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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1751 [new locus tag: SA_RS10060 ]
  • pan locus tag?: SAUPAN005009000
  • symbol: SA1751
  • pan gene symbol?: eap/map
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA1751 [new locus tag: SA_RS10060 ]
  • symbol: SA1751
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2003333..2004763
  • length: 1431
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGAAATTTAAGTCATTGATTACAACAACATTAGCATTAGGCGTTATAGCATCAACAGGA
    GCAAACTTTAATACTAACGAAGCATCTGCCGCAGCTAAGCCATTAGATAAATCATCAAGT
    ACATTACACCATGGACATTCTAACATCCAGATTCCATATACAATTACTGTGAACGGTACA
    AGCCAAAACATTTTATCAAGCTTAACATTTAATAAGAATCAAAATATTAGTTATAAAGAT
    ATAGAGAATAAAGTTAAATCAGTTTTATACTTTAATAGAGGTATTAGTGATATCGATTTA
    AGACTTTCAAAGCAAGCGGAATATACGGTTCATTTTAAAAATGGAACAAAAAGAGTTATC
    GATTTGAAATCAGGTATCTACACAGCTGACTTAATCAATACAAGTGACATTAAAGCTATC
    AGTGTTAACGTAGATACTAAAAAGCAACCTAAAGATAAAGCTAAAGCAAATGTTCAAGTG
    CCATATACAATCACAGTGAACGGCACAAGCCAAAACATTTTATCAAACCTAACATTTAAT
    AAAAATCAAAATATTAGTTACAAAGATTTAGAGGGTAAAGTTAAATCAGTTTTAGAATCA
    AATAGAGGTATTACTGATGTTGATTTAAGACTTTCGAAGCAAGCGAAATATACAGTTAAT
    TTTAAAAATGGAACGAAGAAAGTTATCGATTTGAAATCAGGTATTTACACAGCGAATTTA
    ATCAATTCAAGTGATATTAAAAGTATCAATATTAACGTAGATACAAAAAAACATATCGAA
    AATAAAGCTAAAAGAAACTATCAAGTTCCATATTCAATTAATCTAAATGGTACATCTACA
    AACATTTTATCGAATCTTTCATTTTCAAATAAACCTTGGACAAATTACAAAAATTTAACT
    AGTCAAATAAAATCAGTACTGAAGCATGATAGAGGTATTAGTGAACAAGATTTAAAATAT
    GCTAAGAAAGCTTATTATACTGTTTATTTTAAAAATGGTGGTAAAAGAATCTTACAGTTA
    AATTCAAAAAATTACACAGCAAACTTAGTTCATGCGAAAGATGTTAAGAGAATTGAAATT
    ACTGTTAAAACAGGAACTAAAGCGAAAGCAGACAGATATGTACCATACACAATTGCAGTA
    AATGGCACATCAACACCAATTTTATCAAAACTAAAAATTTCGAATAAACAATTAATTAGT
    TACAAATATTTAAACGACAAAGTGAAATCTGTATTAAAAAGTGAAAGAGGTATCAGTGAT
    CTTGACTTAAAATTTGCGAAACAAGCAAAATATACAGTATATTTCAAAAATGGAAAGAAA
    CAAGTAGTGAATTTAAAATCAGACATCTTTACACCTAATTTATTTAGTGCCAAAGATATT
    AAAAAGATTGATATTGATGTAAAACAATACACTAAATCAAAAAAAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1431

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA1751 [new locus tag: SA_RS10060 ]
  • symbol: SA1751
  • description: hypothetical protein
  • length: 476
  • theoretical pI: 10.5909
  • theoretical MW: 53376.8
  • GRAVY: -0.552731

⊟Function[edit | edit source]

  • TIGRFAM:
    modification target Cys-rich peptide, AC3_0185 family (TIGR04118; HMM-score: 12.5)
  • TheSEED  :
    • Extracellular adherence protein of broad specificity Eap/Map
    Virulence, Disease and Defense Adhesion Adhesins in Staphylococcus  Extracellular adherence protein of broad specificity Eap/Map
  • PFAM:
    Ant-toxin_C (CL0386) MAP; MAP domain (PF03642; HMM-score: 466)
    and 10 more
    Tudor (CL0049) LBR_tudor; Lamin-B receptor of TUDOR domain (PF09465; HMM-score: 20.8)
    no clan defined DUF3553; Protein of unknown function (DUF3553) (PF12073; HMM-score: 18.5)
    Homing_endonuc (CL0324) Hom_end; Homing endonuclease (PF05204; HMM-score: 17.9)
    Sm-like (CL0527) LSM14; Scd6-like Sm domain (PF12701; HMM-score: 14)
    no clan defined GAPES3; Gammaproteobacterial periplasmic sensor domain (PF17154; HMM-score: 13.6)
    HMA; Heavy-metal-associated domain (PF00403; HMM-score: 11.7)
    Trefoil (CL0066) DUF569; Domain of unknown function (DUF569) (PF04601; HMM-score: 11.7)
    no clan defined Ribosomal_S21e; Ribosomal protein S21e (PF01249; HMM-score: 11.2)
    Cupin (CL0029) VIT_2; Vault protein inter-alpha-trypsin domain (PF13757; HMM-score: 8.3)
    no clan defined Neisseria_TspB; Neisseria meningitidis TspB protein (PF05616; HMM-score: 7.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 0
  • LocateP: Secretory(released) (with CS)
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: -0.17
    • Signal peptide possibility: 1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: NEASAAAK
  • SignalP: Signal peptide SP(Sec/SPI) length 30 aa
    • SP(Sec/SPI): 0.89345
    • TAT(Tat/SPI): 0.004555
    • LIPO(Sec/SPII): 0.096731
    • Cleavage Site: CS pos: 30-31. ASA-AA. Pr: 0.5840
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKFKSLITTTLALGVIASTGANFNTNEASAAAKPLDKSSSTLHHGHSNIQIPYTITVNGTSQNILSSLTFNKNQNISYKDIENKVKSVLYFNRGISDIDLRLSKQAEYTVHFKNGTKRVIDLKSGIYTADLINTSDIKAISVNVDTKKQPKDKAKANVQVPYTITVNGTSQNILSNLTFNKNQNISYKDLEGKVKSVLESNRGITDVDLRLSKQAKYTVNFKNGTKKVIDLKSGIYTANLINSSDIKSININVDTKKHIENKAKRNYQVPYSINLNGTSTNILSNLSFSNKPWTNYKNLTSQIKSVLKHDRGISEQDLKYAKKAYYTVYFKNGGKRILQLNSKNYTANLVHAKDVKRIEITVKTGTKAKADRYVPYTIAVNGTSTPILSKLKISNKQLISYKYLNDKVKSVLKSERGISDLDLKFAKQAKYTVYFKNGKKQVVNLKSDIFTPNLFSAKDIKKIDIDVKQYTKSKKK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: SaeR (activation) regulon
    SaeR(TF)important in Virulence; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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