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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

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⊟Summary[edit | edit source]

Contents

  • 1 Summary
  • 2 Genome View
  • 3 Gene
    • 3.1 General
    • 3.2 Accession numbers
    • 3.3 Phenotype
    • 3.4 DNA sequence
  • 4 Protein
    • 4.1 General
    • 4.2 Function
    • 4.3 Structure, modifications & cofactors
    • 4.4 Localization
    • 4.5 Accession numbers
    • 4.6 Protein sequence
    • 4.7 Experimental data
  • 5 Expression & Regulation
    • 5.1 Operon
    • 5.2 Regulation
    • 5.3 Transcription pattern
    • 5.4 Protein synthesis (provided by Aureolib)
    • 5.5 Protein stability
  • 6 Biological Material
    • 6.1 Mutants
    • 6.2 Expression vector
    • 6.3 lacZ fusion
    • 6.4 GFP fusion
    • 6.5 two-hybrid system
    • 6.6 FLAG-tag construct
    • 6.7 Antibody
  • 7 Other Information
  • 8 Literature
    • 8.1 References
    • 8.2 Relevant publications
  • organism: Staphylococcus aureus N315
  • locus tag: SA1507 [new locus tag: SA_RS08490 ]
  • pan locus tag?: SAUPAN004306000
  • symbol: dnaI
  • pan gene symbol?: dnaI
  • synonym:
  • product: primosomal protein DnaI

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA1507 [new locus tag: SA_RS08490 ]
  • symbol: dnaI
  • product: primosomal protein DnaI
  • replicon: chromosome
  • strand: -
  • coordinates: 1716373..1717293
  • length: 921
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

  • Gene ID: 1124352 NCBI
  • RefSeq: NP_374795 NCBI
  • BioCyc: see SA_RS08490
  • MicrobesOnline: 103821 MicrobesOnline

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGAAGCAATTTAAAAGTATAATTAACACGTCGCAGGACTTTGAAAAAAGAATAGAAAAG
    ATAAAAAAAGAAGTAATCAATGACCCAGATGTTAAGCAATTTTTGGAAGCGCATCGAGCT
    GAATTAACGAATGCTATGATTGATGAAGACTTAAATGTGTTACAAGAGTATAAAGATCAA
    CAAAAACATTATGACGGTCATAAATTTGCTGATTGTCCAAATTTCGTAAAGGGGCATGTG
    CCTGAGTTATATGTTGATAATAACCGAATTAAAATACGCTATTTACAATGCCCATGTAAA
    ATCAAGTACGACGAAGAACGCTTTGAAGCTGAGCTAATTACATCTCATCATATGCAACGA
    GATACTTTAAATGCCAAATTGAAAGATATTTATATGAATCATCGAGACCGTCTTGATGTA
    GCTATGGCAGCAGATGATATTTGTACAGCAATAACTAATGGGGAACAAGTGAAAGGCCTT
    TACCTTTATGGTCCATTTGGGACAGGTAAATCTTTTATTCTAGGTGCAATTGCGAATCAG
    CTCAAATCTAAGAAGGTACGTTCGACAATTATTTATTTACCGGAATTTATTAGAACATTA
    AAAGGTGGCTTTAAAGATGGTTCTTTTGAAAAGAAATTACATCGCGTAAGAGAAGCAAAC
    ATTTTAATGCTTGATGATATTGGGGCTGAAGAAGTGACTCCATGGGTGAGAGATGAGGTA
    ATTGGACCTTTGCTACACTATCGAATGGTTCATGAATTACCAACATTCTTTAGTTCTAAT
    TTTGACTATAGTGAATTGGAACATCATTTAGCGATGACTCGTGATGGTGAAGAGAAGACT
    AAAGCAGCACGTATTATTGAACGTGTCAAATCTTTGTCAACACCATACTTTTTATCAGGA
    GAAAATTTCAGAAACAATTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    921

⊟Protein[edit | edit source]

  • 5HE8
    PDB
  • 5HE9
    PDB
Protein Data Bank: 5HE8
Protein Data Bank: 5HE9

⊟General[edit | edit source]

  • locus tag: SA1507 [new locus tag: SA_RS08490 ]
  • symbol: DnaI
  • description: primosomal protein DnaI
  • length: 306
  • theoretical pI: 7.15118
  • theoretical MW: 35635.5
  • GRAVY: -0.589543

⊟Function[edit | edit source]

