From AureoWiki
Jump to navigation Jump to search

NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS05115 [old locus tag: SACOL1001 ]
  • pan locus tag?: SAUPAN003163000
  • symbol: SACOL_RS05115
  • pan gene symbol?: trpS
  • synonym:
  • product: tryptophan--tRNA ligase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL_RS05115 [old locus tag: SACOL1001 ]
  • symbol: SACOL_RS05115
  • product: tryptophan--tRNA ligase
  • replicon: chromosome
  • strand: -
  • coordinates: 1009158..1010147
  • length: 990
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002951 (1009158..1010147) NCBI
  • BioCyc: SACOL_RS05115 BioCyc
  • MicrobesOnline: see SACOL1001

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGGAGACATTATTTTCAGGCATCCAACCTAGTGGAATTCCTACTATTGGAAATTATATT
    GGCGCACTAAAACAATTTGTTGATGTGCAAAATGACTATGATTGTTATTTCTGTATCGTA
    GATCAACATGCAATTACAATGCCACAAGATCGTTTAAAATTACGTAAACAGACCAGACAA
    TTAGCAGCGATTTATTTAGCTTCTGGTATAGATCCAGACAAAGCAACATTGTTCATACAA
    TCTGAAGTCCCTGCACACGTACAAGCAGGATGGATGTTAACTACGATTGCTTCTGTTGGA
    GAATTAGAGCGTATGACGCAATACAAAGATAAAGCTCAGAAAGCAGTTGAAGGTATACCT
    GCTGGTCTATTAACATATCCACCTTTAATGGCAGCTGATATTGTTCTTTACAATACTAAT
    ATCGTTCCAGTTGGAGATGACCAAAAGCAGCATATCGAATTGACTCGTAACCTTGTAGAT
    AGATTTAATAGTCGCTATAATGATGTGCTTGTGAAACCTGAAATTCGTATGCCTAAAGTT
    GGTGGACGTGTCATGAGTTTACAAGACCCAACAAGAAAAATGAGTAAGAGTGATGATAAT
    GCTAAAAACTTCATTTCATTATTAGACGAGCCGAATGTTGCAGCTAAAAAAATTAAAAGC
    GCAGTAACTGATTCAGATGGTATTATTAAATTTGATCGTGACAACAAGCCAGGTATAACA
    AATTTAATTTCAATATACGCTGGATTAACAGACATGCCAATTAAAGATATTGAGGCAAAA
    TATGAGGGCGAAGGTTATGGTAAATTTAAAGGTGACCTTGCTGAAATAGTTAAAGCATTT
    TTAGTAGAATTCCAAGAAAAATACGAAAGTTTCTATAACTCAGATAAACTTGATGATATT
    TTAGATCAAGGTAGAGATAAAGCACACAAAGTTTCATTTAAAACTGTCAAAAAAATGGAA
    AAAGCGATGGGTTTAGGACGTAAGAGATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    990

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL_RS05115 [old locus tag: SACOL1001 ]
  • symbol: SACOL_RS05115
  • description: tryptophan--tRNA ligase
  • length: 329
  • theoretical pI: 8.14756
  • theoretical MW: 36909.2
  • GRAVY: -0.387538

⊟Function[edit | edit source]

  • ⊞reaction:
    EC 6.1.1.2?  ExPASy
    Tryptophan--tRNA ligase ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp)
  • TIGRFAM:
    Genetic information processing Protein synthesis tRNA aminoacylation tryptophan--tRNA ligase (TIGR00233; EC 6.1.1.2; HMM-score: 362.5)
  • TheSEED: see SACOL1001
  • PFAM:
    HUP (CL0039) tRNA-synt_1b; tRNA synthetases class I (W and Y) (PF00579; HMM-score: 260.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 10
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9972
    • Cytoplasmic Membrane Score: 0
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.0027
  • LocateP:
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.007356
    • TAT(Tat/SPI): 0.000216
    • LIPO(Sec/SPII): 0.001212
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • METLFSGIQPSGIPTIGNYIGALKQFVDVQNDYDCYFCIVDQHAITMPQDRLKLRKQTRQLAAIYLASGIDPDKATLFIQSEVPAHVQAGWMLTTIASVGELERMTQYKDKAQKAVEGIPAGLLTYPPLMAADIVLYNTNIVPVGDDQKQHIELTRNLVDRFNSRYNDVLVKPEIRMPKVGGRVMSLQDPTRKMSKSDDNAKNFISLLDEPNVAAKKIKSAVTDSDGIIKFDRDNKPGITNLISIYAGLTDMPIKDIEAKYEGEGYGKFKGDLAEIVKAFLVEFQEKYESFYNSDKLDDILDQGRDKAHKVSFKTVKKMEKAMGLGRKR

⊟Experimental data[edit | edit source]

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Jump up to: 1.0 1.1 1.2 1.3 1.4 1.5 1.6 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=SACOL_RS05115&oldid=89024"