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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 03-AUG-2016

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⊟Summary[edit | edit source]

Contents

  • 1 Summary
  • 2 Genome View
  • 3 Gene
    • 3.1 General
    • 3.2 Accession numbers
    • 3.3 Phenotype
    • 3.4 DNA sequence
  • 4 Protein
    • 4.1 General
    • 4.2 Function
    • 4.3 Structure, modifications & cofactors
    • 4.4 Localization
    • 4.5 Accession numbers
    • 4.6 Protein sequence
    • 4.7 Experimental data
  • 5 Expression & Regulation
    • 5.1 Operon
    • 5.2 Regulation
    • 5.3 Transcription pattern
    • 5.4 Protein synthesis (provided by Aureolib)
    • 5.5 Protein stability
  • 6 Biological Material
    • 6.1 Mutants
    • 6.2 Expression vector
    • 6.3 lacZ fusion
    • 6.4 GFP fusion
    • 6.5 two-hybrid system
    • 6.6 FLAG-tag construct
    • 6.7 Antibody
  • 7 Other Information
  • 8 Literature
    • 8.1 References
    • 8.2 Relevant publications
  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01779
  • pan locus tag?: SAUPAN004291000
  • symbol: tig
  • pan gene symbol?: tig
  • synonym:
  • product: trigger factor

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_01779
  • symbol: tig
  • product: trigger factor
  • replicon: chromosome
  • strand: -
  • coordinates: 1679287..1680588
  • length: 1302
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

  • Gene ID: 3919697 NCBI
  • RefSeq: YP_500284 NCBI
  • BioCyc: G1I0R-1653 BioCyc
  • MicrobesOnline: 1290198 MicrobesOnline

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGACAGCAACTTGGGAAAAAAAGGAAGGTAACGAAGGTTTATTAACTGTTACTGTTCCT
    GCAGAAAAAGTAAACAAAGCGTTAGATCAAGCATTCAAAAAAGTGGTTAAACAAATTAAC
    GTACCTGGATTCCGTAAAGGTAAAGTGCCACGTCCAATTTTTGAACAACGCTTTGGTGTA
    GAAGCATTATATCAAGATGCTATCGACATTTTATTACCAGATGCTTATGGTGAAGCAATT
    GACGAAACTGATATTAAACCAGTTGCACAACCAGAAGTAAGTGTTACTCAAATTGAAAAA
    GGTAAAGATTTCATTTTTGAAGCAACAGTTACAGTTGAGCCAGAAGTTAAATTAGGAGAC
    TATAAAGGTCTTGAAATTGAAAAACAAGAAACTGAATTATCTGATGATGAGTTACAAGAA
    GCGATTGACCACAGCTTAGGACATTTAGCTGAAATGGTCGTTAAAGAAGATGGTGTTGTT
    GAAAATGGCGACACAGTTAACATCGACTTTAGTGGTTCAGTTGACGGAGAAGAATTCGAA
    GGTGGACAAGCTGAAGGTTACGATTTAGAAATCGGTTCAGGTTCATTCATACCTGGTTTC
    GAAGAGCAATTAGAAGGTATGAAAGTTGACGAAGAAAAAGATGTTGTCGTAACATTCCCA
    GAAGAATACCATGCTGAAGAATTAGCCGGTAAAGAAGCAACTTTCAAAACAAAAGTTAAC
    GAAATTAAATTTAAAGAAGTCCCAGAATTAACAGATGAAATTGCTAATGAATTAGATGCA
    GAAGCAAATACAGTAGACGAGTACAAAGAAAACTTACGTAAACGTTTAGCTGAACAAAAA
    GCTACAGATGCTGAAAATGTTGAAAAAGAAGAAGCGATTACAAAAGCTACTGATAATACA
    ACAATCGATATTCCTGAAGCAATGGTTAATACTGAATTAGATCGTATGGTGTCTGAATTT
    GCACAAAGAATTCAACAACAAGGTTTAGATTTACAAACGTACTTCCAAATCTCAGGTCAA
    GATGAAACTCAATTAAGAGAGCAAATGAAAGACGATGCAGAACAACGTGTTAAAACTAAC
    TTAACTTTAACTGCGATCGCTGAAGCTGAAAAAATCGAAGCTACTGATGAAGATATCGAT
    AAAGAATTAGAAAAAATGAGTAAACAATTTAATATCTCAGTTGAAGATATCAAAAATACT
    TTAGGTAATACTGATATCATTAAAAATGATGTTCGTATCCAAAAAGTTATCGATTTATTA
    AGAGATAACGCAAAGTTCGTTGAAGGAACTAAAGAAGATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1302

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_01779
  • symbol: Tig
  • description: trigger factor
  • length: 433
  • theoretical pI: 4.05625
  • theoretical MW: 48608.6
  • GRAVY: -0.642956

⊟Function[edit | edit source]

  • ⊞reaction:
    EC 5.2.1.8?  ExPASy
    Peptidylprolyl isomerase Peptidylproline (omega=180) = peptidylproline (omega=0)
  • ⊞TIGRFAM:
    Genetic information processing Protein fate Protein folding and stabilization trigger factor (TIGR00115; EC 5.2.1.8; HMM-score: 451.8)
    and 1 more
    Metabolism Fatty acid and phospholipid metabolism Biosynthesis poly(hydroxyalkanoate) granule-associated protein (TIGR01837; HMM-score: 8.2)
  • ⊞TheSEED  :
    • Trigger factor (EC 5.2.1.8)
    Bacterial Cell Division  Cell division trigger factor (EC 5.2.1.8)
    and 1 more
    RP Bacterial Cell Division  Cell division trigger factor (EC 5.2.1.8)
  • ⊞PFAM:
    no clan defined Trigger_N; Bacterial trigger factor protein (TF) (PF05697; HMM-score: 172.6)
    Trigger_C (CL0262) Trigger_C; Bacterial trigger factor protein (TF) C-terminus (PF05698; HMM-score: 159.4)
    and 7 more
    FKBP (CL0487) FKBP_C; FKBP-type peptidyl-prolyl cis-trans isomerase (PF00254; HMM-score: 69.4)
    EF_hand (CL0220) EF-hand_14; EF-hand domain (PF17959; HMM-score: 13.6)
    HTH (CL0123) GerE; Bacterial regulatory proteins, luxR family (PF00196; HMM-score: 12.7)
    E-set (CL0159) DUF7350; Domain of unknown function (DUF7350) (PF24041; HMM-score: 12.6)
    TIM_barrel (CL0036) GH123_cat; Glycoside hydrolase 123, catalytic domain (PF13320; HMM-score: 9)
    XRCC4_N (CL0777) XRCC4; XRCC4 N-terminal domain (PF06632; HMM-score: 8.6)
    Trigger_C (CL0262) SurA_N_3; SurA-like N-terminal domain (PF13624; HMM-score: 8.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9993
    • Cytoplasmic Membrane Score: 0.0001
    • Cell wall & surface Score: 0.0001
    • Extracellular Score: 0.0005
  • ⊞LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003665
    • TAT(Tat/SPI): 0.000647
    • LIPO(Sec/SPII): 0.001578
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI: 88195480 NCBI
  • RefSeq: YP_500284 NCBI
  • UniProt: Q2FXQ6 UniProt
  • STRING: 93061.SAOUHSC_01779 STRING

⊟Protein sequence[edit | edit source]

  • MTATWEKKEGNEGLLTVTVPAEKVNKALDQAFKKVVKQINVPGFRKGKVPRPIFEQRFGVEALYQDAIDILLPDAYGEAIDETDIKPVAQPEVSVTQIEKGKDFIFEATVTVEPEVKLGDYKGLEIEKQETELSDDELQEAIDHSLGHLAEMVVKEDGVVENGDTVNIDFSGSVDGEEFEGGQAEGYDLEIGSGSFIPGFEEQLEGMKVDEEKDVVVTFPEEYHAEELAGKEATFKTKVNEIKFKEVPELTDEIANELDAEANTVDEYKENLRKRLAEQKATDAENVEKEEAITKATDNTTIDIPEAMVNTELDRMVSEFAQRIQQQGLDLQTYFQISGQDETQLREQMKDDAEQRVKTNLTLTAIAEAEKIEATDEDIDKELEKMSKQFNISVEDIKNTLGNTDIIKNDVRIQKVIDLLRDNAKFVEGTKED

