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NCBI: 10-JUN-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_1917
- pan locus tag?: SAUPAN005009000
- symbol: map
- pan gene symbol?: eap/map
- synonym:
- product:
mapW: major histocompatibility complex II analog immune evasion protein, programmed frameshift
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: pseudogene
- locus tag: SAUSA300_1917
- symbol: map
- product:
- replicon: chromosome
- strand: -
- coordinates: 2082045..2084100
- length: 2056
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3912894 NCBI
- RefSeq:
- BioCyc:
- MicrobesOnline: 1293432 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
- 1
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2041ATGAAATTTAAGTCATTGATTACAACAACTTTAGCATTAGGTGTTTTAGCATCAACAGGT
GCAAACTTTAATAATAATGAAGCGTCTGCCGCAGCTAAGCCATTAGATAAATCATCAAGT
TCGTTACACCATGGATATTCTAAAGTCCATGTTCCATATGCAATCACTGTGAACGGTACA
AGCCAAAATATTTTATCAAGCTTAACATTTAATAAGAATCAAAATATTAGTTATAAAGAT
TTAGAGGATAGAGTTAAATCAGTTTTAAAATCAGACAGAGGTATTAGTGATATAGATTTA
AGACTATCGAAGCAAGCGAAATATACTGTTTACTTTAAAAATGGAACAAAGAAAGTTATC
GATTTGAAAGCAGGTATTTACACAGCTGATTTAATTAATACAAGTGAAATTAAAGCTATT
AATATTAACGTAGATACTAAAAAGCAAGTTGAAGATAAAAAGAAAGATAAAGCAAATTAC
CAAGTTCCATACACAATCACTGTGAACGGTACAAGCCAAAATATTTTATCAAACTTAACA
TTTAATAAGAATCAAAATATTAGTTACAAAGATTTAGAGGATAAAGTTAAATCAGTTTTA
GAATCAAATAGAGGTATTACCGATGTTGATTTAAGATTATCGAAGCAAGCGAAATATACA
GTTAATTTTAAAAATGGAACGAAGAAAGTTATCGATTTGAAATCAGGTATTTACACAGCG
AATTTAATAAATTCAAGTGATATTAAAAGTATCAATATTAACGTAGATACAAAAAAACAT
ATCGAAAATAAAGCTAAAAGAAACTATCAAGTTCCATATTCAATTAATTTAAATGGTACA
TCTACAAACATTTTATCGAATCTTTCATTTTCAAATAAACCTTGGACAAATTACAAAAAT
TTAACTAGTCAAATAAAATCAGTACTGAAGCATGATAGAGGTATTAGTGAACAAGATTTA
AAATATGCTAAGAAAGCTTATTATACTGTTTATTTTAAAAATGGTGGTAAAAGAATCTTA
CAGTTGAATTCAAAAAATTACACAGCAAACTTAGTTCATGCGAAAGATGTTAAGAGAATT
GAAATTACTGTTAAAACAGGAACTAAAGCGAAAGCAGACAGATATGTACCATACACAATT
GCAGTAAATGGCACATCAACACCAATTTTATCAGATTTAAAATTTACAGGTGACCCACGT
GTAGGCTACAAAGATATCTCTAAAAAAGTTAAATCAGTATTGAAGCATGATAGAGGTATC
GGGGAACGTGAATTAAAATATGCAAAAAAAGCAACTTACACAGTACATTTTAAAAATGGA
ACGAAAAAAGTGATTAACATAAATTCAAATATTAGCCAACTGAATCTGCTTTATGTTCAA
GATATTAAAAAGATAGATATTGATGTTAAAACAGGAACTAAAGCGAAAGCGGATAGCTAT
GTACCATATACAATTGCAGTAAATGGCACATCAACACCAATTTTATCAAAACTTAAAATT
TCGAATAAACAATTAATTAGTTACAAATATTTAAATGACAAAGTGAAATCTGTATTAAAA
AGTGAAAGAGGCATCAGTGATCTTGACTTAAAATTTGCGAAACAAGCAAAATATACAGTA
TATTTCAAAAATGGAAAGAAACAAGTAGTGAATTTAAAATCAGACATCTTTACACCTAAT
TTATTTAGTGCCAAAGATATTAAAAAGATTGATATTGATGTAAAACAATACACTAAATCA
AAAAAAAATAAATAAATCTAATAATGTGAAATTCCCAGTAACAATAAATAAATTTGAAAA
CATAGTTTCAAATGAATTTGTGTTCTATAATGCAAGCAAAATTACAATTAATGATTTAAG
TATAAAACTTAAATCAGCAATGGCAAATGATCAAGGGATAACTAAACATGACATAGGACT
TGCTGAACGCGCAGTGTATAAAGTGTATTTTAAAAATGGTTCGTCAAAATATGTAGACTT
AAAAACTGAGTATAAAGATGAAAGAGTATTTAAAGCAACTGACATTAAAAAGGTAGATAT
TGAACTTAAATTCTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_1917
- symbol: Map
- description:
- length:
- theoretical pI:
- theoretical MW:
- GRAVY:
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb:
- LocateP:
- SignalP:
- predicted transmembrane helices (TMHMM):
⊟Accession numbers[edit | edit source]
- GI:
- RefSeq:
- UniProt:
⊟Protein sequence[edit | edit source]
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: no polycistronic organisation predicted
⊟Regulation[edit | edit source]
- regulator: SaeR (activation) regulon
SaeR (TF) important in Virulence; compare RegPrecise for N315 [1]
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
There appear to be two alleles at this locus. One, the Map/Eap protein is exemplified by strain Newman; it is the original full-length eap gene. In contrast is the MapW protein. The mapW allele found in FPR3757, N315, Mu3, MW2 and COL is shorter with a poly-A region resulting in frame-slippage and a premature stop codon. Confusing the issue, characterization of the "Eap" protein and it's neutrophil protease, complement, extracellular matrix and eukaryotic cell surface receptor binding, appears to have used the shorter version encoded by mapW from Mu50. Therefore, although the gene nomenclature can use "eap" or "map" for the full-length protein and "mapW" for the shorter allele, the proper nomenclature for the encoded protein is unclear.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Qian Liu, Won-Sik Yeo, Taeok Bae
The SaeRS Two-Component System of Staphylococcus aureus.
Genes (Basel): 2016, 7(10);
[PubMed:27706107] [WorldCat.org] [DOI] (P e)
⊟Relevant publications[edit | edit source]
- ↑ Lawrence Y Lee, Yuko J Miyamoto, Bradley W McIntyre, Magnus Höök, Kirk W McCrea, Damien McDevitt, Eric L Brown
The Staphylococcus aureus Map protein is an immunomodulator that interferes with T cell-mediated responses.
J Clin Invest: 2002, 110(10);1461-71
[PubMed:12438444] [WorldCat.org] [DOI] (P p)Angus Buckling, James Neilson, Jodi Lindsay, Richard ffrench-Constant, Mark Enright, Nicholas Day, Ruth C Massey
Clonal distribution and phase-variable expression of a major histocompatibility complex analogue protein in Staphylococcus aureus.
J Bacteriol: 2005, 187(8);2917-9
[PubMed:15805539] [WorldCat.org] [DOI] (P p)Niamh Harraghy, Muzaffar Hussain, Axana Haggar, Triantafyllos Chavakis, Bhanu Sinha, Mathias Herrmann, Jan-Ingmar Flock
The adhesive and immunomodulating properties of the multifunctional Staphylococcus aureus protein Eap.
Microbiology (Reading): 2003, 149(Pt 10);2701-2707
[PubMed:14523103] [WorldCat.org] [DOI] (P p)Jordan L Woehl, Kasra X Ramyar, Benjamin B Katz, John K Walker, Brian V Geisbrecht
The structural basis for inhibition of the classical and lectin complement pathways by S. aureus extracellular adherence protein.
Protein Sci: 2017, 26(8);1595-1608
[PubMed:28512867] [WorldCat.org] [DOI] (I p)