  • ⊞TIGRFAM:
    Genetic information processing DNA metabolism DNA replication, recombination, and repair chromosomal replication initiator protein DnaA (TIGR00362; HMM-score: 38.3)
    and 6 more
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DnaA regulatory inactivator Hda (TIGR03420; HMM-score: 21)
    AAA family ATPase, CDC48 subfamily (TIGR01243; HMM-score: 17.9)
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides proteasome ATPase (TIGR03689; EC 3.6.4.8; HMM-score: 17.3)
    26S proteasome subunit P45 family (TIGR01242; HMM-score: 15.5)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair orc1/cdc6 family replication initiation protein (TIGR02928; HMM-score: 13.7)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair Holliday junction DNA helicase RuvB (TIGR00635; EC 3.6.4.12; HMM-score: 11.5)
  • TheSEED  :
    • Replicative DNA helicase loader DnaC (=BsDnaI)
    DNA Metabolism DNA replication DNA-replication  Helicase loader DnaI
  • ⊞PFAM:
    no clan defined DnaI_N; Primosomal protein DnaI N-terminus (PF07319; HMM-score: 91.5)
    and 20 more
    P-loop_NTPase (CL0023) Bac_DnaA; Bacterial DnaA ATPAse domain (PF00308; HMM-score: 32.1)
    IstB_IS21; IstB-like ATP binding protein (PF01695; HMM-score: 28.9)
    AAA; ATPase family associated with various cellular activities (AAA) (PF00004; HMM-score: 24.2)
    AFG1_ATPase; AFG1-like ATPase (PF03969; HMM-score: 21)
    AAA_16; AAA ATPase domain (PF13191; HMM-score: 19.5)
    DUF5906; Family of unknown function (DUF5906) (PF19263; HMM-score: 18.1)
    PhoH; PhoH-like protein (PF02562; HMM-score: 16.1)
    AAA_22; AAA domain (PF13401; HMM-score: 15.5)
    PIF1; PIF1-like helicase (PF05970; HMM-score: 15.3)
    NPHP3_N; Nephrocystin 3, N-terminal (PF24883; HMM-score: 14.9)
    KAP_NTPase; KAP family P-loop domain (PF07693; HMM-score: 14)
    RNA_helicase; RNA helicase (PF00910; HMM-score: 13.5)
    ATPase_2; ATPase domain predominantly from Archaea (PF01637; HMM-score: 13.5)
    TsaE; Threonylcarbamoyl adenosine biosynthesis protein TsaE (PF02367; HMM-score: 13.2)
    ResIII; Type III restriction enzyme, res subunit (PF04851; HMM-score: 13.1)
    AAA_5; AAA domain (dynein-related subfamily) (PF07728; HMM-score: 12.8)
    AAA_7; P-loop containing dynein motor region (PF12775; HMM-score: 12.2)
    DUF815; Protein of unknown function (DUF815) (PF05673; HMM-score: 12.1)
    Cytidylate_kin; Cytidylate kinase (PF02224; HMM-score: 11.9)
    T3SS-hook (CL0424) Flg_hook; Flagellar hook-length control protein FliK (PF02120; HMM-score: 11.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9595
    • Cytoplasmic Membrane Score: 0.0305
    • Cell wall & surface Score: 0.0011
    • Extracellular Score: 0.0089
  • ⊞LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003359
    • TAT(Tat/SPI): 0.000484
    • LIPO(Sec/SPII): 0.000445
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI: 15927262 NCBI
  • RefSeq: NP_374795 NCBI
  • UniProt: A0A0H3JMY8 UniProt

⊟Protein sequence[edit | edit source]

  • MKQFKSIINTSQDFEKRIEKIKKEVINDPDVKQFLEAHRAELTNAMIDEDLNVLQEYKDQQKHYDGHKFADCPNFVKGHVPELYVDNNRIKIRYLQCPCKIKYDEERFEAELITSHHMQRDTLNAKLKDIYMNHRDRLDVAMAADDICTAITNGEQVKGLYLYGPFGTGKSFILGAIANQLKSKKVRSTIIYLPEFIRTLKGGFKDGSFEKKLHRVREANILMLDDIGAEEVTPWVRDEVIGPLLHYRMVHELPTFFSSNFDYSELEHHLAMTRDGEEKTKAARIIERVKSLSTPYFLSGENFRNN

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

  • MicrobesOnline: dnaI < dnaB < nrdR

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

  • S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

  • Aureolib: no data available

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=SA1507&oldid=95018"
  • This page was last edited on 11 March 2016, at 10:18.
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