From AureoWiki
Jump to navigation Jump to search

NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01724
  • pan locus tag?: SAUPAN004218000
  • symbol: SAOUHSC_01724
  • pan gene symbol?: —
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_01724
  • symbol: SAOUHSC_01724
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1630711..1631379
  • length: 669
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGATAGATCAACAAACAATTTATCAATACATACAAAATGGAAAAATAGAAGAAGCGTTA
    CAAGCATTGTTCGGAAATATCGAAGAAAATCCTACAATTATTGAAAATTATATTAATGCT
    GGTATCGTACTTGCTGATGCGAATGAGATTGAAAAGGCAGAGCGTTTTTTCCAAAAAGCT
    TTAACAATAGATCCGAAGAATGGCGTCGTATTTTTTAATCTAGCAAATGTATATTATAAT
    CAGCAACGTTATCAAGAAGCTATTAAATTATATCAACAAGCATTACAAACAGAGATTGAA
    CAAGTTGATTGTAATTATATGATCGGTATGGCGTTTAATCAGTTAGAATCATTTAAGCTG
    GCATTGCCGTATTTAATGACTGCTGCGGAACTAGATAAAGACAAAGATGCAGAAGTTCAA
    TTTCAATATGGTCTTGTATTATGTCAATTAGAAATGTTTAATGAAGCCATAACTCAACTT
    AAACATGTATTAACGATTGATAAAAATCATGTTGATGCAAGATACAATTTGGGCTTAGCG
    TTATTTATGAAAAATGAAGATATTGATGAAGCAATAACTCATTTTAAAGAAGCTGTGACT
    ATCGACCCTAAACACTTATTAAGTCAGCATGCGCTGAAAACATTCACTAAAATGAAAGAG
    GAGGAGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    669

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_01724
  • symbol: SAOUHSC_01724
  • description: hypothetical protein
  • length: 222
  • theoretical pI: 4.41965
  • theoretical MW: 25701.1
  • GRAVY: -0.277477

⊟Function[edit | edit source]

  • TIGRFAM:
    type IV pilus biogenesis/stability protein PilW (TIGR02521; HMM-score: 72.1)
    putative PEP-CTERM system TPR-repeat lipoprotein (TIGR02917; HMM-score: 70.8)
    and 8 more
    type III secretion low calcium response chaperone LcrH/SycD (TIGR02552; HMM-score: 56.5)
    Metabolism Transport and binding proteins Amino acids, peptides and amines mitochondrial precursor proteins import receptor (TIGR00990; HMM-score: 49.8)
    tol-pal system protein YbgF (TIGR02795; HMM-score: 31.4)
    putative peptide modification system cyclase (TIGR04510; HMM-score: 26.2)
    Unknown function General heme biosynthesis-associated TPR protein (TIGR00540; HMM-score: 24.5)
    pentatricopeptide repeat domain (TIGR00756; HMM-score: 21.9)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Salvage of nucleosides and nucleotides adenine phosphoribosyltransferase (TIGR01090; EC 2.4.2.7; HMM-score: 21.4)
    poly-beta-1,6 N-acetyl-D-glucosamine export porin PgaA (TIGR03939; HMM-score: 20.7)
  • TheSEED  :
    • Tetratricopeptide repeat (TPR) family protein
  • PFAM:
    TPR (CL0020) TPR_1; Tetratricopeptide repeat (PF00515; HMM-score: 100)
    TPR_2; Tetratricopeptide repeat (PF07719; HMM-score: 99.7)
    TPR_8; Tetratricopeptide repeat (PF13181; HMM-score: 93.7)
    TPR_11; TPR repeat (PF13414; HMM-score: 86.9)
    TPR_12; Tetratricopeptide repeat (PF13424; HMM-score: 80.2)
    and 23 more
    TPR_17; Tetratricopeptide repeat (PF13431; HMM-score: 64.5)
    TPR_19; Tetratricopeptide repeat (PF14559; HMM-score: 63.2)
    TPR_16; Tetratricopeptide repeat (PF13432; HMM-score: 59.9)
    TPR_14; Tetratricopeptide repeat (PF13428; HMM-score: 59.4)
    ANAPC3; Anaphase-promoting complex, cyclosome, subunit 3 (PF12895; HMM-score: 53.3)
    TPR_7; Tetratricopeptide repeat (PF13176; HMM-score: 52.4)
    TPR_10; Tetratricopeptide repeat (PF13374; HMM-score: 47.6)
    TPR_6; Tetratricopeptide repeat (PF13174; HMM-score: 42.5)
    TPR_20; Tetratricopeptide repeat (PF14561; HMM-score: 35.5)
    TOM20_plant; Plant specific mitochondrial import receptor subunit TOM20 (PF06552; HMM-score: 28.3)
    TPR_9; Tetratricopeptide repeat (PF13371; HMM-score: 28.1)
    TPR_3; Tetratricopeptide repeat (PF07720; HMM-score: 26.8)
    PPR; PPR repeat (PF01535; HMM-score: 24.8)
    TPR_15; Tetratricopeptide repeat (PF13429; HMM-score: 22.2)
    no clan defined MIT; MIT (microtubule interacting and transport) domain (PF04212; HMM-score: 21.5)
    TPR (CL0020) PPR_2; PPR repeat family (PF13041; HMM-score: 18)
    NARP1; NMDA receptor-regulated protein 1 (PF12569; HMM-score: 16.8)
    TPR_21; Tetratricopeptide repeat-like domain (PF09976; HMM-score: 16.4)
    TPR_4; Tetratricopeptide repeat (PF07721; HMM-score: 15.8)
    Hect (CL0552) HECT_2; HECT-like Ubiquitin-conjugating enzyme (E2)-binding (PF09814; HMM-score: 15.3)
    TPR (CL0020) Fis1_TPR_C; Fis1 C-terminal tetratricopeptide repeat (PF14853; HMM-score: 13)
    Clathrin; Region in Clathrin and VPS (PF00637; HMM-score: 12.1)
    Sel1; Sel1 repeat (PF08238; HMM-score: 11.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004087
    • TAT(Tat/SPI): 0.000163
    • LIPO(Sec/SPII): 0.000383
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MIDQQTIYQYIQNGKIEEALQALFGNIEENPTIIENYINAGIVLADANEIEKAERFFQKALTIDPKNGVVFFNLANVYYNQQRYQEAIKLYQQALQTEIEQVDCNYMIGMAFNQLESFKLALPYLMTAAELDKDKDAEVQFQYGLVLCQLEMFNEAITQLKHVLTIDKNHVDARYNLGLALFMKNEDIDEAITHFKEAVTIDPKHLLSQHALKTFTKMKEEE

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ 3.0 3.1 3.2 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=SAOUHSC_01724&oldid=100019"