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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_RS13980 [old locus tag: SAUSA300_2519 ]
- pan locus tag?: SAUPAN006259000
- symbol: SAUSA300_RS13980
- pan gene symbol?: —
- synonym:
- product: GTP-binding protein
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_RS13980 [old locus tag: SAUSA300_2519 ]
- symbol: SAUSA300_RS13980
- product: GTP-binding protein
- replicon: chromosome
- strand: -
- coordinates: 2721720..2722610
- length: 891
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Location: NC_007793 (2721720..2722610) NCBI
- BioCyc: SAUSA300_RS13980 BioCyc
- MicrobesOnline: see SAUSA300_2519
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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841ATGAAAATAGTTATTATAGGTGGGTTTTTAGGTGGCGGTAAAACGACTGTCTTAAATCAT
TTGCTCGCTGAATCATTAAAGGAATCGCTGAAACCAGCAGTCATCATGAATGAATTTGGG
AAAATGAGTGTTGATGGTGCCTTAGTATCTGAAGACATACCTTTAAGTGAACTGACAGAG
GGGTGTATCTGTTGTGCAATGAAAGCAGATGTATCAGAACAGTTACATCAATTATATTTA
AAAGAGCAACCAGACATTGTATTTATTGAATGTAGTGGGATTGCAGAACCGGTCTCTGTC
TTAGATGCTTGTTTAACGCCTATTTTAGCTCCGTTTACAACAATTACACATATGATTGGT
GTAATAGACGCAAGCATGTATAAACACATTAAATCATTCCCTAAAGACATCCAAGGCTTA
TTTTATGAGCAATTAGCATATTGTTCTGTCTTATTTGTTAATAAAATAGATTCAGCAGAT
GTTGAAACAACGAGCAAACTATTGAAAGATTTAGAAGTTATTAACCCAGAGGCCGATATA
CAAGTCGGTATGCATGGCAGCGTCACTTTGCCAATATCAGTTAGACAAATGACAGCAACT
TCTGACAATAAACATAAGTCTTTACATCAAATGATTAATCATCAATTTGTGCAATCACCA
GTCAAATGTACTAAAGCAGAGTTTATAAAACGTTTAGCATGCCTTCCGTCTCATATTTAT
AGGTTGAAAGGGTTTATGACATTTGAAGACACCGCACATACGTATCTCATTCAATTTACA
CAAGGACAATATGAATTAACACCTGTAGCATTTTCAAAAAAAGTGCCAGAATATTTGGTG
CTTATCGGAAAGGGTATTTCAAAAGAAGACTATCAATGTTTGGAACAGTAG60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_RS13980 [old locus tag: SAUSA300_2519 ]
- symbol: SAUSA300_RS13980
- description: GTP-binding protein
- length: 296
- theoretical pI: 5.98589
- theoretical MW: 32835.1
- GRAVY: 0.117568
⊟Function[edit | edit source]
- TIGRFAM: Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin cobalamin biosynthesis protein CobW (TIGR02475; HMM-score: 83.2)and 2 moreProtein fate Protein modification and repair hydrogenase accessory protein HypB (TIGR00073; HMM-score: 24.8)Unknown function General small GTP-binding protein domain (TIGR00231; HMM-score: 17.4)
- TheSEED: see SAUSA300_2519
- PFAM: P-loop_NTPase (CL0023) cobW; CobW/HypB/UreG, nucleotide-binding domain (PF02492; HMM-score: 155.4)and 7 moreno clan defined CobW_C; Cobalamin synthesis protein cobW C-terminal domain (PF07683; HMM-score: 36.9)P-loop_NTPase (CL0023) Arf; ADP-ribosylation factor family (PF00025; HMM-score: 21.8)AAA_28; AAA domain (PF13521; HMM-score: 14.7)AAA_18; AAA domain (PF13238; HMM-score: 12.3)AAA_22; AAA domain (PF13401; HMM-score: 12.1)Ras; Ras family (PF00071; HMM-score: 11.9)ArgK; ArgK protein (PF03308; HMM-score: 11.3)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helix: 1
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.048081
- TAT(Tat/SPI): 0.000366
- LIPO(Sec/SPII): 0.002087
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
- GI: 446629173 NCBI
- RefSeq: WP_000706519 NCBI
- UniProt: see SAUSA300_2519
⊟Protein sequence[edit | edit source]
- MKIVIIGGFLGGGKTTVLNHLLAESLKESLKPAVIMNEFGKMSVDGALVSEDIPLSELTEGCICCAMKADVSEQLHQLYLKEQPDIVFIECSGIAEPVSVLDACLTPILAPFTTITHMIGVIDASMYKHIKSFPKDIQGLFYEQLAYCSVLFVNKIDSADVETTSKLLKDLEVINPEADIQVGMHGSVTLPISVRQMTATSDNKHKSLHQMINHQFVQSPVKCTKAEFIKRLACLPSHIYRLKGFMTFEDTAHTYLIQFTQGQYELTPVAFSKKVPEYLVLIGKGISKEDYQCLEQ
⊟Experimental data[edit | edit source]
- experimentally validated:
- protein localization:
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator: Zur* see SAUSA300_2519
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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