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NCBI: 10-JUN-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus COL
- locus tag: SACOL1001 [new locus tag: SACOL_RS05115 ]
- pan locus tag?: SAUPAN003163000
- symbol: trpS
- pan gene symbol?: trpS
- synonym:
- product: tryptophanyl-tRNA synthetase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SACOL1001 [new locus tag: SACOL_RS05115 ]
- symbol: trpS
- product: tryptophanyl-tRNA synthetase
- replicon: chromosome
- strand: -
- coordinates: 1009158..1010147
- length: 990
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3236281 NCBI
- RefSeq: YP_185869 NCBI
- BioCyc: see SACOL_RS05115
- MicrobesOnline: 912470 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
- 1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961ATGGAGACATTATTTTCAGGCATCCAACCTAGTGGAATTCCTACTATTGGAAATTATATT
GGCGCACTAAAACAATTTGTTGATGTGCAAAATGACTATGATTGTTATTTCTGTATCGTA
GATCAACATGCAATTACAATGCCACAAGATCGTTTAAAATTACGTAAACAGACCAGACAA
TTAGCAGCGATTTATTTAGCTTCTGGTATAGATCCAGACAAAGCAACATTGTTCATACAA
TCTGAAGTCCCTGCACACGTACAAGCAGGATGGATGTTAACTACGATTGCTTCTGTTGGA
GAATTAGAGCGTATGACGCAATACAAAGATAAAGCTCAGAAAGCAGTTGAAGGTATACCT
GCTGGTCTATTAACATATCCACCTTTAATGGCAGCTGATATTGTTCTTTACAATACTAAT
ATCGTTCCAGTTGGAGATGACCAAAAGCAGCATATCGAATTGACTCGTAACCTTGTAGAT
AGATTTAATAGTCGCTATAATGATGTGCTTGTGAAACCTGAAATTCGTATGCCTAAAGTT
GGTGGACGTGTCATGAGTTTACAAGACCCAACAAGAAAAATGAGTAAGAGTGATGATAAT
GCTAAAAACTTCATTTCATTATTAGACGAGCCGAATGTTGCAGCTAAAAAAATTAAAAGC
GCAGTAACTGATTCAGATGGTATTATTAAATTTGATCGTGACAACAAGCCAGGTATAACA
AATTTAATTTCAATATACGCTGGATTAACAGACATGCCAATTAAAGATATTGAGGCAAAA
TATGAGGGCGAAGGTTATGGTAAATTTAAAGGTGACCTTGCTGAAATAGTTAAAGCATTT
TTAGTAGAATTCCAAGAAAAATACGAAAGTTTCTATAACTCAGATAAACTTGATGATATT
TTAGATCAAGGTAGAGATAAAGCACACAAAGTTTCATTTAAAACTGTCAAAAAAATGGAA
AAAGCGATGGGTTTAGGACGTAAGAGATAA60
120
180
240
300
360
420
480
540
600
660
720
780
840
900
960
990
⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SACOL1001 [new locus tag: SACOL_RS05115 ]
- symbol: TrpS
- description: tryptophanyl-tRNA synthetase
- length: 329
- theoretical pI: 8.14756
- theoretical MW: 36909.2
- GRAVY: -0.387538
⊟Function[edit | edit source]
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- METLFSGIQPSGIPTIGNYIGALKQFVDVQNDYDCYFCIVDQHAITMPQDRLKLRKQTRQLAAIYLASGIDPDKATLFIQSEVPAHVQAGWMLTTIASVGELERMTQYKDKAQKAVEGIPAGLLTYPPLMAADIVLYNTNIVPVGDDQKQHIELTRNLVDRFNSRYNDVLVKPEIRMPKVGGRVMSLQDPTRKMSKSDDNAKNFISLLDEPNVAAKKIKSAVTDSDGIIKFDRDNKPGITNLISIYAGLTDMPIKDIEAKYEGEGYGKFKGDLAEIVKAFLVEFQEKYESFYNSDKLDDILDQGRDKAHKVSFKTVKKMEKAMGLGRKR
⊟Experimental data[edit | edit source]
- experimentally validated: PeptideAtlas
- protein localization: Cytoplasmic [1] [2] [3]
- quantitative data / protein copy number per cell: 1084 [4]
- ⊟⊟interaction partners:
SACOL1587 (efp) elongation factor P [5] (data from MRSA252) SACOL2092 (murAA) UDP-N-acetylglucosamine 1-carboxyvinyltransferase [5] (data from MRSA252) SACOL0841 (pgm) phosphoglyceromutase [5] (data from MRSA252) SACOL1011 (ppnK) inorganic polyphosphate/ATP-NAD kinase [5] (data from MRSA252) SACOL1091 (ptsH) phosphocarrier protein HPr [5] (data from MRSA252) SACOL0731 LysR family transcriptional regulator [5] (data from MRSA252) SACOL1437 CSD family cold shock protein [5] (data from MRSA252)
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: no polycistronic organisation predicted
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: 22.29 h [6]
⊞Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊞Other Information[edit | edit source]
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
PLoS One: 2009, 4(12);e8176
[PubMed:19997597] [WorldCat.org] [DOI] (I e) - ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
J Proteome Res: 2011, 10(4);1657-66
[PubMed:21323324] [WorldCat.org] [DOI] (I p) - ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
The Staphylococcus aureus proteome.
Int J Med Microbiol: 2014, 304(2);110-20
[PubMed:24439828] [WorldCat.org] [DOI] (I p) - ↑ Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
Sci Rep: 2016, 6;28172
[PubMed:27344979] [WorldCat.org] [DOI] (I e) - ↑ Jump up to: 5.0 5.1 5.2 5.3 5.4 5.5 5.6 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
J Proteome Res: 2011, 10(3);1139-50
[PubMed:21166474] [WorldCat.org] [DOI] (I p) - ↑ Stephan Michalik, Jörg Bernhardt, Andreas Otto, Martin Moche, Dörte Becher, Hanna Meyer, Michael Lalk, Claudia Schurmann, Rabea Schlüter, Holger Kock, Ulf Gerth, Michael Hecker
Life and death of proteins: a case study of glucose-starved Staphylococcus aureus.
Mol Cell Proteomics: 2012, 11(9);558-70
[PubMed:22556279] [WorldCat.org] [DOI] (I p)
⊟Relevant publications[edit | edit source]
Suzanne E Dale, Amanda Doherty-Kirby, Gilles Lajoie, David E Heinrichs
Role of siderophore biosynthesis in virulence of Staphylococcus aureus: identification and characterization of genes involved in production of a siderophore.
Infect Immun: 2004, 72(1);29-37
[PubMed:14688077] [WorldCat.org] [DOI] (P p)
NCBI: 10-JUN-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus COL
- locus tag: SACOL1001 [new locus tag: SACOL_RS05115 ]
- pan locus tag?: SAUPAN003163000
- symbol: trpS
- pan gene symbol?: trpS
- synonym:
- product: tryptophanyl-tRNA synthetase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SACOL1001 [new locus tag: SACOL_RS05115 ]
- symbol: trpS
- product: tryptophanyl-tRNA synthetase
- replicon: chromosome
- strand: -
- coordinates: 1009158..1010147
- length: 990
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3236281 NCBI
- RefSeq: YP_185869 NCBI
- BioCyc: see SACOL_RS05115
- MicrobesOnline: 912470 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit
]
⊟DNA sequence[edit | edit source]
- 1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961ATGGAGACATTATTTTCAGGCATCCAACCTAGTGGAATTCCTACTATTGGAAATTATATT
GGCGCACTAAAACAATTTGTTGATGTGCAAAATGACTATGATTGTTATTTCTGTATCGTA
GATCAACATGCAATTACAATGCCACAAGATCGTTTAAAATTACGTAAACAGACCAGACAA
TTAGCAGCGATTTATTTAGCTTCTGGTATAGATCCAGACAAAGCAACATTGTTCATACAA
TCTGAAGTCCCTGCACACGTACAAGCAGGATGGATGTTAACTACGATTGCTTCTGTTGGA
GAATTAGAGCGTATGACGCAATACAAAGATAAAGCTCAGAAAGCAGTTGAAGGTATACCT
GCTGGTCTATTAACATATCCACCTTTAATGGCAGCTGATATTGTTCTTTACAATACTAAT
ATCGTTCCAGTTGGAGATGACCAAAAGCAGCATATCGAATTGACTCGTAACCTTGTAGAT
AGATTTAATAGTCGCTATAATGATGTGCTTGTGAAACCTGAAATTCGTATGCCTAAAGTT
GGTGGACGTGTCATGAGTTTACAAGACCCAACAAGAAAAATGAGTAAGAGTGATGATAAT
GCTAAAAACTTCATTTCATTATTAGACGAGCCGAATGTTGCAGCTAAAAAAATTAAAAGC
GCAGTAACTGATTCAGATGGTATTATTAAATTTGATCGTGACAACAAGCCAGGTATAACA
AATTTAATTTCAATATACGCTGGATTAACAGACATGCCAATTAAAGATATTGAGGCAAAA
TATGAGGGCGAAGGTTATGGTAAATTTAAAGGTGACCTTGCTGAAATAGTTAAAGCATTT
TTAGTAGAATTCCAAGAAAAATACGAAAGTTTCTATAACTCAGATAAACTTGATGATATT
TTAGATCAAGGTAGAGATAAAGCACACAAAGTTTCATTTAAAACTGTCAAAAAAATGGAA
AAAGCGATGGGTTTAGGACGTAAGAGATAA60
120
180
240
300
360
420
480
540
600
660
720
780
840
900
960
990
⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SACOL1001 [new locus tag: SACOL_RS05115 ]
- symbol: TrpS
- description: tryptophanyl-tRNA synthetase
- length: 329
- theoretical pI: 8.14756
- theoretical MW: 36909.2
- GRAVY: -0.387538
⊟Function[edit | edit source]
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- METLFSGIQPSGIPTIGNYIGALKQFVDVQNDYDCYFCIVDQHAITMPQDRLKLRKQTRQLAAIYLASGIDPDKATLFIQSEVPAHVQAGWMLTTIASVGELERMTQYKDKAQKAVEGIPAGLLTYPPLMAADIVLYNTNIVPVGDDQKQHIELTRNLVDRFNSRYNDVLVKPEIRMPKVGGRVMSLQDPTRKMSKSDDNAKNFISLLDEPNVAAKKIKSAVTDSDGIIKFDRDNKPGITNLISIYAGLTDMPIKDIEAKYEGEGYGKFKGDLAEIVKAFLVEFQEKYESFYNSDKLDDILDQGRDKAHKVSFKTVKKMEKAMGLGRKR
⊟Experimental data[edit | edit source]
- experimentally validated: PeptideAtlas
- protein localization: Cytoplasmic [1] [2] [3]
- quantitative data / protein copy number per cell: 1084 [4]
- ⊟interaction partners:
SACOL1587 (efp) elongation factor P [5] (data from MRSA252) SACOL2092 (murAA) UDP-N-acetylglucosamine 1-carboxyvinyltransferase [5] (data from MRSA252) SACOL0841 (pgm) phosphoglyceromutase [5] (data from MRSA252) SACOL1011 (ppnK) inorganic polyphosphate/ATP-NAD kinase [5] (data from MRSA252) SACOL1091 (ptsH) phosphocarrier protein HPr [5] (data from MRSA252) SACOL0731 LysR family transcriptional regulator [5] (data from MRSA252) SACOL1437 CSD family cold shock protein [5] (data from MRSA252)
- ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
PLoS One: 2009, 4(12);e8176
[PubMed:19997597] [WorldCat.org] [DOI] (I e) - ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
J Proteome Res: 2011, 10(4);1657-66
[PubMed:21323324] [WorldCat.org] [DOI] (I p) - ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
The Staphylococcus aureus proteome.
Int J Med Microbiol: 2014, 304(2);110-20
[PubMed:24439828] [WorldCat.org] [DOI] (I p) - ↑ Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
Sci Rep: 2016, 6;28172
[PubMed:27344979] [WorldCat.org] [DOI] (I e) - ↑ 5.0 5.1 5.2 5.3 5.4 5.5 5.6 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
J Proteome Res: 2011, 10(3);1139-50
[PubMed:21166474] [WorldCat.org] [DOI] (I p)
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: no polycistronic organisation predicted
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: 22.29 h [1]
- ↑ Stephan Michalik, Jörg Bernhardt, Andreas Otto, Martin Moche, Dörte Becher, Hanna Meyer, Michael Lalk, Claudia Schurmann, Rabea Schlüter, Holger Kock, Ulf Gerth, Michael Hecker
Life and death of proteins: a case study of glucose-starved Staphylococcus aureus.
Mol Cell Proteomics: 2012, 11(9);558-70
[PubMed:22556279] [WorldCat.org] [DOI] (I p)
⊞Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊞Other Information[edit | edit source]
⊟Literature[edit | edit source]
⊟References[edit | edit source]
⊟Relevant publications[edit | edit source]
Suzanne E Dale, Amanda Doherty-Kirby, Gilles Lajoie, David E Heinrichs
Role of siderophore biosynthesis in virulence of Staphylococcus aureus: identification and characterization of genes involved in production of a siderophore.
Infect Immun: 2004, 72(1);29-37
[PubMed:14688077] [WorldCat.org] [DOI] (P p)