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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1492 [new locus tag: NWMN_RS08400 ]
  • pan locus tag?: SAUPAN004176000
  • symbol: comEA
  • pan gene symbol?: comEA
  • synonym:
  • product: competence protein ComEA

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_1492 [new locus tag: NWMN_RS08400 ]
  • symbol: comEA
  • product: competence protein ComEA
  • replicon: chromosome
  • strand: -
  • coordinates: 1660991..1661668
  • length: 678
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    TTGTATCAATTTTTATTACGCTATAAAGATTTTTTAACTCAATGGAAGTTATATATTCTA
    AGTGCTGTTGTTTTAATTATGGTATTAATTGGTTTTATATTCTGGAGACAAGATGATTAT
    ACTTCAAGAAATTTTGAAAATAAAGATACTGCTCTGAAACAAAGCACTAGTGAAAATAGT
    AGTTTGTCCAAAGTAGAAGATGTCCAGGTCAAAGATGGAGATAATTCCAAAAATAAGGGT
    CCTGTATATGTCGATGTAAAAGGTGCTGTTAAACATCCTAATGTTTATAAAATGACATCT
    AAGGATAGAGTAGTTGATTTACTTGATAAAGCACAATTATTGGAGGATGCAGATGTAAGT
    CAAATTAATTTGTCTGAAAAATTAACAGATCAAAAAATGATTTTCATACCTCATAAAGGA
    CAAAAGAATGTTGAACCACAAATTGAAGTAAACAGTGTGCACGAAAAAAATGGGAACACA
    AATAATACTAAAGTAAATTTAAATACGGCATCTGTATCAGAATTGATGTCTGTTCCTGGA
    GTTGGGCAAGCTAAAGCTAATGCAATTGTTGAATATCGCAACCAACAAGGTGCATTTCAA
    GAAATTGACGATTTGAAAAAAGTAAAAGGTTTTGGAAGTAAAACTTTTGATAAACTGAAA
    TCTTATTTCACGACATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    678

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_1492 [new locus tag: NWMN_RS08400 ]
  • symbol: ComEA
  • description: competence protein ComEA
  • length: 225
  • theoretical pI: 9.36125
  • theoretical MW: 25537.9
  • GRAVY: -0.521333

⊟Function[edit | edit source]

  • ⊞TIGRFAM:
    Cellular processes Cellular processes DNA transformation comEA protein (TIGR01259; HMM-score: 116.3)
    and 4 more
    Cellular processes Cellular processes DNA transformation competence protein ComEA helix-hairpin-helix repeat region (TIGR00426; HMM-score: 74.2)
    Unknown function Enzymes of unknown specificity putative DNA modification/repair radical SAM protein (TIGR03916; HMM-score: 20.7)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair Holliday junction DNA helicase RuvA (TIGR00084; EC 3.6.4.12; HMM-score: 13.3)
    Metabolism Transport and binding proteins Nucleosides, purines and pyrimidines nucleoside Transporter (TIGR00939; HMM-score: 12.3)
  • ⊞TheSEED  :
    • Late competence protein ComEA, DNA receptor
    DNA Metabolism DNA uptake, competence Gram Positive Competence  Late competence protein ComEA, DNA receptor
    and 1 more
    DNA Metabolism DNA uptake, competence Late competence  Late competence protein ComEA, DNA receptor
  • ⊞PFAM:
    HHH (CL0198) HHH_3; Helix-hairpin-helix motif (PF12836; HMM-score: 89.5)
    and 14 more
    HHH; Helix-hairpin-helix motif (PF00633; HMM-score: 47.7)
    HHH_5; Helix-hairpin-helix domain (PF14520; HMM-score: 33.5)
    T2SSK; Type II secretion system (T2SS), protein K (PF03934; HMM-score: 23.6)
    HHH_2; Helix-hairpin-helix motif (PF12826; HMM-score: 22.2)
    Ubiquitin (CL0072) SLBB; SLBB domain (PF10531; HMM-score: 21.5)
    HHH (CL0198) HHH_6; Helix-hairpin-helix motif (PF14579; HMM-score: 19.2)
    HHH_8; Helix-hairpin-helix domain (PF14716; HMM-score: 16.6)
    PsbU; Photosystem II 12 kDa extrinsic protein (PsbU) (PF06514; HMM-score: 16.3)
    T2SSK_1st; Type II secretion system (T2SS), protein K (PF21687; HMM-score: 15.5)
    H2TH (CL0303) H2TH_5; S13-like H2TH domain (PF22525; HMM-score: 14.6)
    HHH (CL0198) UPF0758_N; UPF0758 N-terminal (PF20582; HMM-score: 14.5)
    RNA_pol_A_CTD; Bacterial RNA polymerase, alpha chain C terminal domain (PF03118; HMM-score: 13.6)
    Ubiquitin (CL0072) SLBB_2; SLBB domain (PF22461; HMM-score: 13.3)
    PH (CL0266) FERM_C_FAK1; FAK1/PYK2, FERM domain C-lobe (PF21477; HMM-score: 12.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.17
    • Cytoplasmic Membrane Score: 9.51
    • Cellwall Score: 0.16
    • Extracellular Score: 0.15
    • Internal Helix: 1
  • ⊞DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.0001
    • Cytoplasmic Membrane Score: 0.9818
    • Cell wall & surface Score: 0.0012
    • Extracellular Score: 0.0169
  • ⊞LocateP: N-terminally anchored (No CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 4
    • Predicted Cleavage Site: No CleavageSite
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004614
    • TAT(Tat/SPI): 0.000377
    • LIPO(Sec/SPII): 0.002017
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MYQFLLRYKDFLTQWKLYILSAVVLIMVLIGFIFWRQDDYTSRNFENKDTALKQSTSENSSLSKVEDVQVKDGDNSKNKGPVYVDVKGAVKHPNVYKMTSKDRVVDLLDKAQLLEDADVSQINLSEKLTDQKMIFIPHKGQKNVEPQIEVNSVHEKNGNTNNTKVNLNTASVSELMSVPGVGQAKANAIVEYRNQQGAFQEIDDLKKVKGFGSKTFDKLKSYFTT

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • data available for N315

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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