PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_2520 [new locus tag: SAUSA300_RS13985 ]
• pan locus tag^?: SAUPAN006261000
• symbol: SAUSA300_2520
• pan gene symbol^?: —
• synonym:
• product: transporter gate domain-containing protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_2520 [new locus tag: SAUSA300_RS13985 ]
• symbol: SAUSA300_2520
• product: transporter gate domain-containing protein
• replicon: chromosome
• strand: -
• coordinates: 2722708..2724027
• length: 1320
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3912986 NCBI
• RefSeq: YP_495154 NCBI
• BioCyc: see SAUSA300_RS13985
• MicrobesOnline: 1294035 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGAGAACTGGGCATTATACGCCCATACCTAATGAACCTCATTATTTGGTTATTAGTCAT
  GCGGATAAACTTACCGCAACAGAAAAAGCGAAATTAAGATTATTAATCATAAAACAGAAA
  TTAGATATTTCATTGGCAGAAAGTGTAGTTTCTTCGCCTATAGCGAGTGAACATGTGATA
  GAACAATTGACACTATTTCAACATGAGCGACGACATTTAAGACCTAAAATAAGTGCGACA
  TTTTTAGCCTGGTTGTTGATATTTTTAATGTTTGCATTGCCAATCGGTATCGCTTATCAA
  TTTTCAGATTGGTTTCAAAATCAGTATGTGTCAGCATGGATAGAATATTTAACTCAAACA
  ACATTGCTCAATCACGATATATTACAGCATATATTATTTGGTGATTATGGTGTGCTATCA
  CTTGGAACATATTCGCTCGTATGGGCATTGCCGGTTGTAATATTGATTAGTTTATCAACT
  GCTATAATTGATCAAACAGGACTCAAATCATGGATGATATGGGCAATTGAACCGTCAATG
  TTATGGATAGGATTACAAGGTAATGATATCGTGCCACTATTAGAAGGGTTTGGATGTAAT
  GCAGCAGCTATTTCACAAGCAGCACACCAATGCCATACCTGCACGAAGACACAGTGTATG
  AGTTTAATAAGCTTTGGTAGTTCTTGTAGTTATCAAATAGGTGCGACATTATCTATTTTT
  AGTGTAGCTGGAAAGTCATGGCTATTTATGCCGTACTTAATATTAGTACTTTTAGGTGGC
  ATCTTACATAATAGGATATGGTTGAAAAAGAATGATCAACAACTTAGCGTTCCGCTACCT
  TATGATAGGCAATTACATATGCCAAATATACGTCAAATGTTGCTACAAATGTGGCAAAAT
  ATACAAATGTTTATCGTTCAAGCGCTACCTATTTTTATCACAATCTGTCTTATTGTTAGT
  ATTTTATCACTAACGCCAATTTTGAATGTTTTATCACAAATATTTACACCTATATTATCG
  TTATTAGGCATCTCGTCAGAATTGTCACCAGGGATTTTATTTTCAATGATTCGAAAAGAC
  GGCATGCTCTTGTTTAATTTGCATCAGGGCGCCTTATTACAAGGAATGACAGCAACACAG
  TTACTACTACTTGTGTTTTTTAGTTCAACATTTACAGCGTGCTCGGTCACAATGACGATG
  CTTTTGAAACATTTAGGTGGTCAGTCAGCACTAAAATTAATTGGAAAGCAAATGGTGACA
  TCATTGTCTTTAGTTATTGGTGTAGGCATCATTGTTAAAATAGTAATGCTGATTATTTAA
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⊟Protein

⊟General

• locus tag: SAUSA300_2520 [new locus tag: SAUSA300_RS13985 ]
• symbol: SAUSA300_2520
• description: transporter gate domain-containing protein
• length: 439
• theoretical pI: 8.65265
• theoretical MW: 48999.2
• GRAVY: 0.64738

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Cations and iron carrying compounds ferrous iron transport protein B (TIGR00437; HMM-score: 79.4)
• TheSEED  :
  • Ferrous iron transport protein B
  RCJ pfr  Ferrous iron transport protein B
• PFAM:
  no clan defined Gate; Nucleoside recognition (PF07670; HMM-score: 46.4)
  and 3 more
  Phage_holin_3_7; Putative 3TM holin, Phage_holin_3 (PF05449; HMM-score: 16.2)
  Bunya_G2; Bunyavirus glycoprotein G2 (PF03563; HMM-score: 13.8)
  FeoB_C; Ferrous iron transport protein B C terminus (PF07664; HMM-score: 12.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 8
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.003432
  • TAT(Tat/SPI): 0.000176
  • LIPO(Sec/SPII): 0.000335
• predicted transmembrane helices (TMHMM): 8

⊟Accession numbers

• GI: 87160133 NCBI
• RefSeq: YP_495154 NCBI
• UniProt: A0A0H2XDX8 UniProt

⊟Protein sequence

• MRTGHYTPIPNEPHYLVISHADKLTATEKAKLRLLIIKQKLDISLAESVVSSPIASEHVIEQLTLFQHERRHLRPKISATFLAWLLIFLMFALPIGIAYQFSDWFQNQYVSAWIEYLTQTTLLNHDILQHILFGDYGVLSLGTYSLVWALPVVILISLSTAIIDQTGLKSWMIWAIEPSMLWIGLQGNDIVPLLEGFGCNAAAISQAAHQCHTCTKTQCMSLISFGSSCSYQIGATLSIFSVAGKSWLFMPYLILVLLGGILHNRIWLKKNDQQLSVPLPYDRQLHMPNIRQMLLQMWQNIQMFIVQALPIFITICLIVSILSLTPILNVLSQIFTPILSLLGISSELSPGILFSMIRKDGMLLFNLHQGALLQGMTATQLLLLVFFSSTFTACSVTMTMLLKHLGGQSALKLIGKQMVTSLSLVIGVGIIVKIVMLII

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAUSA300_2519 < SAUSA300_2520 < SAUSA300_2521

⊟Regulation

• regulator: Zur* (repression) regulon

  Zur*

  (TF)

  important in Zinc homeostasis; RegPrecise    transcription unit transferred from N315 data RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications