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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_2519 [new locus tag: SAUSA300_RS13980 ]
  • pan locus tag?: SAUPAN006259000
  • symbol: SAUSA300_2519
  • pan gene symbol?: —
  • synonym:
  • product: putative cobalamin synthesis protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_2519 [new locus tag: SAUSA300_RS13980 ]
  • symbol: SAUSA300_2519
  • product: putative cobalamin synthesis protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2721720..2722610
  • length: 891
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGAAAATAGTTATTATAGGTGGGTTTTTAGGTGGCGGTAAAACGACTGTCTTAAATCAT
    TTGCTCGCTGAATCATTAAAGGAATCGCTGAAACCAGCAGTCATCATGAATGAATTTGGG
    AAAATGAGTGTTGATGGTGCCTTAGTATCTGAAGACATACCTTTAAGTGAACTGACAGAG
    GGGTGTATCTGTTGTGCAATGAAAGCAGATGTATCAGAACAGTTACATCAATTATATTTA
    AAAGAGCAACCAGACATTGTATTTATTGAATGTAGTGGGATTGCAGAACCGGTCTCTGTC
    TTAGATGCTTGTTTAACGCCTATTTTAGCTCCGTTTACAACAATTACACATATGATTGGT
    GTAATAGACGCAAGCATGTATAAACACATTAAATCATTCCCTAAAGACATCCAAGGCTTA
    TTTTATGAGCAATTAGCATATTGTTCTGTCTTATTTGTTAATAAAATAGATTCAGCAGAT
    GTTGAAACAACGAGCAAACTATTGAAAGATTTAGAAGTTATTAACCCAGAGGCCGATATA
    CAAGTCGGTATGCATGGCAGCGTCACTTTGCCAATATCAGTTAGACAAATGACAGCAACT
    TCTGACAATAAACATAAGTCTTTACATCAAATGATTAATCATCAATTTGTGCAATCACCA
    GTCAAATGTACTAAAGCAGAGTTTATAAAACGTTTAGCATGCCTTCCGTCTCATATTTAT
    AGGTTGAAAGGGTTTATGACATTTGAAGACACCGCACATACGTATCTCATTCAATTTACA
    CAAGGACAATATGAATTAACACCTGTAGCATTTTCAAAAAAAGTGCCAGAATATTTGGTG
    CTTATCGGAAAGGGTATTTCAAAAGAAGACTATCAATGTTTGGAACAGTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    891

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_2519 [new locus tag: SAUSA300_RS13980 ]
  • symbol: SAUSA300_2519
  • description: putative cobalamin synthesis protein
  • length: 296
  • theoretical pI: 5.98589
  • theoretical MW: 32835.1
  • GRAVY: 0.117568

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin cobalamin biosynthesis protein CobW (TIGR02475; HMM-score: 83.2)
    and 2 more
    Genetic information processing Protein fate Protein modification and repair hydrogenase accessory protein HypB (TIGR00073; HMM-score: 24.8)
    Unknown function General small GTP-binding protein domain (TIGR00231; HMM-score: 17.4)
  • TheSEED  :
    • Putative metal chaperone, involved in Zn homeostasis, GTPase of COG0523 family
    COG0523-Histidine  Putative metal chaperone, involved in Zn homeostasis, GTPase of COG0523 family
  • PFAM:
    P-loop_NTPase (CL0023) cobW; CobW/HypB/UreG, nucleotide-binding domain (PF02492; HMM-score: 155.4)
    and 7 more
    no clan defined CobW_C; Cobalamin synthesis protein cobW C-terminal domain (PF07683; HMM-score: 36.9)
    P-loop_NTPase (CL0023) Arf; ADP-ribosylation factor family (PF00025; HMM-score: 21.8)
    AAA_28; AAA domain (PF13521; HMM-score: 14.7)
    AAA_18; AAA domain (PF13238; HMM-score: 12.3)
    AAA_22; AAA domain (PF13401; HMM-score: 12.1)
    Ras; Ras family (PF00071; HMM-score: 11.9)
    ArgK; ArgK protein (PF03308; HMM-score: 11.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helix: 1
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.048081
    • TAT(Tat/SPI): 0.000366
    • LIPO(Sec/SPII): 0.002087
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKIVIIGGFLGGGKTTVLNHLLAESLKESLKPAVIMNEFGKMSVDGALVSEDIPLSELTEGCICCAMKADVSEQLHQLYLKEQPDIVFIECSGIAEPVSVLDACLTPILAPFTTITHMIGVIDASMYKHIKSFPKDIQGLFYEQLAYCSVLFVNKIDSADVETTSKLLKDLEVINPEADIQVGMHGSVTLPISVRQMTATSDNKHKSLHQMINHQFVQSPVKCTKAEFIKRLACLPSHIYRLKGFMTFEDTAHTYLIQFTQGQYELTPVAFSKKVPEYLVLIGKGISKEDYQCLEQ

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: Zur* (repression) regulon
    Zur*(TF)important in Zinc homeostasis; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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