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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1173 [new locus tag: SAUSA300_RS06345 ]
  • pan locus tag?: SAUPAN003582000
  • symbol: SAUSA300_1173
  • pan gene symbol?: —
  • synonym:
  • product: putative acetoacetyl-CoA reductase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_1173 [new locus tag: SAUSA300_RS06345 ]
  • symbol: SAUSA300_1173
  • product: putative acetoacetyl-CoA reductase
  • replicon: chromosome
  • strand: +
  • coordinates: 1291991..1292695
  • length: 705
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGAAAGCATTAGTATTAGGTGGTTCTGGTTCAATTGGTTCTGAGATAGTCAAACAATTA
    TTAACTGATGGATTTGAAGTTTATGTGCAATATTATCGTACTGATATAAATGAATTAACT
    AGCAAATTTAATGATGATAAAGTTCGTTTTATACAAGCGGATTTATCTCAAACAATTGAT
    ATTGACAAAACATTTGGTGACATTAAATCATTAGACTGTTTAATATATGCAAGTGGTCAG
    TCTTTATATGGTGTTTTACAAGATATGAAAGACCATGATATTGATGCATGTTATCAGTTA
    AATGTCTTGCAATTAATTCGATTATGTAGATATTTCGTTGATGTTTTACGTCAAAGTGCC
    AATGGAAGAATTATTGTAATTTCATCAATTTGGGGTGAGACAGGAGCTAGTATGGAAACT
    ATTTATTCGGCGATGAAAAGTGCACAATTAGGTTTCGTTAAGGCGCTTAGTCAAGAGCTT
    GCACTAACATCAGTGACAGTAAATGCTATCGCACCTGGATTTGTAGCCGGTAATATGGCA
    AGTGAGTGGCAAGAAGATGAACTTCAAGCAATGATAACTGAATTACCACAACAGCGATTG
    GTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGT
    GTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    705

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_1173 [new locus tag: SAUSA300_RS06345 ]
  • symbol: SAUSA300_1173
  • description: putative acetoacetyl-CoA reductase
  • length: 234
  • theoretical pI: 4.61995
  • theoretical MW: 25924.4
  • GRAVY: 0.0589744

⊟Function[edit | edit source]

  • TIGRFAM:
    acetoacetyl-CoA reductase (TIGR01829; EC 1.1.1.36; HMM-score: 142.7)
    Metabolism Fatty acid and phospholipid metabolism Biosynthesis 3-oxoacyl-[acyl-carrier-protein] reductase (TIGR01830; EC 1.1.1.100; HMM-score: 140.9)
    and 18 more
    3-hydroxybutyrate dehydrogenase (TIGR01963; HMM-score: 97.6)
    Metabolism Fatty acid and phospholipid metabolism Biosynthesis putative 3-oxoacyl-(acyl-carrier-protein) reductase (TIGR01831; HMM-score: 85.3)
    Metabolism Energy metabolism Fermentation acetoin reductases (TIGR02415; EC 1.1.1.-; HMM-score: 85.1)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides 2-deoxy-D-gluconate 3-dehydrogenase (TIGR01832; EC 1.1.1.125; HMM-score: 81.5)
    Unknown function Enzymes of unknown specificity SDR family mycofactocin-dependent oxidoreductase (TIGR04504; EC 1.1.99.-; HMM-score: 77.4)
    2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase (TIGR04316; EC 1.3.1.28; HMM-score: 73.4)
    Unknown function Enzymes of unknown specificity SDR family mycofactocin-dependent oxidoreductase (TIGR03971; EC 1.1.99.-; HMM-score: 67.7)
    2-hydroxycyclohexanecarboxyl-CoA dehydrogenase (TIGR03206; EC 1.1.1.-; HMM-score: 54)
    pteridine reductase (TIGR02685; EC 1.5.1.33; HMM-score: 47.2)
    cis-2,3-dihydrobiphenyl-2,3-diol dehydrogenase (TIGR03325; EC 1.3.1.56; HMM-score: 46.3)
    rhamnulose-1-phosphate aldolase/alcohol dehydrogenase (TIGR02632; EC 1.1.1.1,4.1.2.19; HMM-score: 38)
    Metabolism Energy metabolism Sugars UDP-glucose 4-epimerase GalE (TIGR01179; EC 5.1.3.2; HMM-score: 27.5)
    sepiapterin reductase (TIGR01500; EC 1.1.1.153; HMM-score: 26.6)
    Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides dTDP-glucose 4,6-dehydratase (TIGR01181; EC 4.2.1.46; HMM-score: 16)
    hopanoid-associated sugar epimerase (TIGR03466; HMM-score: 15.9)
    ergot alkaloid biosynthesis protein, AFUA_2G17970 family (TIGR03649; HMM-score: 14.8)
    UDP-N-acetylglucosamine 4,6-dehydratase (inverting) (TIGR03589; EC 4.2.1.115; HMM-score: 14.3)
    Hypothetical proteins Conserved TIGR01777 family protein (TIGR01777; HMM-score: 13.4)
  • TheSEED  :
    • 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100)
    Fatty Acids, Lipids, and Isoprenoids Fatty acids Fatty Acid Biosynthesis FASII  3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100)
  • PFAM:
    NADP_Rossmann (CL0063) adh_short; short chain dehydrogenase (PF00106; HMM-score: 124)
    adh_short_C2; Enoyl-(Acyl carrier protein) reductase (PF13561; HMM-score: 118)
    and 9 more
    Epimerase; NAD dependent epimerase/dehydratase family (PF01370; HMM-score: 31.1)
    NAD_binding_10; NAD(P)H-binding (PF13460; HMM-score: 30.8)
    KR; KR domain (PF08659; HMM-score: 27.9)
    3Beta_HSD; 3-beta hydroxysteroid dehydrogenase/isomerase family (PF01073; HMM-score: 21.5)
    NmrA; NmrA-like family (PF05368; HMM-score: 21.5)
    GDP_Man_Dehyd; GDP-mannose 4,6 dehydratase (PF16363; HMM-score: 21.5)
    Polysacc_synt_2; Polysaccharide biosynthesis protein (PF02719; HMM-score: 13.6)
    RmlD_sub_bind; RmlD substrate binding domain (PF04321; HMM-score: 12.6)
    HTH (CL0123) RepL; Firmicute plasmid replication protein (RepL) (PF05732; HMM-score: 11.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.007583
    • TAT(Tat/SPI): 0.000124
    • LIPO(Sec/SPII): 0.001252
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKALVLGGSGSIGSEIVKQLLTDGFEVYVQYYRTDINELTSKFNDDKVRFIQADLSQTIDIDKTFGDIKSLDCLIYASGQSLYGVLQDMKDHDIDACYQLNVLQLIRLCRYFVDVLRQSANGRIIVISSIWGETGASMETIYSAMKSAQLGFVKALSQELALTSVTVNAIAPGFVAGNMASEWQEDELQAMITELPQQRLVLPSEVAHTCAYLYHPNARSVTGTIQKVNGAWYI

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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