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PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

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⊟Summary[edit | edit source]

Contents

  • 1 Summary
  • 2 Genome View
  • 3 Gene
    • 3.1 General
    • 3.2 Accession numbers
    • 3.3 Phenotype
    • 3.4 DNA sequence
  • 4 Protein
    • 4.1 General
    • 4.2 Function
    • 4.3 Structure, modifications & cofactors
    • 4.4 Localization
    • 4.5 Accession numbers
    • 4.6 Protein sequence
    • 4.7 Experimental data
  • 5 Expression & Regulation
    • 5.1 Operon
    • 5.2 Regulation
    • 5.3 Transcription pattern
    • 5.4 Protein synthesis (provided by Aureolib)
    • 5.5 Protein stability
  • 6 Biological Material
    • 6.1 Mutants
    • 6.2 Expression vector
    • 6.3 lacZ fusion
    • 6.4 GFP fusion
    • 6.5 two-hybrid system
    • 6.6 FLAG-tag construct
    • 6.7 Antibody
  • 7 Other Information
  • 8 Literature
    • 8.1 References
    • 8.2 Relevant publications
  • organism: Staphylococcus aureus N315
  • locus tag: SA1448 [new locus tag: SA_RS08160 ]
  • pan locus tag?: SAUPAN004218000
  • symbol: SA1448
  • pan gene symbol?: —
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA1448 [new locus tag: SA_RS08160 ]
  • symbol: SA1448
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1651966..1652634
  • length: 669
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

  • Gene ID: 1124290 NCBI
  • RefSeq: NP_374733 NCBI
  • BioCyc: see SA_RS08160
  • MicrobesOnline: 103759 MicrobesOnline

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGATAGATCAACAAACAATTTATCAATACATACAAAATGGAAAAATAGAAGAAGCGTTA
    CAAGCATTGTTCGGAAATATCGAAGAAGATCCTACAATTATTGAAAATTATATTAATGCT
    GGTATCGTACTTGCTGATGCGAATGAGATTGAAAAGGCAGAGCGTTTTTTCCAAAAAGCT
    TTAACAATAGATCCGAAGAATGGCGTCGTATTTTATAATCTAGCAAATGTATATTATAAT
    CAGCAACGTTATCAAGAAGCTATTAAATTATATCAACAAGCATTACAAACAGAGATTGAA
    CAAGTTGATTGTAATTATATGATCGGTATGGCGTTTAATCAGTTAGAATCATTTAAGCTG
    GCATTGCCGTATTTAATGACTGCTGCGGAACTAGATAAAGACAAAGATGCAGAAGTTCAA
    TTTCAATATGGTCTTGTATTATGTCAATTAGAAATGTTTAATGAAGCCATAACTCAACTT
    AAACATGTATTAACGATTGATAAAAATCATGTTGATGCAAGATACAATTTGGGCTTAGCG
    TTATTTATGAAAAATGAAGATATTGATGAAGCAATAACTCATTTTAAAGAAGCTGTGACT
    ATCGACCCTAAACACTTATTAAGTCAGCATGCGCTGAAAACATTCACTAAAATGAAAGAG
    GAGGAGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    669

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA1448 [new locus tag: SA_RS08160 ]
  • symbol: SA1448
  • description: hypothetical protein
  • length: 222
  • theoretical pI: 4.37482
  • theoretical MW: 25718.1
  • GRAVY: -0.295946

⊟Function[edit | edit source]

