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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_000802
  • pan locus tag?: SAUPAN002895000
  • symbol: sufD
  • pan gene symbol?: sufD
  • synonym:
  • product: Fe-S cluster assembly protein SufD

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_000802
  • symbol: sufD
  • product: Fe-S cluster assembly protein SufD
  • replicon: chromosome
  • strand: +
  • coordinates: 831945..833252
  • length: 1308
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGACAACTGATATTTTGAACATTTCTGAAGAACAACTTGTTGATTATTCTAAAGCCCAC
    AATGAACCTTCTTGGATGACAGAATTACGTAAAAAAGCTTTGAAATTAACAGAAACTTTA
    GAAATGCCAAAACCTGATAAAACAAAATTAAGAAAATGGGATTTTGAATCTTTTAAACAA
    CACGATGTAAAAGGTGATGTTTATCAATCTTTATCACAATTACCTGAGTCAGTAAGAGAA
    ATTATTGACGTAGATCATTCTAAAAACTTAGTAATTCAACATAATAATACGATTGCGTAC
    ACACAAGTTGATGATAATGCATCGAAAGATGGCGTTATCGTTGAAGGTTTAGCAGACGCT
    CTTATGAACCATAGTGATTTAGTACAAAAGTACTTTATGAAAGATGCAGTAACAGTAGAT
    GAACATCGTATCACAGCGCTACACACGGCATTAGTTAATGGTGGCGTATTTGTTTATGTT
    CCTAAAAATGTAGTTGTAGAACATCCAGTACAATACGTTGTGTTGCACGACGACGAAAAT
    GCAAGCTTTTATAACCATGTTATCATCGTTACTGAAGAAAGCGCCGAAGTCACATATGTT
    GAAAATTACTTATCAAATGCATCTGGTGAAGGAAATCAATTAAATATTATTTCTGAAGTG
    ATTGCTGGCGCAAACTCCAATATCACATATGGCTCAGTGGACTATATGGATAAAGGCTTT
    ACAGGTCATATCATTCGACGTGGTATTACTGAAGCGGATGCCTCAATTAATTGGGCATTA
    GGTTTAATGAATGAGGGTAGCCAAATTATTGATAATACAACAAATTTATTTGGTGATCGT
    TCAACAAGTTCACTTAAATCAGTAGTTGTTGGTACAGGCGAACAAAAAATTAATCTAACA
    TCTAAAATCGTACAATATGGTAAAGAAACAGATGGTTATATCCTTAAACATGGTGTTATG
    AAAGAACATGCATCATCTGTATTTAATGGTATCGGCTACATTAAGCATGGTGGAACTAAA
    TCAATTGCTAATCAGGAATCACGTGTATTAATGTTATCTGAACATGCTCGTGGTGACGCG
    AATCCTATTTTATTAATTGATGAAGATGATGTACAAGCTGGTCATGCTGCATCAGTAGGT
    CGTGTTGATCCAGATCAACTTTACTATTTAATGAGTCGTGGTATTTCTCAAAGAGAAGCG
    GAACGTCTTGTTATACATGGTTTCTTAGATCCAGTAGTACGTGAATTACCTATCGAAGAC
    GTTAAACGTCAATTGAGAGAAGTAATTGAACGCAAAGTTTCTAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1308

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_000802
  • symbol: SufD
  • description: Fe-S cluster assembly protein SufD
  • length: 435
  • theoretical pI: 5.21182
  • theoretical MW: 48562.3
  • GRAVY: -0.353103

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other FeS assembly protein SufD (TIGR01981; HMM-score: 239.2)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other FeS assembly protein SufB (TIGR01980; HMM-score: 232.6)
    and 1 more
    RNA polymerase sigma-70 factor, Bacteroides expansion family 1 (TIGR02985; HMM-score: 12.6)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    no clan defined SUFBD_core; SUF system FeS cluster assembly, SufBD core domain (PF01458; HMM-score: 218.2)
    and 2 more
    SufBD_N; SufBD protein N-terminal region (PF19295; HMM-score: 64.1)
    HTH (CL0123) HTH_ABP1_N; Fission yeast centromere protein N-terminal domain (PF18107; HMM-score: 14.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.8243
    • Cytoplasmic Membrane Score: 0.0956
    • Cell wall & surface Score: 0.0081
    • Extracellular Score: 0.072
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003071
    • TAT(Tat/SPI): 0.000205
    • LIPO(Sec/SPII): 0.000454
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MTTDILNISEEQLVDYSKAHNEPSWMTELRKKALKLTETLEMPKPDKTKLRKWDFESFKQHDVKGDVYQSLSQLPESVREIIDVDHSKNLVIQHNNTIAYTQVDDNASKDGVIVEGLADALMNHSDLVQKYFMKDAVTVDEHRITALHTALVNGGVFVYVPKNVVVEHPVQYVVLHDDENASFYNHVIIVTEESAEVTYVENYLSNASGEGNQLNIISEVIAGANSNITYGSVDYMDKGFTGHIIRRGITEADASINWALGLMNEGSQIIDNTTNLFGDRSTSSLKSVVVGTGEQKINLTSKIVQYGKETDGYILKHGVMKEHASSVFNGIGYIKHGGTKSIANQESRVLMLSEHARGDANPILLIDEDDVQAGHAASVGRVDPDQLYYLMSRGISQREAERLVIHGFLDPVVRELPIEDVKRQLREVIERKVSK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: PerR (repression) regulon, SigB (activation) regulon
    PerR(TF)important in Oxidative stress response;  regulation predicted or transferred from N315 and NCTC 8325  [2]
    SigB(sigma factor)controlling a large regulon involved in stress/starvation response and adaptation;  regulation predicted or transferred from N315 and NCTC 8325  [3] [4] [5]   other strains

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)
  2. ↑ Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)
  3. ↑ Markus Bischoff, Paul Dunman, Jan Kormanec, Daphne Macapagal, Ellen Murphy, William Mounts, Brigitte Berger-Bächi, Steven Projan
    Microarray-based analysis of the Staphylococcus aureus sigmaB regulon.
    J Bacteriol: 2004, 186(13);4085-99
    [PubMed:15205410] [WorldCat.org] [DOI] (P p)
  4. ↑ Jan Pané-Farré, Beate Jonas, Konrad Förstner, Susanne Engelmann, Michael Hecker
    The sigmaB regulon in Staphylococcus aureus and its regulation.
    Int J Med Microbiol: 2006, 296(4-5);237-58
    [PubMed:16644280] [WorldCat.org] [DOI] (P p)
  5. ↑ Bettina Schulthess, Dominik A Bloes, Patrice François, Myriam Girard, Jacques Schrenzel, Markus Bischoff, Brigitte Berger-Bächi
    The σB-dependent yabJ-spoVG operon is involved in the regulation of extracellular nuclease, lipase, and protease expression in Staphylococcus aureus.
    J Bacteriol: 2011, 193(18);4954-62
    [PubMed:21725011] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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