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PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_001974
  • pan locus tag?: SAUPAN005009000
  • symbol: eap
  • pan gene symbol?: eap/map
  • synonym:
  • product: extracellular adherence protein Eap/Map

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_001974
  • symbol: eap
  • product: extracellular adherence protein Eap/Map
  • replicon: chromosome
  • strand: -
  • coordinates: 1985178..1986926
  • length: 1749
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGAAATTTAAGTCATTGATTACAACAACATTAGCATTAGGCGTTATAGCATCAACAGGA
    GCAAACTTTAATACTAACGAAGCATCTGCCGCAGCTAAGCCATTAGATAAATCATCAAGT
    ACATTACACCATGGACATTCTAACATCCAGATTCCATATACAATTACTGTGAACGGCACA
    AGCCAAAACATTTTATCAAGCTTAACATTTAATAAGAATCAAAATATTAGTTATAAAGAT
    ATAGAGAATAAAGTTAAATCAGTTTTATACTTTAATAGAGGTATTAGTGATATCGATTTA
    AGACTTTCAAAGCAAGCGGAATATACGGTTCATTTTAAAAATGGAACAAAAAGAGTTATC
    GATTTGAAATCAGGTATCTACACAGCTGACTTAATCAATACAAGTGACATTAAAGCTATC
    AGTGTTAACGTAGATACTAAAAAGCAACCTAAAGATAAAGCTAAAGCAAATGTTCAAGTG
    CCATATACAATCACAGTGAACGGCACAAGCCAAAACATTTTATCAAACCTAACATTTAAT
    AAAAATCAAAATATTAGTTACAAAGATTTAGAGGGTAAAGTTAAATCAGTTTTAGAATCA
    AATAGAGGTATTACTGATGTTGATTTAAGACTTTCGAAGCAAGCGAAATATACAGTTAAT
    TTTAAAAATGGAACGAAGAAAGTTATAGATTTGAAATCAGGTATTTACACAGCGAATTTA
    ATCAATTCAAGTGATATTAAAAGTATCAATATTAACGTAGATACAAAAAAACATATCGAA
    AATAAAGCTAAAAGAAACTATCAAGTTCCATATTCAATTAATTTAAATGGTACATCTACA
    AACATTTTATCGAATCTTTCATTTTCAAATAAACCTTGGACAAATTACAAAAATTTAACT
    AGTCAAATAAAATCAGTACTGAAGCATGATAGAGGTATTAGTGAACAAGATTTAAAATAT
    GCTAAGAAAGCTTATTATACTGTTTATTTTAAAAATGGTGGTAAAAGAATCTTACAGTTA
    AATTCAAAAAATTACACAGCAAACTTAGTTCATGCGAAAGATGTTAAGAGAATTGAAATT
    ACTGTTAAAACAGGAACTAAAGCGAAAGCAGACAGATATGTACCATACACAATTGCAGTA
    AATGGCACATCAACACCAATTTTATCAAAACTAAAAATTTCGAATAAACAATTAATTAGT
    TACAAATATTTAAACGACAAAGTGAAATCTGTATTAAAAAGTGAAAGAGATATCAGTGAT
    CTTGACTTAAAATTTGCGAAACAAGCAAAATATACAGTATATTTCAAAAATGGAAAGAAA
    CAAGTAGTGAATTTAAAATCAGACATCTTTACACCTAATTTATTTAGTGCCAAAGATATT
    AAAAAGATAGATATTGATGTTAAAACAGGGTCAAAAGCGAAAGCGGATAGCTATGTACCA
    TATACAATTGCAGTAAATGGCACATCAACACCAATTTTATCAAAACTAAAAATTTCGAAT
    AAACAATTAATTAGTTACAAATATTTAAATGACAAAGTGAAATCTGTATTAAAAAGTGAA
    AGAGGCATCAGTGATCTTGACTTAAAATTTGCGAAACAAGCAAAATATACAGTATATTTC
    AAAAATGGAAAGAAACAAGTAGTGAATTTAAAATCAGACATCTTTACACCTAATTTATTT
    AGTGCCAAAGATATTAAAAAGATAGATATTGATGTAAAACAATACACTAAATCAAAAAAA
    AATAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1749

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_001974
  • symbol: Eap
  • description: extracellular adherence protein Eap/Map
  • length: 582
  • theoretical pI: 10.5718
  • theoretical MW: 65295.5
  • GRAVY: -0.536254

⊟Function[edit | edit source]

  • TIGRFAM:
    modification target Cys-rich peptide, AC3_0185 family (TIGR04118; HMM-score: 20.2)
  • TheSEED: data available for COL, N315, NCTC8325, Newman
  • PFAM:
    no clan defined MAP; MAP domain (PF03642; HMM-score: 605.2)
    and 16 more
    SH3 (CL0010) DUF7141; Domain of unknown function (DUF7141) (PF23614; HMM-score: 28)
    DUF3553; Protein of unknown function (DUF3553) (PF12073; HMM-score: 24.7)
    no clan defined APS-reductase_C; Adenosine-5'-phosphosulfate reductase beta subunit (PF12139; HMM-score: 24.7)
    PLP_aminotran (CL0061) ComK; ComK protein (PF06338; HMM-score: 24.5)
    SH3 (CL0010) Tudor_3; DNA repair protein Crb2 Tudor domain (PF18115; HMM-score: 18.7)
    no clan defined M_C; Protein M, C-terminal (PF22806; HMM-score: 18.5)
    Homing_endonuc (CL0324) Hom_end; Homing endonuclease (PF05204; HMM-score: 17.8)
    E-set (CL0159) DUF5001; Ig-like domain (PF16392; HMM-score: 17.5)
    Cache (CL0165) GAPES3; Gammaproteobacterial periplasmic sensor domain (PF17154; HMM-score: 14.9)
    E-set (CL0159) aRib; Atypical Rib domain (PF18938; HMM-score: 14.2)
    DUF5780; Family of unknown function (DUF5780) (PF19092; HMM-score: 14.2)
    no clan defined TFX_C; DNA_binding protein, TFX, C-term (PF14601; HMM-score: 11.9)
    Trefoil (CL0066) DUF569; Beta-trefoil domain of unknown function (DUF569) (PF04601; HMM-score: 11.6)
    SH3 (CL0010) LSM14; Scd6-like Sm domain (PF12701; HMM-score: 11.3)
    no clan defined Frataxin_Cyay; Frataxin-like domain (PF01491; HMM-score: 9.6)
    HMA (CL0704) HMA; Heavy-metal-associated domain (PF00403; HMM-score: 7.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 0
  • DeepLocPro: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.0001
    • Cell wall & surface Score: 0.4395
    • Extracellular Score: 0.5604
  • LocateP:
  • SignalP: Signal peptide SP(Sec/SPI) length 30 aa
    • SP(Sec/SPI): 0.89345
    • TAT(Tat/SPI): 0.004555
    • LIPO(Sec/SPII): 0.096731
    • Cleavage Site: CS pos: 30-31. ASA-AA. Pr: 0.5840
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MKFKSLITTTLALGVIASTGANFNTNEASAAAKPLDKSSSTLHHGHSNIQIPYTITVNGTSQNILSSLTFNKNQNISYKDIENKVKSVLYFNRGISDIDLRLSKQAEYTVHFKNGTKRVIDLKSGIYTADLINTSDIKAISVNVDTKKQPKDKAKANVQVPYTITVNGTSQNILSNLTFNKNQNISYKDLEGKVKSVLESNRGITDVDLRLSKQAKYTVNFKNGTKKVIDLKSGIYTANLINSSDIKSININVDTKKHIENKAKRNYQVPYSINLNGTSTNILSNLSFSNKPWTNYKNLTSQIKSVLKHDRGISEQDLKYAKKAYYTVYFKNGGKRILQLNSKNYTANLVHAKDVKRIEITVKTGTKAKADRYVPYTIAVNGTSTPILSKLKISNKQLISYKYLNDKVKSVLKSERDISDLDLKFAKQAKYTVYFKNGKKQVVNLKSDIFTPNLFSAKDIKKIDIDVKTGSKAKADSYVPYTIAVNGTSTPILSKLKISNKQLISYKYLNDKVKSVLKSERGISDLDLKFAKQAKYTVYFKNGKKQVVNLKSDIFTPNLFSAKDIKKIDIDVKQYTKSKKNK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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