PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA_RS08160 [old locus tag: SA1448 ]
• pan locus tag^?: SAUPAN004218000
• symbol: SA_RS08160
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA_RS08160 [old locus tag: SA1448 ]
• symbol: SA_RS08160
• product: hypothetical protein
• replicon: chromosome
• strand: -
• coordinates: 1651966..1652634
• length: 669
• essential: no DEG other strains

⊟Accession numbers

• Location: NC_002745 (1651966..1652634) NCBI
• BioCyc: SA_RS08160 BioCyc
• MicrobesOnline: see SA1448

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  661
  
  ATGATAGATCAACAAACAATTTATCAATACATACAAAATGGAAAAATAGAAGAAGCGTTA
  CAAGCATTGTTCGGAAATATCGAAGAAGATCCTACAATTATTGAAAATTATATTAATGCT
  GGTATCGTACTTGCTGATGCGAATGAGATTGAAAAGGCAGAGCGTTTTTTCCAAAAAGCT
  TTAACAATAGATCCGAAGAATGGCGTCGTATTTTATAATCTAGCAAATGTATATTATAAT
  CAGCAACGTTATCAAGAAGCTATTAAATTATATCAACAAGCATTACAAACAGAGATTGAA
  CAAGTTGATTGTAATTATATGATCGGTATGGCGTTTAATCAGTTAGAATCATTTAAGCTG
  GCATTGCCGTATTTAATGACTGCTGCGGAACTAGATAAAGACAAAGATGCAGAAGTTCAA
  TTTCAATATGGTCTTGTATTATGTCAATTAGAAATGTTTAATGAAGCCATAACTCAACTT
  AAACATGTATTAACGATTGATAAAAATCATGTTGATGCAAGATACAATTTGGGCTTAGCG
  TTATTTATGAAAAATGAAGATATTGATGAAGCAATAACTCATTTTAAAGAAGCTGTGACT
  ATCGACCCTAAACACTTATTAAGTCAGCATGCGCTGAAAACATTCACTAAAATGAAAGAG
  GAGGAGTAA
  60
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  660
  669
  

⊟Protein

⊟General

• locus tag: SA_RS08160 [old locus tag: SA1448 ]
• symbol: SA_RS08160
• description: hypothetical protein
• length: 222
• theoretical pI: 4.37482
• theoretical MW: 25718.1
• GRAVY: -0.295946

