PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA_RS10060 [old locus tag: SA1751 ]
• pan locus tag^?: SAUPAN005009000
• symbol: SA_RS10060
• pan gene symbol^?: eap/map
• synonym:
• product: protein map

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA_RS10060 [old locus tag: SA1751 ]
• symbol: SA_RS10060
• product: protein map
• replicon: chromosome
• strand: -
• coordinates: 2003333..2004763
• length: 1431
• essential: no DEG other strains

⊟Accession numbers

• Location: NC_002745 (2003333..2004763) NCBI
• BioCyc: SA_RS10060 BioCyc
• MicrobesOnline: see SA1751

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  1321
  1381
  
  ATGAAATTTAAGTCATTGATTACAACAACATTAGCATTAGGCGTTATAGCATCAACAGGA
  GCAAACTTTAATACTAACGAAGCATCTGCCGCAGCTAAGCCATTAGATAAATCATCAAGT
  ACATTACACCATGGACATTCTAACATCCAGATTCCATATACAATTACTGTGAACGGTACA
  AGCCAAAACATTTTATCAAGCTTAACATTTAATAAGAATCAAAATATTAGTTATAAAGAT
  ATAGAGAATAAAGTTAAATCAGTTTTATACTTTAATAGAGGTATTAGTGATATCGATTTA
  AGACTTTCAAAGCAAGCGGAATATACGGTTCATTTTAAAAATGGAACAAAAAGAGTTATC
  GATTTGAAATCAGGTATCTACACAGCTGACTTAATCAATACAAGTGACATTAAAGCTATC
  AGTGTTAACGTAGATACTAAAAAGCAACCTAAAGATAAAGCTAAAGCAAATGTTCAAGTG
  CCATATACAATCACAGTGAACGGCACAAGCCAAAACATTTTATCAAACCTAACATTTAAT
  AAAAATCAAAATATTAGTTACAAAGATTTAGAGGGTAAAGTTAAATCAGTTTTAGAATCA
  AATAGAGGTATTACTGATGTTGATTTAAGACTTTCGAAGCAAGCGAAATATACAGTTAAT
  TTTAAAAATGGAACGAAGAAAGTTATCGATTTGAAATCAGGTATTTACACAGCGAATTTA
  ATCAATTCAAGTGATATTAAAAGTATCAATATTAACGTAGATACAAAAAAACATATCGAA
  AATAAAGCTAAAAGAAACTATCAAGTTCCATATTCAATTAATCTAAATGGTACATCTACA
  AACATTTTATCGAATCTTTCATTTTCAAATAAACCTTGGACAAATTACAAAAATTTAACT
  AGTCAAATAAAATCAGTACTGAAGCATGATAGAGGTATTAGTGAACAAGATTTAAAATAT
  GCTAAGAAAGCTTATTATACTGTTTATTTTAAAAATGGTGGTAAAAGAATCTTACAGTTA
  AATTCAAAAAATTACACAGCAAACTTAGTTCATGCGAAAGATGTTAAGAGAATTGAAATT
  ACTGTTAAAACAGGAACTAAAGCGAAAGCAGACAGATATGTACCATACACAATTGCAGTA
  AATGGCACATCAACACCAATTTTATCAAAACTAAAAATTTCGAATAAACAATTAATTAGT
  TACAAATATTTAAACGACAAAGTGAAATCTGTATTAAAAAGTGAAAGAGGTATCAGTGAT
  CTTGACTTAAAATTTGCGAAACAAGCAAAATATACAGTATATTTCAAAAATGGAAAGAAA
  CAAGTAGTGAATTTAAAATCAGACATCTTTACACCTAATTTATTTAGTGCCAAAGATATT
  AAAAAGATTGATATTGATGTAAAACAATACACTAAATCAAAAAAAAAATAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1080
  1140
  1200
  1260
  1320
  1380
  1431
  

⊟Protein

⊟General

• locus tag: SA_RS10060 [old locus tag: SA1751 ]
• symbol: SA_RS10060
• description: protein map
• length: 476
• theoretical pI: 10.5909
• theoretical MW: 53376.8
• GRAVY: -0.552731

⊟Function

• TIGRFAM:
  modification target Cys-rich peptide, AC3_0185 family (TIGR04118; HMM-score: 12.5)
• TheSEED: see SA1751
• PFAM:
  no clan defined MAP; MAP domain (PF03642; HMM-score: 489.1)
  and 16 more
  SH3 (CL0010) DUF3553; Protein of unknown function (DUF3553) (PF12073; HMM-score: 24.4)
  DUF7141; Domain of unknown function (DUF7141) (PF23614; HMM-score: 22.5)
  no clan defined APS-reductase_C; Adenosine-5'-phosphosulfate reductase beta subunit (PF12139; HMM-score: 21.8)
  PLP_aminotran (CL0061) ComK; ComK protein (PF06338; HMM-score: 20.2)
  Homing_endonuc (CL0324) Hom_end; Homing endonuclease (PF05204; HMM-score: 18.8)
  E-set (CL0159) DUF5780; Family of unknown function (DUF5780) (PF19092; HMM-score: 17.3)
  SH3 (CL0010) Tudor_3; DNA repair protein Crb2 Tudor domain (PF18115; HMM-score: 16.8)
  Cache (CL0165) GAPES3; Gammaproteobacterial periplasmic sensor domain (PF17154; HMM-score: 15.5)
  no clan defined M_C; Protein M, C-terminal (PF22806; HMM-score: 13.7)
  Trefoil (CL0066) DUF569; Beta-trefoil domain of unknown function (DUF569) (PF04601; HMM-score: 11.7)
  E-set (CL0159) aRib; Atypical Rib domain (PF18938; HMM-score: 10.8)
  HMA (CL0704) HMA; Heavy-metal-associated domain (PF00403; HMM-score: 10.6)
  no clan defined Frataxin_Cyay; Frataxin-like domain (PF01491; HMM-score: 10.3)
  DUF7212; Family of unknown function (DUF7212) (PF23848; HMM-score: 7.8)
  Neisseria_TspB; Neisseria meningitidis TspB protein (PF05616; HMM-score: 7.7)
  Tcp10_C; T-complex protein 10 C-terminus (PF07202; HMM-score: 7.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 0
• DeepLocPro: Extracellular
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0.0001
  • Cell wall & surface Score: 0.3797
  • Extracellular Score: 0.6202
• LocateP:
• SignalP: Signal peptide SP(Sec/SPI) length 30 aa
  • SP(Sec/SPI): 0.89345
  • TAT(Tat/SPI): 0.004555
  • LIPO(Sec/SPII): 0.096731
  • Cleavage Site: CS pos: 30-31. ASA-AA. Pr: 0.5840
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 486219380 NCBI
• RefSeq: WP_001557458 NCBI
• UniProt: see SA1751

⊟Protein sequence

• MKFKSLITTTLALGVIASTGANFNTNEASAAAKPLDKSSSTLHHGHSNIQIPYTITVNGTSQNILSSLTFNKNQNISYKDIENKVKSVLYFNRGISDIDLRLSKQAEYTVHFKNGTKRVIDLKSGIYTADLINTSDIKAISVNVDTKKQPKDKAKANVQVPYTITVNGTSQNILSNLTFNKNQNISYKDLEGKVKSVLESNRGITDVDLRLSKQAKYTVNFKNGTKKVIDLKSGIYTANLINSSDIKSININVDTKKHIENKAKRNYQVPYSINLNGTSTNILSNLSFSNKPWTNYKNLTSQIKSVLKHDRGISEQDLKYAKKAYYTVYFKNGGKRILQLNSKNYTANLVHAKDVKRIEITVKTGTKAKADRYVPYTIAVNGTSTPILSKLKISNKQLISYKYLNDKVKSVLKSERGISDLDLKFAKQAKYTVYFKNGKKQVVNLKSDIFTPNLFSAKDIKKIDIDVKQYTKSKKK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: SaeR see SA1751

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications