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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_RS02510 [old locus tag: SAUSA300_0467 ]
  • pan locus tag?: SAUPAN002227000
  • symbol: SAUSA300_RS02510
  • pan gene symbol?: metS
  • synonym:
  • product: methionine--tRNA ligase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_RS02510 [old locus tag: SAUSA300_0467 ]
  • symbol: SAUSA300_RS02510
  • product: methionine--tRNA ligase
  • replicon: chromosome
  • strand: +
  • coordinates: 525789..527762
  • length: 1974
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGCTAAAGAAACATTTTATATAACAACCCCAATATACTATCCTAGTGGGAATTTACAT
    ATAGGACATGCATATTCTACAGTGGCTGGAGATGTTATTGCAAGATATAAGAGAATGCAA
    GGATATGATGTTCGCTATTTGACTGGAACGGATGAACACGGTCAAAAAATTCAAGAAAAA
    GCTCAAAAATCTGGTAAGACAGAAATTGAATATTTGGATGAGATGATTGCTGGAATTAAA
    CAATTGTGGGCTAAGCTTGAAATTTCAAATGATGATTTTATCAGAACAACTGAAGAACGT
    CATAAACATGTCGTTGAGCAAGTGTTTGAACGTTTATTAAAGCAAGGTGATATCTATTTA
    GGTGAATATGAAGGTTGGTATTCTGTTCCGGATGAAACATACTATACAGAGTCACAATTA
    GTAGACCCACAATACGAAAACGGTAAAATTATTGGTGGCAAAAGTCCAGATTCTGGACAC
    GAAGTTGAACTAGTTAAAGAAGAAAGTTATTTCTTTAATATTAGTAAATATACAGACCGT
    TTATTAGAGTTCTATGACCAAAATCCAGATTTTATACAACCACCATCAAGAAAAAATGAA
    ATGATTAACAACTTCATTAAACCAGGACTTGCTGATTTAGCTGTTTCTCGTACATCATTT
    AACTGGGGTGTCCATGTTCCGTCTAATCCAAAACATGTTGTTTATGTTTGGATTGATGCG
    TTAGTTAACTATATTTCAGCATTAGGCTATTTATCAGATGATGAGTCACTATTTAACAAA
    TACTGGCCAGCAGATATTCATTTAATGGCTAAGGAAATTGTGCGATTCCACTCAATTATT
    TGGCCTATTTTATTGATGGCATTAGACTTACCGTTACCTAAAAAAGTCTTTGCACATGGT
    TGGATTTTGATGAAAGATGGAAAAATGAGTAAATCTAAAGGTAATGTCGTAGACCCTAAT
    ATTTTAATTGATCGCTATGGTTTAGATGCTACACGTTATTATCTAATGCGTGAATTACCA
    TTTGGTTCAGATGGCGTATTTACACCTGAAGCATTTGTTGAGCGTACAAATTTCGATCTA
    GCAAATGACTTAGGTAACTTAGTAAACCGTACGATTTCTATGGTTAATAAGTACTTTGAT
    GGCGAATTACCAGCGTATCAAGGTCCACTTCATGAATTAGATGAAGAAATGGAAGCTATG
    GCTTTAGAAACAGTGAAAAGCTACACTGAAAGCATGGAAAGTTTGCAATTTTCTGTGGCA
    TTATCTACGGTATGGAAGTTTATTAGTAGAACGAATAAGTATATTGACGAAACAACGCCT
    TGGGTATTAGCTAAGGACGATAGCCAAAAAGATATGTTAGGCAATGTAATGGCTCACTTA
    GTTGAAAATATTCGTTATGCAGCTGTATTATTACGTCCATTCTTAACACATGCGCCGAAA
    GAGATTTTTGAACAATTGAACATTAACAATCCTCAATTTATGGAATTTAGTAGTTTAGAG
    CAATATGGTGTGCTTAATGAGTCAATTATGGTTACTGGGCAACCTAAACCTATTTTCCCA
    AGATTGGATAGCGAAGCGGAAATTGCATATATCAAAGAATCAATGCAACCGCCTGCTACT
    AAAGAGGAAAAAGAAGAGATTCCTAGCAAACCTCAAATTGATATTAAAGACTTTGATAAA
    GTTGAAATTAAGGCAGCAACGATTATTGATGCTGAACATGTTAAGAAGTCAGATAAGCTT
    TTAAAAATTCAAGTAGACTTAGATTCTGAACAAAGACAAATTGTATCAGGAATTGCCAAA
    TTCTATACACCAGATGATATTATTGGTAAAAAAGTAGCAGTTGTTACTAACCTGAAACCA
    GCTAAATTAATGGGACAAAAATCTGAAGGTATGATATTATCTGCTGAAAAAGATGGTGTA
    TTAACCTTAGTAAGTTTACCAAGTGCAATTCCAAATGGTGCAGTGATTAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1974

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_RS02510 [old locus tag: SAUSA300_0467 ]
  • symbol: SAUSA300_RS02510
  • description: methionine--tRNA ligase
  • length: 657
  • theoretical pI: 4.94244
  • theoretical MW: 74901
  • GRAVY: -0.342618

⊟Function[edit | edit source]

  • reaction:
    EC 6.1.1.10?  ExPASy
    Methionine--tRNA ligase ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met)
  • TIGRFAM:
    Genetic information processing Protein synthesis tRNA aminoacylation methionine--tRNA ligase (TIGR00398; EC 6.1.1.10; HMM-score: 564.8)
    and 12 more
    Genetic information processing Protein synthesis tRNA aminoacylation valine--tRNA ligase (TIGR00422; EC 6.1.1.9; HMM-score: 149.2)
    Genetic information processing Protein synthesis tRNA aminoacylation methionine--tRNA ligase, beta subunit (TIGR00399; EC 6.1.1.10; HMM-score: 138.6)
    Genetic information processing Protein synthesis tRNA aminoacylation leucine--tRNA ligase (TIGR00396; EC 6.1.1.4; HMM-score: 115.3)
    Genetic information processing Protein synthesis tRNA aminoacylation isoleucine--tRNA ligase (TIGR00392; EC 6.1.1.5; HMM-score: 112.8)
    Genetic information processing Protein synthesis tRNA aminoacylation leucine--tRNA ligase (TIGR00395; EC 6.1.1.4; HMM-score: 81)
    Genetic information processing Protein synthesis tRNA aminoacylation cysteine--tRNA ligase (TIGR00435; EC 6.1.1.16; HMM-score: 62.3)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking export-related chaperone protein CsaA (TIGR02222; HMM-score: 38.7)
    Genetic information processing Protein fate Protein folding and stabilization export-related chaperone protein CsaA (TIGR02222; HMM-score: 38.7)
    Genetic information processing Protein synthesis tRNA aminoacylation phenylalanine--tRNA ligase, beta subunit (TIGR00472; EC 6.1.1.20; HMM-score: 27.2)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Glutathione and analogs cysteine--1-D-myo-inosityl 2-amino-2-deoxy-alpha-D-glucopyranoside ligase (TIGR03447; EC 6.3.1.13; HMM-score: 26.4)
    Genetic information processing Protein synthesis tRNA aminoacylation arginine--tRNA ligase (TIGR00456; EC 6.1.1.19; HMM-score: 24)
    Genetic information processing Protein synthesis tRNA aminoacylation lysine--tRNA ligase (TIGR00467; EC 6.1.1.6; HMM-score: 16.3)
  • TheSEED: see SAUSA300_0467
  • PFAM:
    HUP (CL0039) tRNA-synt_1g; tRNA synthetases class I (M) (PF09334; HMM-score: 387)
    and 10 more
    tRNA-synt_1; tRNA synthetases class I (I, L, M and V) (PF00133; HMM-score: 105.5)
    OB (CL0021) tRNA_bind; Putative tRNA binding domain (PF01588; HMM-score: 89.1)
    HUP (CL0039) tRNA-synt_1e; tRNA synthetases class I (C) catalytic domain (PF01406; HMM-score: 56.5)
    DALR (CL0258) Anticodon_1; Anticodon-binding domain of tRNA (PF08264; HMM-score: 36.3)
    HUP (CL0039) tRNA-synt_1f; tRNA synthetases class I (K) (PF01921; HMM-score: 25.4)
    Pilus (CL0327) PilM; PilM (PF07419; HMM-score: 14.6)
    no clan defined ARL2_Bind_BART; The ARF-like 2 binding protein BART (PF11527; HMM-score: 12.9)
    HUP (CL0039) tRNA-synt_1d; tRNA synthetases class I (R) (PF00750; HMM-score: 12.7)
    no clan defined T4_neck-protein; Virus neck protein (PF11649; HMM-score: 10.9)
    HUP (CL0039) tRNA-synt_1c; tRNA synthetases class I (E and Q), catalytic domain (PF00749; HMM-score: 10.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.017259
    • TAT(Tat/SPI): 0.001172
    • LIPO(Sec/SPII): 0.001252
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MAKETFYITTPIYYPSGNLHIGHAYSTVAGDVIARYKRMQGYDVRYLTGTDEHGQKIQEKAQKSGKTEIEYLDEMIAGIKQLWAKLEISNDDFIRTTEERHKHVVEQVFERLLKQGDIYLGEYEGWYSVPDETYYTESQLVDPQYENGKIIGGKSPDSGHEVELVKEESYFFNISKYTDRLLEFYDQNPDFIQPPSRKNEMINNFIKPGLADLAVSRTSFNWGVHVPSNPKHVVYVWIDALVNYISALGYLSDDESLFNKYWPADIHLMAKEIVRFHSIIWPILLMALDLPLPKKVFAHGWILMKDGKMSKSKGNVVDPNILIDRYGLDATRYYLMRELPFGSDGVFTPEAFVERTNFDLANDLGNLVNRTISMVNKYFDGELPAYQGPLHELDEEMEAMALETVKSYTESMESLQFSVALSTVWKFISRTNKYIDETTPWVLAKDDSQKDMLGNVMAHLVENIRYAAVLLRPFLTHAPKEIFEQLNINNPQFMEFSSLEQYGVLNESIMVTGQPKPIFPRLDSEAEIAYIKESMQPPATKEEKEEIPSKPQIDIKDFDKVEIKAATIIDAEHVKKSDKLLKIQVDLDSEQRQIVSGIAKFYTPDDIIGKKVAVVTNLKPAKLMGQKSEGMILSAEKDGVLTLVSLPSAIPNGAVIK

⊟Experimental data[edit | edit source]

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.000 1.001 1.002 1.003 1.004 1.005 1.006 1.007 1.008 1.009 1.010 1.011 1.012 1.013 1.014 1.015 1.016 1.017 1.018 1.019 1.020 1.021 1.022 1.023 1.024 1.025 1.026 1.027 1.028 1.029 1.030 1.031 1.032 1.033 1.034 1.035 1.036 1.037 1.038 1.039 1.040 1.041 1.042 1.043 1.044 1.045 1.046 1.047 1.048 1.049 1.050 1.051 1.052 1.053 1.054 1.055 1.056 1.057 1.058 1.059 1.060 1.061 1.062 1.063 1.064 1.065 1.066 1.067 1.068 1.069 1.070 1.071 1.072 1.073 1.074 1.075 1.076 1.077 1.078 1.079 1.080 1.081 1.082 1.083 1.084 1.085 1.086 1.087 1.088 1.089 1.090 1.091 1.092 1.093 1.094 1.095 1.096 1.097 1.098 1.099 1.100 1.101 1.102 1.103 1.104 1.105 1.106 1.107 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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