⊟Function

• TIGRFAM:
  type IV pilus biogenesis/stability protein PilW (TIGR02521; HMM-score: 73.5)
  putative PEP-CTERM system TPR-repeat lipoprotein (TIGR02917; HMM-score: 69)
  type III secretion low calcium response chaperone LcrH/SycD (TIGR02552; HMM-score: 59.2)
  and 7 more
  Metabolism Transport and binding proteins Amino acids, peptides and amines mitochondrial precursor proteins import receptor (TIGR00990; HMM-score: 53.8)
  tol-pal system protein YbgF (TIGR02795; HMM-score: 32.7)
  putative peptide modification system cyclase (TIGR04510; HMM-score: 25.8)
  Unknown function General heme biosynthesis-associated TPR protein (TIGR00540; HMM-score: 24.5)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Salvage of nucleosides and nucleotides adenine phosphoribosyltransferase (TIGR01090; EC 2.4.2.7; HMM-score: 23.5)
  pentatricopeptide repeat domain (TIGR00756; HMM-score: 22.7)
  poly-beta-1,6 N-acetyl-D-glucosamine export porin PgaA (TIGR03939; HMM-score: 20.4)
• TheSEED: see SA1448
• PFAM:
  TPR (CL0020) TPR_1; Tetratricopeptide repeat (PF00515; HMM-score: 99.5)
  TPR_2; Tetratricopeptide repeat (PF07719; HMM-score: 97.6)
  TPR_8; Tetratricopeptide repeat (PF13181; HMM-score: 93.1)
  TPR_11; TPR repeat (PF13414; HMM-score: 87.5)
  TPR_12; Tetratricopeptide repeat (PF13424; HMM-score: 79.7)
  and 42 more
  TPR_19; Tetratricopeptide repeat (PF14559; HMM-score: 68.6)
  TPR_17; Tetratricopeptide repeat (PF13431; HMM-score: 66.5)
  TPR_14; Tetratricopeptide repeat (PF13428; HMM-score: 62.6)
  TPR_16; Tetratricopeptide repeat (PF13432; HMM-score: 60.4)
  ANAPC3; Anaphase-promoting complex, cyclosome, subunit 3 (PF12895; HMM-score: 55.4)
  TPR_10; Tetratricopeptide repeat (PF13374; HMM-score: 51.2)
  TPR_7; Tetratricopeptide repeat (PF13176; HMM-score: 47.8)
  TPR_CcmH_CycH; Cytochrome c-type biogenesis protein H TPR domain (PF23914; HMM-score: 44.7)
  TPR_6; Tetratricopeptide repeat (PF13174; HMM-score: 43.3)
  TPR_20; Tetratricopeptide repeat (PF14561; HMM-score: 35.1)
  TPR_Slam; Surface lipoprotein assembly modifier, N-terminal TPR repeats (PF24575; HMM-score: 35)
  T7SS_EccA1_N; T7SS, ESX-1 secretion system protein EccA1, N-terminal domain (PF21545; HMM-score: 32.9)
  TPR_9; Tetratricopeptide repeat (PF13371; HMM-score: 30.4)
  TPR-S; Tetratricopeptide Repeats-Sensor (PF20308; HMM-score: 29.3)
  TOM20_plant; Plant specific mitochondrial import receptor subunit TOM20 (PF06552; HMM-score: 28.6)
  PPR; PPR repeat (PF01535; HMM-score: 25.5)
  ARM_TT21_C; Tetratricopeptide repeat protein 21 C-terminal ARM domain (PF25063; HMM-score: 24.9)
  TPR_NPHP3; Nephrocystin-3 TPR domain (PF24885; HMM-score: 23.2)
  TPR_15; Tetratricopeptide repeat (PF13429; HMM-score: 22)
  ARM_TT21_4th; Tetratricopeptide repeat protein 21 forth ARM domain (PF25068; HMM-score: 21.4)
  ARM_TT21_N; Tetratricopeptide repeat protein 21 N-terminal ARM repeat (PF25062; HMM-score: 20.3)
  TPR_3; Tetratricopeptide repeat (PF07720; HMM-score: 19.9)
  ARM_TT21_5th; TT21 fifth ARM repeats domain (PF25064; HMM-score: 19.7)
  ARM_TT21; Tetratricopeptide repeat protein 21 ARM repeat (PF25058; HMM-score: 19.4)
  E_motif; E motif (PF20431; HMM-score: 17.7)
  MIT (CL0745) MIT; MIT (microtubule interacting and transport) domain (PF04212; HMM-score: 17.4)
  TPR (CL0020) PPR_2; PPR repeat family (PF13041; HMM-score: 17.2)
  TPR_4; Tetratricopeptide repeat (PF07721; HMM-score: 16.8)
  NatA_aux_su; N-terminal acetyltransferase A, auxiliary subunit (PF12569; HMM-score: 16.5)
  RNPP_C; RNPP family C-terminal domain (PF18768; HMM-score: 16.2)
  no clan defined MatP; MatP N-terminal domain (PF06303; HMM-score: 15.8)
  TPR (CL0020) TPR_27; Plant tetratrico peptide repeats (PF23310; HMM-score: 14.3)
  Hect (CL0552) HECT_2; HECT-like Ubiquitin-conjugating enzyme (E2)-binding (PF09814; HMM-score: 14)
  TPR (CL0020) TPR_P4H; Prolyl 4-hydroxylase peptide-substrate-binding domain (PF23558; HMM-score: 13.5)
  no clan defined DUF1512; Protein of unknown function (DUF1512) N-terminal domain (PF07431; HMM-score: 13.4)
  TPR (CL0020) Rapsyn_N; Rapsyn N-terminal myristoylation and linker region (PF10579; HMM-score: 12.7)
  TPR_21; Tetratricopeptide repeat-like domain (PF09976; HMM-score: 12.4)
  TPR_MalT; MalT-like TPR region (PF17874; HMM-score: 12.4)
  EAD11; Effector-associated domain 11 (PF19964; HMM-score: 12.1)
  Fis1_TPR_C; Fis1 C-terminal tetratricopeptide repeat (PF14853; HMM-score: 11.5)
  Sel1; Sel1 repeat (PF08238; HMM-score: 10.9)
  Clathrin; Region in Clathrin and VPS (PF00637; HMM-score: 10.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.8874
  • Cytoplasmic Membrane Score: 0.066
  • Cell wall & surface Score: 0.0166
  • Extracellular Score: 0.0299
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.003148
  • TAT(Tat/SPI): 0.000166
  • LIPO(Sec/SPII): 0.000337
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446489163 NCBI
• RefSeq: WP_000567017 NCBI
• UniProt: see SA1448

⊟Protein sequence

• MIDQQTIYQYIQNGKIEEALQALFGNIEEDPTIIENYINAGIVLADANEIEKAERFFQKALTIDPKNGVVFYNLANVYYNQQRYQEAIKLYQQALQTEIEQVDCNYMIGMAFNQLESFKLALPYLMTAAELDKDKDAEVQFQYGLVLCQLEMFNEAITQLKHVLTIDKNHVDARYNLGLALFMKNEDIDEAITHFKEAVTIDPKHLLSQHALKTFTKMKEEE

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications