PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_1630 [new locus tag: SAUSA300_RS08895 ]
• pan locus tag^?: SAUPAN004306000
• symbol: dnaI
• pan gene symbol^?: dnaI
• synonym:
• product: primosomal protein DnaI

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_1630 [new locus tag: SAUSA300_RS08895 ]
• symbol: dnaI
• product: primosomal protein DnaI
• replicon: chromosome
• strand: -
• coordinates: 1784903..1785823
• length: 921
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3914456 NCBI
• RefSeq: YP_494325 NCBI
• BioCyc: see SAUSA300_RS08895
• MicrobesOnline: 1293145 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGAAGCAATTTAAAAGTATAATTAACACGTCGCAGGACTTTGAAAAAAGAATAGAAAAG
  ATAAAAAAAGAAGTAATCAATGACCCAGATGTTAAGCAATTTTTGGAAGCGCATCGAGCT
  GAATTAACGAATGCTATGATTGATGAAGACTTAAATGTGTTACAAGAGTATAAAGATCAA
  CAAAAACATTATGACGGTCATAAATTTGCTGATTGTCCAAATTTCGTAAAGGGGCATGTG
  CCTGAGTTATATGTTGATAATAACCGAATTAAAATACGCTATTTACAATGCCCATGTAAA
  ATCAAGTACGACGAAGAACGCTTTGAAGCTGAGCTAATTACATCTCATCATATGCAACGA
  GATACTTTAAATGCCAAATTGAAAGATATTTATATGAATCATCGAGACCGTCTTGATGTA
  GCTATGGCAGCAGATGATATTTGTACAGCAATAACTAATGGGGAACAAGTGAAAGGCCTT
  TACCTTTATGGTCCATTTGGGACAGGTAAATCTTTTATTCTAGGTGCAATTGCGAATCAG
  CTCAAATCTAAGAAGGTACGTTCGACAATTATTTATTTACCGGAATTTATTAGAACATTA
  AAAGGTGGCTTTAAAGATGGTTCTTTTGAAAAGAAATTACATCGCGTAAGAGAAGCAAAC
  ATTTTAATGCTTGATGATATTGGGGCTGAAGAAGTGACTCCATGGGTGAGAGATGAGGTA
  ATTGGACCTTTGCTACATTATCGAATGGTTCATGAATTACCAACATTCTTTAGTTCTAAT
  TTTGACTATAGTGAATTGGAACATCATTTAGCGATGACTCGTGATGGTGAAGAGAAGACT
  AAAGCAGCACGTATTATTGAACGTGTCAAATCTTTGTCAACACCATACTTTTTATCAGGA
  GAAAATTTCAGAAACAATTGA
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⊟Protein

• 5HE8
  PDB
• 5HE9
  PDB

Protein Data Bank: 5HE8

Protein Data Bank: 5HE9

⊟General

• locus tag: SAUSA300_1630 [new locus tag: SAUSA300_RS08895 ]
• symbol: DnaI
• description: primosomal protein DnaI
• length: 306
• theoretical pI: 7.15118
• theoretical MW: 35635.5
• GRAVY: -0.589543

⊟Function

• TIGRFAM:
  Genetic information processing DNA metabolism DNA replication, recombination, and repair chromosomal replication initiator protein DnaA (TIGR00362; HMM-score: 38.3)
  and 6 more
  Genetic information processing DNA metabolism DNA replication, recombination, and repair DnaA regulatory inactivator Hda (TIGR03420; HMM-score: 21)
  AAA family ATPase, CDC48 subfamily (TIGR01243; HMM-score: 17.9)
  Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides proteasome ATPase (TIGR03689; EC 3.6.4.8; HMM-score: 17.3)
  26S proteasome subunit P45 family (TIGR01242; HMM-score: 15.5)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair orc1/cdc6 family replication initiation protein (TIGR02928; HMM-score: 13.7)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair Holliday junction DNA helicase RuvB (TIGR00635; EC 3.6.4.12; HMM-score: 11.5)
• TheSEED  :
  • Replicative DNA helicase loader DnaC (=BsDnaI)
  DNA Metabolism DNA replication DNA-replication  Helicase loader DnaI
• PFAM:
  no clan defined DnaI_N; Primosomal protein DnaI N-terminus (PF07319; HMM-score: 94.4)
  and 16 more
  P-loop_NTPase (CL0023) IstB_IS21; IstB-like ATP binding protein (PF01695; HMM-score: 36.8)
  Bac_DnaA; Bacterial dnaA protein (PF00308; HMM-score: 31.3)
  AAA; ATPase family associated with various cellular activities (AAA) (PF00004; HMM-score: 24.5)
  AFG1_ATPase; AFG1-like ATPase (PF03969; HMM-score: 21.6)
  PIF1; PIF1-like helicase (PF05970; HMM-score: 18.6)
  AAA_16; AAA ATPase domain (PF13191; HMM-score: 17)
  PhoH; PhoH-like protein (PF02562; HMM-score: 16.2)
  TsaE; Threonylcarbamoyl adenosine biosynthesis protein TsaE (PF02367; HMM-score: 14.3)
  RNA_helicase; RNA helicase (PF00910; HMM-score: 13.8)
  KAP_NTPase; KAP family P-loop domain (PF07693; HMM-score: 13.3)
  AAA_22; AAA domain (PF13401; HMM-score: 13.2)
  AAA_5; AAA domain (dynein-related subfamily) (PF07728; HMM-score: 12.3)
  ATPase_2; ATPase domain predominantly from Archaea (PF01637; HMM-score: 12.2)
  AAA_30; AAA domain (PF13604; HMM-score: 12.1)
  DUF815; Protein of unknown function (DUF815) (PF05673; HMM-score: 11.9)
  T3SS-hook (CL0424) Flg_hook; Flagellar hook-length control protein FliK (PF02120; HMM-score: 11.8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.003359
  • TAT(Tat/SPI): 0.000484
  • LIPO(Sec/SPII): 0.000445
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87161691 NCBI
• RefSeq: YP_494325 NCBI
• UniProt: A0A0H2XJA9 UniProt

⊟Protein sequence

• MKQFKSIINTSQDFEKRIEKIKKEVINDPDVKQFLEAHRAELTNAMIDEDLNVLQEYKDQQKHYDGHKFADCPNFVKGHVPELYVDNNRIKIRYLQCPCKIKYDEERFEAELITSHHMQRDTLNAKLKDIYMNHRDRLDVAMAADDICTAITNGEQVKGLYLYGPFGTGKSFILGAIANQLKSKKVRSTIIYLPEFIRTLKGGFKDGSFEKKLHRVREANILMLDDIGAEEVTPWVRDEVIGPLLHYRMVHELPTFFSSNFDYSELEHHLAMTRDGEEKTKAARIIERVKSLSTPYFLSGENFRNN

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: dnaI < SAUSA300_1631 < nrdR

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications