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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_0945 [new locus tag: SAUSA300_RS05080 ]
  • pan locus tag?: SAUPAN003244000
  • symbol: SAUSA300_0945
  • pan gene symbol?: menF
  • synonym:
  • product: isochorismate synthase family protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_0945 [new locus tag: SAUSA300_RS05080 ]
  • symbol: SAUSA300_0945
  • product: isochorismate synthase family protein
  • replicon: chromosome
  • strand: +
  • coordinates: 1033198..1034409
  • length: 1212
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGCGTTTGAATGATAATCGAACGTCATATTTTGGCTACAAAGCAATTCAATTATTCAAA
    AATAATTCTAAAAATAAACAATCTATTTTTAAAGACTGGGAAAAATTAAAACATAACATC
    ACATTTATACATCCGCAATCTGAGAAACATCATCTTCGAGTTGTTGGAGGGTTCCAATTT
    TCAAGTCATAAATCAGATGATGAATGGCGAGAGTTTGGACTAAATCATTTTGTATTACCT
    GAAGTTTTAATTTCAACTGATAATAATGGGACATTTTTAACTTATACAGTTAAAAGGGAA
    AGTTTTACTGTTGAGGCATTGAACGATTTAATGGATTTGTTCAACAATATATCGGACATA
    GATGTGGACGAGCAAATTGGGGGAATTACTAGAAATGAAGATATTTATAAAGATGACTGG
    CGTCAACTTGTAGTAGAAGCTATAGAATCTATTAATAATGAAGAAAAAATTGTACTAGCA
    CGTAGACGGTTAATAAAGTTCGATAAAGATATCAGTATTCCATATATTCTAAAGCAAGCA
    TATTCTAAAGAAAAAAACAGTTATATATTCTTGTTAGAATCACAAGATTCTATATTCTTT
    TCACAAACACCTGAACAATTAATAAAGGTCAATAATAAAATACTATCGACTAAAGCTGTA
    GCAGGTACAATTAAACGTTCACAAGATGAGGACGAAGATACAAAAAATGTTGAAGCATTT
    TTAAAAGATAATAAAAACTTAATCGAACATCGATTTGTTGTTGACAGTATTTTACATGAT
    ATTAAACCTTATATCACTGAATTACATTATGATAAGACGCCTAAAATTCTAAAAAATGAT
    CATTTATATCACTTGTACACTGAAATAAAGGCGCCACTGAAGGATGATTCGTATATTAGT
    TTAATTGATAATTTACATCCAACACCTGCTTTAGGTGGCTATCCAAAAGAATTTGCAATG
    GATTTTATTGAGCAGAAAGAATTTGGTACGCGAGGATTATATGGTGCACCGGTTGGCTAT
    ATAGATATATACGATGATTGTGAATTTATTGTTGCAATTCGTTCGATGCTTATTAAGAAA
    GCACAAGCAACTTTATTTGCTGGGTGTGGCATTGTTAAAGATTCTGATCCAGATAGTGAA
    TTGGCAGAAACGAACCTTAAGTTCACACCTATGATGAATGCATTAGGAGTCGATATGAAT
    GGGAAATCATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1212

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_0945 [new locus tag: SAUSA300_RS05080 ]
  • symbol: SAUSA300_0945
  • description: isochorismate synthase family protein
  • length: 403
  • theoretical pI: 5.63334
  • theoretical MW: 46546.5
  • GRAVY: -0.473449

⊟Function[edit | edit source]

  • reaction:
    EC 5.4.4.2?  ExPASy
    Isochorismate synthase Chorismate = isochorismate
  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone isochorismate synthase (TIGR00543; EC 5.4.4.2; HMM-score: 409.7)
    and 8 more
    Metabolism Amino acid biosynthesis Aromatic amino acid family anthranilate synthase component I (TIGR01820; EC 4.1.3.27; HMM-score: 152.6)
    Metabolism Amino acid biosynthesis Aromatic amino acid family anthranilate synthase component I (TIGR00564; EC 4.1.3.27; HMM-score: 138.6)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Folic acid aminodeoxychorismate synthase, component I (TIGR00553; EC 2.6.1.85; HMM-score: 118.9)
    aminodeoxychorismate synthase, component I (TIGR01824; EC 2.6.1.85; HMM-score: 107.3)
    salicylate synthase (TIGR03494; EC 4.1.3.-,5.4.4.2; HMM-score: 89.1)
    Metabolism Amino acid biosynthesis Aromatic amino acid family anthranilate synthase component I (TIGR00565; EC 4.1.3.27; HMM-score: 83.1)
    Metabolism Amino acid biosynthesis Aromatic amino acid family anthranilate synthase (TIGR01815; EC 4.1.3.27; HMM-score: 60.1)
    aminodeoxychorismate synthase (TIGR01823; EC 2.6.1.85; HMM-score: 59.3)
  • TheSEED  :
    • Isochorismate synthase (EC 5.4.4.2)
    • Menaquinone-specific isochorismate synthase (EC 5.4.4.2)
    Cofactors, Vitamins, Prosthetic Groups, Pigments Quinone cofactors Menaquinone and Phylloquinone Biosynthesis  Menaquinone-specific isochorismate synthase (EC 5.4.4.2)
    and 1 more
    Cofactors, Vitamins, Prosthetic Groups, Pigments Quinone cofactors Menaquinone biosynthesis from chorismate via 1,4-dihydroxy-2-naphthoate  Menaquinone-specific isochorismate synthase (EC 5.4.4.2)
  • PFAM:
    no clan defined Chorismate_bind; chorismate binding enzyme (PF00425; HMM-score: 217.5)
    and 2 more
    ATP_synthase (CL0255) vATP-synt_E; ATP synthase (E/31 kDa) subunit (PF01991; HMM-score: 15.8)
    no clan defined SinI; Anti-repressor SinI (PF08671; HMM-score: 9.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004377
    • TAT(Tat/SPI): 0.000212
    • LIPO(Sec/SPII): 0.000715
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MRLNDNRTSYFGYKAIQLFKNNSKNKQSIFKDWEKLKHNITFIHPQSEKHHLRVVGGFQFSSHKSDDEWREFGLNHFVLPEVLISTDNNGTFLTYTVKRESFTVEALNDLMDLFNNISDIDVDEQIGGITRNEDIYKDDWRQLVVEAIESINNEEKIVLARRRLIKFDKDISIPYILKQAYSKEKNSYIFLLESQDSIFFSQTPEQLIKVNNKILSTKAVAGTIKRSQDEDEDTKNVEAFLKDNKNLIEHRFVVDSILHDIKPYITELHYDKTPKILKNDHLYHLYTEIKAPLKDDSYISLIDNLHPTPALGGYPKEFAMDFIEQKEFGTRGLYGAPVGYIDIYDDCEFIVAIRSMLIKKAQATLFAGCGIVKDSDPDSELAETNLKFTPMMNALGVDMNGKS

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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