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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_RS08895 [old locus tag: SAUSA300_1630 ]
  • pan locus tag?: SAUPAN004306000
  • symbol: SAUSA300_RS08895
  • pan gene symbol?: dnaI
  • synonym:
  • product: primosomal protein DnaI

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_RS08895 [old locus tag: SAUSA300_1630 ]
  • symbol: SAUSA300_RS08895
  • product: primosomal protein DnaI
  • replicon: chromosome
  • strand: -
  • coordinates: 1784903..1785823
  • length: 921
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGAAGCAATTTAAAAGTATAATTAACACGTCGCAGGACTTTGAAAAAAGAATAGAAAAG
    ATAAAAAAAGAAGTAATCAATGACCCAGATGTTAAGCAATTTTTGGAAGCGCATCGAGCT
    GAATTAACGAATGCTATGATTGATGAAGACTTAAATGTGTTACAAGAGTATAAAGATCAA
    CAAAAACATTATGACGGTCATAAATTTGCTGATTGTCCAAATTTCGTAAAGGGGCATGTG
    CCTGAGTTATATGTTGATAATAACCGAATTAAAATACGCTATTTACAATGCCCATGTAAA
    ATCAAGTACGACGAAGAACGCTTTGAAGCTGAGCTAATTACATCTCATCATATGCAACGA
    GATACTTTAAATGCCAAATTGAAAGATATTTATATGAATCATCGAGACCGTCTTGATGTA
    GCTATGGCAGCAGATGATATTTGTACAGCAATAACTAATGGGGAACAAGTGAAAGGCCTT
    TACCTTTATGGTCCATTTGGGACAGGTAAATCTTTTATTCTAGGTGCAATTGCGAATCAG
    CTCAAATCTAAGAAGGTACGTTCGACAATTATTTATTTACCGGAATTTATTAGAACATTA
    AAAGGTGGCTTTAAAGATGGTTCTTTTGAAAAGAAATTACATCGCGTAAGAGAAGCAAAC
    ATTTTAATGCTTGATGATATTGGGGCTGAAGAAGTGACTCCATGGGTGAGAGATGAGGTA
    ATTGGACCTTTGCTACATTATCGAATGGTTCATGAATTACCAACATTCTTTAGTTCTAAT
    TTTGACTATAGTGAATTGGAACATCATTTAGCGATGACTCGTGATGGTGAAGAGAAGACT
    AAAGCAGCACGTATTATTGAACGTGTCAAATCTTTGTCAACACCATACTTTTTATCAGGA
    GAAAATTTCAGAAACAATTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    921

⊟Protein[edit | edit source]

Protein Data Bank: 5HE8
Protein Data Bank: 5HE9

⊟General[edit | edit source]

  • locus tag: SAUSA300_RS08895 [old locus tag: SAUSA300_1630 ]
  • symbol: SAUSA300_RS08895
  • description: primosomal protein DnaI
  • length: 306
  • theoretical pI: 7.15118
  • theoretical MW: 35635.5
  • GRAVY: -0.589543

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing DNA metabolism DNA replication, recombination, and repair chromosomal replication initiator protein DnaA (TIGR00362; HMM-score: 38.3)
    and 6 more
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DnaA regulatory inactivator Hda (TIGR03420; HMM-score: 21)
    AAA family ATPase, CDC48 subfamily (TIGR01243; HMM-score: 17.9)
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides proteasome ATPase (TIGR03689; EC 3.6.4.8; HMM-score: 17.3)
    26S proteasome subunit P45 family (TIGR01242; HMM-score: 15.5)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair orc1/cdc6 family replication initiation protein (TIGR02928; HMM-score: 13.7)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair Holliday junction DNA helicase RuvB (TIGR00635; EC 3.6.4.12; HMM-score: 11.5)
  • TheSEED: see SAUSA300_1630
  • PFAM:
    no clan defined DnaI_N; Primosomal protein DnaI N-terminus (PF07319; HMM-score: 94.4)
    and 16 more
    P-loop_NTPase (CL0023) IstB_IS21; IstB-like ATP binding protein (PF01695; HMM-score: 36.8)
    Bac_DnaA; Bacterial dnaA protein (PF00308; HMM-score: 31.3)
    AAA; ATPase family associated with various cellular activities (AAA) (PF00004; HMM-score: 24.5)
    AFG1_ATPase; AFG1-like ATPase (PF03969; HMM-score: 21.6)
    PIF1; PIF1-like helicase (PF05970; HMM-score: 18.6)
    AAA_16; AAA ATPase domain (PF13191; HMM-score: 17)
    PhoH; PhoH-like protein (PF02562; HMM-score: 16.2)
    TsaE; Threonylcarbamoyl adenosine biosynthesis protein TsaE (PF02367; HMM-score: 14.3)
    RNA_helicase; RNA helicase (PF00910; HMM-score: 13.8)
    KAP_NTPase; KAP family P-loop domain (PF07693; HMM-score: 13.3)
    AAA_22; AAA domain (PF13401; HMM-score: 13.2)
    AAA_5; AAA domain (dynein-related subfamily) (PF07728; HMM-score: 12.3)
    ATPase_2; ATPase domain predominantly from Archaea (PF01637; HMM-score: 12.2)
    AAA_30; AAA domain (PF13604; HMM-score: 12.1)
    DUF815; Protein of unknown function (DUF815) (PF05673; HMM-score: 11.9)
    T3SS-hook (CL0424) Flg_hook; Flagellar hook-length control protein FliK (PF02120; HMM-score: 11.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003359
    • TAT(Tat/SPI): 0.000484
    • LIPO(Sec/SPII): 0.000445
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKQFKSIINTSQDFEKRIEKIKKEVINDPDVKQFLEAHRAELTNAMIDEDLNVLQEYKDQQKHYDGHKFADCPNFVKGHVPELYVDNNRIKIRYLQCPCKIKYDEERFEAELITSHHMQRDTLNAKLKDIYMNHRDRLDVAMAADDICTAITNGEQVKGLYLYGPFGTGKSFILGAIANQLKSKKVRSTIIYLPEFIRTLKGGFKDGSFEKKLHRVREANILMLDDIGAEEVTPWVRDEVIGPLLHYRMVHELPTFFSSNFDYSELEHHLAMTRDGEEKTKAARIIERVKSLSTPYFLSGENFRNN

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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