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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_001008
  • pan locus tag?: SAUPAN003247000
  • symbol: menH
  • pan gene symbol?: menH
  • synonym:
  • product: 2-succinyl-6-hydroxy-2, 4-cyclohexadiene-1-carboxylate synthase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_001008
  • symbol: menH
  • product: 2-succinyl-6-hydroxy-2, 4-cyclohexadiene-1-carboxylate synthase
  • replicon: chromosome
  • strand: +
  • coordinates: 1009892..1010695
  • length: 804
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGACACATTATAATTACTATGAAGCAAACGTTGAGACCAATCAAGTTTTAGTATTACTG
    CATGGTTTTCTTAGCGACAGTCGTACTTATCATAATCACATCGATAAATATACTGATATC
    TGTCATGTCATCACTATAGACATACCAGGCCATGGCGAAGATCAGTCTTCAATGGATGAA
    ACGTGGAATTTTGATTATATTACGACGTTGTTAGACCGAATTTTAGATAAATATAAAGAT
    AAATCAATAACATTGTTTGGATATTCAATGGGTGGGCGTGTTGCATTATATTATGCAATT
    AATGGTCACATCCCTATATCTAATTTGATATTAGAAAGTACGTCACCAGGTATTAAAGAA
    GAAGCAAATCAATTGGAACGCCGTCTTGTTGATGAAGCGCGTGCTAAAGTATTAGACATA
    GCAGGTATTGAATTATTTGTTAATGATTGGGAAAAGTTGCCATTATTTCAATCGCAAAAA
    GGTTTACCAGTTGAAATACAACATCAAATAAGACAACAACGATTGTCTCAATCGCCACAG
    AAAATGGCCAAAGCATTAAGAGATTATGGTACAGGTCAAATGCCAAACTTATGGCCGCGC
    CTGAAAGAAATTAAAGTACCAACATTAATATTAGCTGGAGAATATGATGGAAAATTTGTA
    CAGATTGCGAAAAAAATGGCAAATTTAATTCCTAATAGTAAATGTAAATTAATTTCTGCT
    ACAGGTCATACAATTCATGTGGAAGATAGTGATGAATTTGATACAATGATATTAGGATTT
    TTAAAGGAGGAGCAAAATGACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    804

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_001008
  • symbol: MenH
  • description: 2-succinyl-6-hydroxy-2, 4-cyclohexadiene-1-carboxylate synthase
  • length: 267
  • theoretical pI: 5.78341
  • theoretical MW: 30569.6
  • GRAVY: -0.395131

⊟Function[edit | edit source]

  • reaction:
    EC 4.2.99.20?  ExPASy
    2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase 5-enolpyruvoyl-6-hydroxy-2-succinylcyclohex-3-ene-1-carboxylate = (1R,6R)-6-hydroxy-2-succinylcyclohexa-2,4-diene-1-carboxylate + pyruvate
  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase (TIGR03695; EC 4.2.99.20; HMM-score: 292.8)
    and 9 more
    Metabolism Energy metabolism Other 3-oxoadipate enol-lactonase (TIGR02427; EC 3.1.1.24; HMM-score: 76.2)
    proline-specific peptidase (TIGR01250; HMM-score: 74.4)
    Metabolism Energy metabolism Photosynthesis putative magnesium chelatase accessory protein (TIGR03056; HMM-score: 56.8)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Biotin pimelyl-[acyl-carrier protein] methyl ester esterase (TIGR01738; EC 3.1.1.85; HMM-score: 51.8)
    pyrimidine utilization protein D (TIGR03611; HMM-score: 51.3)
    Metabolism Energy metabolism Other poly(3-hydroxyalkanoate) depolymerase (TIGR02240; EC 3.1.1.-; HMM-score: 36.7)
    2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase (TIGR03343; EC 3.7.1.-; HMM-score: 36)
    Metabolism Fatty acid and phospholipid metabolism Biosynthesis poly(R)-hydroxyalkanoic acid synthase, class III, PhaC subunit (TIGR01836; HMM-score: 21.2)
    Metabolism Amino acid biosynthesis Aspartate family homoserine O-acetyltransferase (TIGR01392; EC 2.3.1.31; HMM-score: 19.8)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    AB_hydrolase (CL0028) Abhydrolase_1; alpha/beta hydrolase fold (PF00561; HMM-score: 94.8)
    and 13 more
    Abhydrolase_6; Alpha/beta hydrolase family (PF12697; HMM-score: 59.7)
    Hydrolase_4; Serine aminopeptidase, S33 (PF12146; HMM-score: 53.2)
    Thioesterase; Thioesterase domain (PF00975; HMM-score: 23.5)
    PepSY (CL0320) Staphopain_pro; Staphopain proregion (PF14731; HMM-score: 20.6)
    AB_hydrolase (CL0028) Ndr; Ndr family (PF03096; HMM-score: 19.7)
    Abhydrolase_11; Alpha/beta hydrolase domain (PF20408; HMM-score: 19.4)
    PGAP1; PGAP1-like alpha/beta domain (PF07819; HMM-score: 19)
    Esterase; Putative esterase (PF00756; HMM-score: 18.3)
    Lipase_3; Lipase (class 3) (PF01764; HMM-score: 16.6)
    Mbeg1-like; Mbeg1-like (PF11187; HMM-score: 16.6)
    Abhydrolase_2; Phospholipase/Carboxylesterase (PF02230; HMM-score: 16.2)
    DUF915; Alpha/beta hydrolase of unknown function (DUF915) (PF06028; HMM-score: 14.7)
    DLH; Dienelactone hydrolase family (PF01738; HMM-score: 12.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9267
    • Cytoplasmic Membrane Score: 0.0417
    • Cell wall & surface Score: 0.0022
    • Extracellular Score: 0.0294
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.00842
    • TAT(Tat/SPI): 0.000249
    • LIPO(Sec/SPII): 0.001801
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MTHYNYYEANVETNQVLVLLHGFLSDSRTYHNHIDKYTDICHVITIDIPGHGEDQSSMDETWNFDYITTLLDRILDKYKDKSITLFGYSMGGRVALYYAINGHIPISNLILESTSPGIKEEANQLERRLVDEARAKVLDIAGIELFVNDWEKLPLFQSQKGLPVEIQHQIRQQRLSQSPQKMAKALRDYGTGQMPNLWPRLKEIKVPTLILAGEYDGKFVQIAKKMANLIPNSKCKLISATGHTIHVEDSDEFDTMILGFLKEEQND

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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