PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_RS05080 [old locus tag: SAUSA300_0945 ]
• pan locus tag^?: SAUPAN003244000
• symbol: SAUSA300_RS05080
• pan gene symbol^?: menF
• synonym:
• product: isochorismate synthase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_RS05080 [old locus tag: SAUSA300_0945 ]
• symbol: SAUSA300_RS05080
• product: isochorismate synthase
• replicon: chromosome
• strand: +
• coordinates: 1033198..1034409
• length: 1212
• essential: unknown other strains

⊟Accession numbers

• Location: NC_007793 (1033198..1034409) NCBI
• BioCyc: SAUSA300_RS05080 BioCyc
• MicrobesOnline: see SAUSA300_0945

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGCGTTTGAATGATAATCGAACGTCATATTTTGGCTACAAAGCAATTCAATTATTCAAA
  AATAATTCTAAAAATAAACAATCTATTTTTAAAGACTGGGAAAAATTAAAACATAACATC
  ACATTTATACATCCGCAATCTGAGAAACATCATCTTCGAGTTGTTGGAGGGTTCCAATTT
  TCAAGTCATAAATCAGATGATGAATGGCGAGAGTTTGGACTAAATCATTTTGTATTACCT
  GAAGTTTTAATTTCAACTGATAATAATGGGACATTTTTAACTTATACAGTTAAAAGGGAA
  AGTTTTACTGTTGAGGCATTGAACGATTTAATGGATTTGTTCAACAATATATCGGACATA
  GATGTGGACGAGCAAATTGGGGGAATTACTAGAAATGAAGATATTTATAAAGATGACTGG
  CGTCAACTTGTAGTAGAAGCTATAGAATCTATTAATAATGAAGAAAAAATTGTACTAGCA
  CGTAGACGGTTAATAAAGTTCGATAAAGATATCAGTATTCCATATATTCTAAAGCAAGCA
  TATTCTAAAGAAAAAAACAGTTATATATTCTTGTTAGAATCACAAGATTCTATATTCTTT
  TCACAAACACCTGAACAATTAATAAAGGTCAATAATAAAATACTATCGACTAAAGCTGTA
  GCAGGTACAATTAAACGTTCACAAGATGAGGACGAAGATACAAAAAATGTTGAAGCATTT
  TTAAAAGATAATAAAAACTTAATCGAACATCGATTTGTTGTTGACAGTATTTTACATGAT
  ATTAAACCTTATATCACTGAATTACATTATGATAAGACGCCTAAAATTCTAAAAAATGAT
  CATTTATATCACTTGTACACTGAAATAAAGGCGCCACTGAAGGATGATTCGTATATTAGT
  TTAATTGATAATTTACATCCAACACCTGCTTTAGGTGGCTATCCAAAAGAATTTGCAATG
  GATTTTATTGAGCAGAAAGAATTTGGTACGCGAGGATTATATGGTGCACCGGTTGGCTAT
  ATAGATATATACGATGATTGTGAATTTATTGTTGCAATTCGTTCGATGCTTATTAAGAAA
  GCACAAGCAACTTTATTTGCTGGGTGTGGCATTGTTAAAGATTCTGATCCAGATAGTGAA
  TTGGCAGAAACGAACCTTAAGTTCACACCTATGATGAATGCATTAGGAGTCGATATGAAT
  GGGAAATCATAA
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⊟Protein

⊟General

• locus tag: SAUSA300_RS05080 [old locus tag: SAUSA300_0945 ]
• symbol: SAUSA300_RS05080
• description: isochorismate synthase
• length: 403
• theoretical pI: 5.63334
• theoretical MW: 46546.5
• GRAVY: -0.473449

⊟Function

• reaction:
  EC 5.4.4.2?  ExPASy
  Isochorismate synthase Chorismate = isochorismate
• TIGRFAM:
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone isochorismate synthase (TIGR00543; EC 5.4.4.2; HMM-score: 409.7)
  and 8 more
  Metabolism Amino acid biosynthesis Aromatic amino acid family anthranilate synthase component I (TIGR01820; EC 4.1.3.27; HMM-score: 152.6)
  Metabolism Amino acid biosynthesis Aromatic amino acid family anthranilate synthase component I (TIGR00564; EC 4.1.3.27; HMM-score: 138.6)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Folic acid aminodeoxychorismate synthase, component I (TIGR00553; EC 2.6.1.85; HMM-score: 118.9)
  aminodeoxychorismate synthase, component I (TIGR01824; EC 2.6.1.85; HMM-score: 107.3)
  salicylate synthase (TIGR03494; EC 4.1.3.-,5.4.4.2; HMM-score: 89.1)
  Metabolism Amino acid biosynthesis Aromatic amino acid family anthranilate synthase component I (TIGR00565; EC 4.1.3.27; HMM-score: 83.1)
  Metabolism Amino acid biosynthesis Aromatic amino acid family anthranilate synthase (TIGR01815; EC 4.1.3.27; HMM-score: 60.1)
  aminodeoxychorismate synthase (TIGR01823; EC 2.6.1.85; HMM-score: 59.3)
• TheSEED: see SAUSA300_0945
• PFAM:
  no clan defined Chorismate_bind; chorismate binding enzyme (PF00425; HMM-score: 217.5)
  and 2 more
  ATP_synthase (CL0255) vATP-synt_E; ATP synthase (E/31 kDa) subunit (PF01991; HMM-score: 15.8)
  no clan defined SinI; Anti-repressor SinI (PF08671; HMM-score: 9.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.004377
  • TAT(Tat/SPI): 0.000212
  • LIPO(Sec/SPII): 0.000715
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: see SAUSA300_0945
• RefSeq: WP_011443628 NCBI
• UniProt: see SAUSA300_0945

⊟Protein sequence

• MRLNDNRTSYFGYKAIQLFKNNSKNKQSIFKDWEKLKHNITFIHPQSEKHHLRVVGGFQFSSHKSDDEWREFGLNHFVLPEVLISTDNNGTFLTYTVKRESFTVEALNDLMDLFNNISDIDVDEQIGGITRNEDIYKDDWRQLVVEAIESINNEEKIVLARRRLIKFDKDISIPYILKQAYSKEKNSYIFLLESQDSIFFSQTPEQLIKVNNKILSTKAVAGTIKRSQDEDEDTKNVEAFLKDNKNLIEHRFVVDSILHDIKPYITELHYDKTPKILKNDHLYHLYTEIKAPLKDDSYISLIDNLHPTPALGGYPKEFAMDFIEQKEFGTRGLYGAPVGYIDIYDDCEFIVAIRSMLIKKAQATLFAGCGIVKDSDPDSELAETNLKFTPMMNALGVDMNGKS

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications