLSTCEDAYSETTKRIVVVAKIIKVS

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• isdC > isdD > isdE > isdF > srtB > isdG  

⊟Regulation

• regulator: Fur* (repression) regulon

  Fur*

  (TF)

  important in Iron homeostasis; RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Rongguang Zhang, Ruiying Wu, Grazyna Joachimiak, Sarkis K Mazmanian, Dominique M Missiakas, Piotr Gornicki, Olaf Schneewind, Andrzej Joachimiak
Structures of sortase B from Staphylococcus aureus and Bacillus anthracis reveal catalytic amino acid triad in the active site.
Structure: 2004, 12(7);1147-56
[PubMed:15242591] [WorldCat.org] [DOI] (P p)Matthew L Bentley, Helena Gaweska, Joseph M Kielec, Dewey G McCafferty
Engineering the substrate specificity of Staphylococcus aureus Sortase A. The beta6/beta7 loop from SrtB confers NPQTN recognition to SrtA.
J Biol Chem: 2007, 282(9);6571-81
[PubMed:17200112] [WorldCat.org] [DOI] (P p)