  • ⊞TIGRFAM:
    type IV pilus biogenesis/stability protein PilW (TIGR02521; HMM-score: 73.5)
    putative PEP-CTERM system TPR-repeat lipoprotein (TIGR02917; HMM-score: 69)
    type III secretion low calcium response chaperone LcrH/SycD (TIGR02552; HMM-score: 59.2)
    and 7 more
    Metabolism Transport and binding proteins Amino acids, peptides and amines mitochondrial precursor proteins import receptor (TIGR00990; HMM-score: 53.8)
    tol-pal system protein YbgF (TIGR02795; HMM-score: 32.7)
    putative peptide modification system cyclase (TIGR04510; HMM-score: 25.8)
    Unknown function General heme biosynthesis-associated TPR protein (TIGR00540; HMM-score: 24.5)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Salvage of nucleosides and nucleotides adenine phosphoribosyltransferase (TIGR01090; EC 2.4.2.7; HMM-score: 23.5)
    pentatricopeptide repeat domain (TIGR00756; HMM-score: 22.7)
    poly-beta-1,6 N-acetyl-D-glucosamine export porin PgaA (TIGR03939; HMM-score: 20.4)
  • TheSEED  :
    • TPR repeat-containing protein YrrB
  • ⊞PFAM:
    TPR (CL0020) TPR_1; Tetratricopeptide repeat (PF00515; HMM-score: 99.5)
    TPR_2; Tetratricopeptide repeat (PF07719; HMM-score: 97.6)
    TPR_8; Tetratricopeptide repeat (PF13181; HMM-score: 93.1)
    TPR_11; TPR repeat (PF13414; HMM-score: 87.5)
    TPR_12; Tetratricopeptide repeat (PF13424; HMM-score: 79.7)
    and 42 more
    TPR_19; Tetratricopeptide repeat (PF14559; HMM-score: 68.6)
    TPR_17; Tetratricopeptide repeat (PF13431; HMM-score: 66.5)
    TPR_14; Tetratricopeptide repeat (PF13428; HMM-score: 62.6)
    TPR_16; Tetratricopeptide repeat (PF13432; HMM-score: 60.4)
    ANAPC3; Anaphase-promoting complex, cyclosome, subunit 3 (PF12895; HMM-score: 55.4)
    TPR_10; Tetratricopeptide repeat (PF13374; HMM-score: 51.2)
    TPR_7; Tetratricopeptide repeat (PF13176; HMM-score: 47.8)
    TPR_CcmH_CycH; Cytochrome c-type biogenesis protein H TPR domain (PF23914; HMM-score: 44.7)
    TPR_6; Tetratricopeptide repeat (PF13174; HMM-score: 43.3)
    TPR_20; Tetratricopeptide repeat (PF14561; HMM-score: 35.1)
    TPR_Slam; Surface lipoprotein assembly modifier, N-terminal TPR repeats (PF24575; HMM-score: 35)
    T7SS_EccA1_N; T7SS, ESX-1 secretion system protein EccA1, N-terminal domain (PF21545; HMM-score: 32.9)
    TPR_9; Tetratricopeptide repeat (PF13371; HMM-score: 30.4)
    TPR-S; Tetratricopeptide Repeats-Sensor (PF20308; HMM-score: 29.3)
    TOM20_plant; Plant specific mitochondrial import receptor subunit TOM20 (PF06552; HMM-score: 28.6)
    PPR; PPR repeat (PF01535; HMM-score: 25.5)
    ARM_TT21_C; Tetratricopeptide repeat protein 21 C-terminal ARM domain (PF25063; HMM-score: 24.9)
    TPR_NPHP3; Nephrocystin-3 TPR domain (PF24885; HMM-score: 23.2)
    TPR_15; Tetratricopeptide repeat (PF13429; HMM-score: 22)
    ARM_TT21_4th; Tetratricopeptide repeat protein 21 forth ARM domain (PF25068; HMM-score: 21.4)
    ARM_TT21_N; Tetratricopeptide repeat protein 21 N-terminal ARM repeat (PF25062; HMM-score: 20.3)
    TPR_3; Tetratricopeptide repeat (PF07720; HMM-score: 19.9)
    ARM_TT21_5th; TT21 fifth ARM repeats domain (PF25064; HMM-score: 19.7)
    ARM_TT21; Tetratricopeptide repeat protein 21 ARM repeat (PF25058; HMM-score: 19.4)
    E_motif; E motif (PF20431; HMM-score: 17.7)
    MIT (CL0745) MIT; MIT (microtubule interacting and transport) domain (PF04212; HMM-score: 17.4)
    TPR (CL0020) PPR_2; PPR repeat family (PF13041; HMM-score: 17.2)
    TPR_4; Tetratricopeptide repeat (PF07721; HMM-score: 16.8)
    NatA_aux_su; N-terminal acetyltransferase A, auxiliary subunit (PF12569; HMM-score: 16.5)
    RNPP_C; RNPP family C-terminal domain (PF18768; HMM-score: 16.2)
    no clan defined MatP; MatP N-terminal domain (PF06303; HMM-score: 15.8)
    TPR (CL0020) TPR_27; Plant tetratrico peptide repeats (PF23310; HMM-score: 14.3)
    Hect (CL0552) HECT_2; HECT-like Ubiquitin-conjugating enzyme (E2)-binding (PF09814; HMM-score: 14)
    TPR (CL0020) TPR_P4H; Prolyl 4-hydroxylase peptide-substrate-binding domain (PF23558; HMM-score: 13.5)
    no clan defined DUF1512; Protein of unknown function (DUF1512) N-terminal domain (PF07431; HMM-score: 13.4)
    TPR (CL0020) Rapsyn_N; Rapsyn N-terminal myristoylation and linker region (PF10579; HMM-score: 12.7)
    TPR_21; Tetratricopeptide repeat-like domain (PF09976; HMM-score: 12.4)
    TPR_MalT; MalT-like TPR region (PF17874; HMM-score: 12.4)
    EAD11; Effector-associated domain 11 (PF19964; HMM-score: 12.1)
    Fis1_TPR_C; Fis1 C-terminal tetratricopeptide repeat (PF14853; HMM-score: 11.5)
    Sel1; Sel1 repeat (PF08238; HMM-score: 10.9)
    Clathrin; Region in Clathrin and VPS (PF00637; HMM-score: 10.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.8874
    • Cytoplasmic Membrane Score: 0.066
    • Cell wall & surface Score: 0.0166
    • Extracellular Score: 0.0299
  • ⊞LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003148
    • TAT(Tat/SPI): 0.000166
    • LIPO(Sec/SPII): 0.000337
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI: 15927200 NCBI
  • RefSeq: NP_374733 NCBI
  • UniProt: A0A0H3JM81 UniProt

⊟Protein sequence[edit | edit source]

  • MIDQQTIYQYIQNGKIEEALQALFGNIEEDPTIIENYINAGIVLADANEIEKAERFFQKALTIDPKNGVVFYNLANVYYNQQRYQEAIKLYQQALQTEIEQVDCNYMIGMAFNQLESFKLALPYLMTAAELDKDKDAEVQFQYGLVLCQLEMFNEAITQLKHVLTIDKNHVDARYNLGLALFMKNEDIDEAITHFKEAVTIDPKHLLSQHALKTFTKMKEEE

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

  • MicrobesOnline: SA1447 < SA1448

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

  • S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

  • Aureolib: no data available

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=SA1448&oldid=89824"
  • This page was last edited on 11 March 2016, at 07:36.
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