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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_001006
  • pan locus tag?: SAUPAN003244000
  • symbol: JSNZ_001006
  • pan gene symbol?: menF
  • synonym:
  • product: isochorismate synthase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_001006
  • symbol: JSNZ_001006
  • product: isochorismate synthase
  • replicon: chromosome
  • strand: +
  • coordinates: 1006923..1008245
  • length: 1323
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATATATGGCAGCTCAAAGGAATGGGTTTCAGTTGAAGTGAAATTATCACAGTCACTAGAC
    CCGAGCACATTATTTCATCTCACTGACAATGAGGCAGGAGATCGCTTTTATATGCGTTTG
    AATGATAATCGAACGTCATATTTTGGCTACAAAGCAATTCAATTATTCAAAAATAATTCT
    AAAAATAAACAATCTATTTTTAAAGACTGGGAAAAATTAAAACATAACATCACATTTATA
    CATCCGCAATCTGAGAAACATCATCTTCGAGTTGTTGGAGGGTTTCAATTTTCAAGTCAT
    AAATCAGATGATGAATGGCGAGAGTTTGGACTAAATCATTTTGTATTACCTGAAGTTTTA
    ATTTCAACTGATAATAATGGGACATTTTTAACTTATACAGTTAAAAGGGAAAGTTTTACT
    GTTGAGGCATTGAACGATTTAATGGATTTGTTCAACAATATATCGGATATAGATGTGGAC
    GAGCAAATTGGGGAAATTACTAGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTT
    GTAGTAGAAGCTATAGAATCTATTAATAATGAAGAAAAAATTGTACTAGCACGTAGACGG
    TTAATAAAGTTCGATAAAGATATCAGTATTCCATATATTCTAAAGCAAGCATATTCTAAA
    GAAAAAAACAGTTATATATTCTTGTTAGAATCACAAGATTCTATTTTCTTTTCACAAACA
    CCTGAACAATTAATAAAGGTCAATAATAAAATACTATCGACTAAAGCTGTAGCAGGTACA
    ATTAAACGTTCACAAGATGAGGACGAAGATACAAAAAATGTTGAAGCATTTTTAAAAGAT
    AATAAAAACTTAATCGAACATCGATTTGTTGTTGACAGTATTTTACATGATATTAAACCT
    TATATCACTGAATTACATTATGATAAGACGCCTAAAATTCTAAAAAATGATCATTTATAT
    CACTTGTACACTGAAATAAAGGCGCCACTGAAGGATGATTCGTATATTAGTTTAATTGAT
    CATTTACATCCAACACCTGCTTTAGGTGGCTATCCAAAAGAATTTGCGATGGATTTTATT
    GAACAGAAAGAATTTGGTACACGAGGATTATATGGTGCGCCGGTTGGCTATATAGATATA
    TATGATGATTGTGAATTTATTGTTGCAATTCGTTCGATGCTTATTAAGAAAGCACAAGCA
    ACTTTATTTGCTGGGTGTGGCATTGTTAAAGATTCTGATCCAGATAGTGAATTGGCAGAA
    ACGAACCTTAAGTTCACACCTATGATGAATGCATTAGGAGTCGATATGAATGGGAAATCA
    TAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1323

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_001006
  • symbol: JSNZ_001006
  • description: isochorismate synthase
  • length: 440
  • theoretical pI: 5.36104
  • theoretical MW: 50861.2
  • GRAVY: -0.482045

⊟Function[edit | edit source]

  • reaction:
    EC 5.4.4.2?  ExPASy
    Isochorismate synthase Chorismate = isochorismate
  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone isochorismate synthase (TIGR00543; EC 5.4.4.2; HMM-score: 412.1)
    and 8 more
    Metabolism Amino acid biosynthesis Aromatic amino acid family anthranilate synthase component I (TIGR01820; EC 4.1.3.27; HMM-score: 152.7)
    Metabolism Amino acid biosynthesis Aromatic amino acid family anthranilate synthase component I (TIGR00564; EC 4.1.3.27; HMM-score: 139.2)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Folic acid aminodeoxychorismate synthase, component I (TIGR00553; EC 2.6.1.85; HMM-score: 118.1)
    aminodeoxychorismate synthase, component I (TIGR01824; EC 2.6.1.85; HMM-score: 106.6)
    salicylate synthase (TIGR03494; EC 4.1.3.-,5.4.4.2; HMM-score: 90)
    Metabolism Amino acid biosynthesis Aromatic amino acid family anthranilate synthase component I (TIGR00565; EC 4.1.3.27; HMM-score: 82.9)
    Metabolism Amino acid biosynthesis Aromatic amino acid family anthranilate synthase (TIGR01815; EC 4.1.3.27; HMM-score: 61.7)
    aminodeoxychorismate synthase (TIGR01823; EC 2.6.1.85; HMM-score: 60.5)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    no clan defined Chorismate_bind; chorismate binding enzyme (PF00425; HMM-score: 232.8)
    and 2 more
    ATP_synthase (CL0255) vATP-synt_E; ATP synthase (E/31 kDa) subunit (PF01991; HMM-score: 16.5)
    HTH (CL0123) SinI; Anti-repressor SinI (PF08671; HMM-score: 9.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.7046
    • Cytoplasmic Membrane Score: 0.2385
    • Cell wall & surface Score: 0.0018
    • Extracellular Score: 0.0552
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.005027
    • TAT(Tat/SPI): 0.000253
    • LIPO(Sec/SPII): 0.000904
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MYGSSKEWVSVEVKLSQSLDPSTLFHLTDNEAGDRFYMRLNDNRTSYFGYKAIQLFKNNSKNKQSIFKDWEKLKHNITFIHPQSEKHHLRVVGGFQFSSHKSDDEWREFGLNHFVLPEVLISTDNNGTFLTYTVKRESFTVEALNDLMDLFNNISDIDVDEQIGEITRNEDIYKDDWRQLVVEAIESINNEEKIVLARRRLIKFDKDISIPYILKQAYSKEKNSYIFLLESQDSIFFSQTPEQLIKVNNKILSTKAVAGTIKRSQDEDEDTKNVEAFLKDNKNLIEHRFVVDSILHDIKPYITELHYDKTPKILKNDHLYHLYTEIKAPLKDDSYISLIDHLHPTPALGGYPKEFAMDFIEQKEFGTRGLYGAPVGYIDIYDDCEFIVAIRSMLIKKAQATLFAGCGIVKDSDPDSELAETNLKFTPMMNALGVDMNGKS

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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