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • ⊟interaction partners:
    SAOUHSC_00519(rplA)50S ribosomal protein L1  [3] (data from MRSA252)
    SAOUHSC_02509(rplB)50S ribosomal protein L2  [3] (data from MRSA252)
    SAOUHSC_02512(rplC)50S ribosomal protein L3  [3] (data from MRSA252)
    SAOUHSC_02511(rplD)50S ribosomal protein L4  [3] (data from MRSA252)
    SAOUHSC_02500(rplE)50S ribosomal protein L5  [3] (data from MRSA252)
    SAOUHSC_00520(rplJ)50S ribosomal protein L10  [3] (data from MRSA252)
    SAOUHSC_02492(rplO)50S ribosomal protein L15  [3] (data from MRSA252)
    SAOUHSC_02505(rplP)50S ribosomal protein L16  [3] (data from MRSA252)
    SAOUHSC_01211(rplS)50S ribosomal protein L19  [3] (data from MRSA252)
    SAOUHSC_01757(rplU)50S ribosomal protein L21  [3] (data from MRSA252)
    SAOUHSC_02507(rplV)50S ribosomal protein L22  [3] (data from MRSA252)
    SAOUHSC_02510(rplW)50S ribosomal protein L23  [3] (data from MRSA252)
    SAOUHSC_01232(rpsB)30S ribosomal protein S2  [3] (data from MRSA252)
    SAOUHSC_02506(rpsC)30S ribosomal protein S3  [3] (data from MRSA252)
    SAOUHSC_02494(rpsE)30S ribosomal protein S5  [3] (data from MRSA252)
    SAOUHSC_00348(rpsF)30S ribosomal protein S6  [3] (data from MRSA252)
    SAOUHSC_02487(rpsM)30S ribosomal protein S13  [3] (data from MRSA252)
    SAOUHSC_02503(rpsQ)30S ribosomal protein S17  [3] (data from MRSA252)
    SAOUHSC_00530elongation factor Tu  [3] (data from MRSA252)
    SAOUHSC_01040pyruvate dehydrogenase complex, E1 component subunit alpha  [3] (data from MRSA252)
    SAOUHSC_01041pyruvate dehydrogenase complex, E1 component subunit beta  [3] (data from MRSA252)
    SAOUHSC_01043dihydrolipoamide dehydrogenase  [3] (data from MRSA252)
    SAOUHSC_01150cell division protein FtsZ  [3] (data from MRSA252)
    SAOUHSC_01154hypothetical protein  [3] (data from MRSA252)
    SAOUHSC_01490DNA-binding protein HU  [3] (data from MRSA252)
    SAOUHSC_01806pyruvate kinase  [3] (data from MRSA252)
    SAOUHSC_01857hypothetical protein  [3] (data from MRSA252)
    SAOUHSC_0248630S ribosomal protein S11  [3] (data from MRSA252)
    SAOUHSC_02579hypothetical protein  [3] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

  • MicrobesOnline: clpX < tig
    predicted SigA promoter [4] : clpX < S703 < tig < SAOUHSC_01780 < SAOUHSC_01781 < SAOUHSC_01782
    predicted SigA promoter [4] : clpX < S703 < tig < SAOUHSC_01780 < SAOUHSC_01781 < SAOUHSC_01782 < rplT < rpmI < infC < S704 < S705

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

  • S.aureus Expression Data Browser:  [4] 
    Expression Data Browser
    ⊟Multi-gene expression profiles

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

  • Aureolib: data available for COL

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ Jump up to: 3.00 3.01 3.02 3.03 3.04 3.05 3.06 3.07 3.08 3.09 3.10 3.11 3.12 3.13 3.14 3.15 3.16 3.17 3.18 3.19 3.20 3.21 3.22 3.23 3.24 3.25 3.26 3.27 3.28 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)
  4. ↑ Jump up to: 4.0 4.1 4.2 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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  • This page was last edited on 11 March 2016, at 06:57.
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