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⊟Summary[edit | edit source]
- pan ID?: SAUPAN003582000
- symbol?: —
- synonym:
- description?: 3-oxoacyl-ACP reductase
- 3-oxoacyl-ACP reductase
- SDR family NAD(P)-dependent oxidoreductase
- acetoacetyl-CoA reductase
- putative acetoacetyl-CoA reductase
- short chain dehydrogenase, putative
- 3-ketoacyl-
- 3-oxoacyl-[acyl-carrier protein] reductase
- acetoacetyl-CoA reductase, putative
- pseudogene
- putative 3-oxoacyl-acyl carrier protein reductase
- putative short chain dehydrogenase
- short-chain dehydrogenase/reductase SDR
- short chain dehydrogenase family protein
descriptions from strain specific annotations:
- strand?: +
- coordinates?: 3922370..3923075
- synteny block?: BlockID0026590
- occurrence?: in 100% of 34 strains
efpI (ymfI) : 5-aminopentanonyl-elongation factor P reductase [1]
Elongation factor P is required for ribosomes to process mRNA containing tandem proline codons; however, the active site lysine of EF-P requires a post-translational modification. In firmicutes, EF-P is modified with 5-aminopentanol in a pathway that is poorly understood. The only definitive enzyme currently known in the posttranslational modification pathway is EfpI, the final ketoreductase enzyme that reduces 5-amino-3-pentanonyl-EF-P to 5-amino-3-pentanolyl-EF-P.
⊟Orthologs[edit | edit source]
04-02981:
SA2981_1238
08BA02176:
C248_1313
11819-97:
MS7_1238
6850:
RSAU_001163
71193:
ST398NM01_1282
ECT-R 2:
ECTR2_1136
ED133:
SAOV_1282
ED98:
SAAV_1255
HO 5096 0412:
SAEMRSA15_11130
JH1:
SaurJH1_1366
JH9:
SaurJH9_1340
JKD6008:
SAA6008_01235
JKD6159:
SAA6159_01135
JSNZ:
JSNZ_001252
LGA251:
SARLGA251_11910
M013:
M013TW_1222
MRSA252:
SAR1256
MSHR1132:
SAMSHR1132_11230
MSSA476:
SAS1214
Mu3:
SAHV_1270
Mu50:
SAV1280
MW2:
MW1163
RF122:
SAB1142
ST398:
SAPIG1282
T0131:
SAT0131_01336
TCH60:
HMPREF0772_11948 (phbB)
TW20:
SATW20_12740
USA300_TCH1516:
USA300HOU_1212
VC40:
SAVC_05565
⊟Genome Viewer[edit | edit source]
| COL | |
| N315 | |
| NCTC8325 | |
| Newman | |
| USA300_FPR3757 |
⊟Alignments[edit | edit source]
- alignment of orthologues: CLUSTAL format alignment by MAFFT L-INS-i (v7.307)
COL MKALVLGGSGSIGSEIVKQLLTDGFEVYVQYYRTDINELTSKFNDDKVRFIQADLSQTID
N315 MKALVLGGSGSIGSEIVKQLLTDGFEVYVQYYRTDINELTSKFNDDKVRFIQADLSQTID
NCTC8325 MKALVLGGSGSIGSEIVKQLLTDGFEVYVQYYRTDINELTSKFNDDKVRFIQADLSQTID
Newman MKALVLGGSGSIGSEIVKQLLTDGFEVYVQYYRTDINELTSKFNDDKVRFIQADLSQTID
USA300_FPR3757 MKALVLGGSGSIGSEIVKQLLTDGFEVYVQYYRTDINELTSKFNDDKVRFIQADLSQTID
************************************************************
COL IDKTFGDIKSLDCLIYASGQSLYGVLQDMKDHDIDACYQLNVLQLIRLCRYFVDVLRQSA
N315 IDKTFGDIKSLDCLIYASGQSLYGVLQDMKDHDIDACYQLNVLQLIRLCRYFVDVLRQSA
NCTC8325 IDKTFGDIKSLDCLIYASGQSLYGVLQDMKDHDIDACYQLNVLQLIRLCRYFVDVLRQSA
Newman IDKTFGDIKSLDCLIYASGQSLYGVLQDMKDHDIDACYQLNVLQLIRLCRYFVDVLRQSA
USA300_FPR3757 IDKTFGDIKSLDCLIYASGQSLYGVLQDMKDHDIDACYQLNVLQLIRLCRYFVDVLRQSA
************************************************************
COL NGRIIVISSIWGETGASMETIYSAMKSAQLGFVKALSQELALTSVTVNAIAPGFVAGNMA
N315 NGRIIVISSIWGETGASMETIYSAMKSAQLGFVKALSQELALTSVTVNAIAPGFVAGNMA
NCTC8325 NGRIIVISSIWGETGASMETIYSAMKSAQLGFVKALSQELALTSVTVNAIAPGFVAGNMA
Newman NGRIIVISSIWGETGASMETIYSAMKSAQLGFVKALSQELALTSVTVNAIAPGFVAGNMA
USA300_FPR3757 NGRIIVISSIWGETGASMETIYSAMKSAQLGFVKALSQELALTSVTVNAIAPGFVAGNMA
************************************************************
COL SEWQEDELQAMITELPQQRLVLPSEVAHTCAYLYHPNARSVTGTIQKVNGAWYI
N315 SEWQEDELQAMITELPQQRLVLPSEVAHTCAYLYHSNARSVTGTIQKVNGAWYI
NCTC8325 SEWQEDELQAMITELPQQRLVLPSEVAHTCAYLYHPNARSVTGTIQKVNGAWYI
Newman SEWQEDELQAMITELPQQRLVLPSEVAHTCAYLYHPNARSVTGTIQKVNGAWYI
USA300_FPR3757 SEWQEDELQAMITELPQQRLVLPSEVAHTCAYLYHPNARSVTGTIQKVNGAWYI
***********************************.******************
- ↑ Katherine R Hummels, Anne Witzky, Andrei Rajkovic, Rodney Tollerson, Lisa A Jones, Michael Ibba, Daniel B Kearns
Carbonyl reduction by YmfI in Bacillus subtilis prevents accumulation of an inhibitory EF-P modification state.
Mol Microbiol: 2017, 106(2);236-251
[PubMed:28787546] [WorldCat.org] [DOI] (I p)Anne Witzky, Katherine R Hummels, Rodney Tollerson, Andrei Rajkovic, Lisa A Jones, Daniel B Kearns, Michael Ibba
EF-P Posttranslational Modification Has Variable Impact on Polyproline Translation in Bacillus subtilis.
mBio: 2018, 9(2);
[PubMed:29615499] [WorldCat.org] [DOI] (I e)Alexander Golubev, Luc Negroni, Filipp Krasnovid, Shamil Validov, Gulnara Yusupova, Marat Yusupov, Konstantin Usachev
Posttranslational modification of Elongation Factor P from Staphylococcus aureus.
FEBS Open Bio: 2020, 10(7);1342-1347
[PubMed:32436337] [WorldCat.org] [DOI] (I p)Erhard Bremer, Alexandra Calteau, Antoine Danchin, Colin Harwood, John D Helmann, Claudine Médigue, Bernhard O Palsson, Agnieszka Sekowska, David Vallenet, Abril Zuniga, Cristal Zuniga
A model industrial workhorse: Bacillus subtilis strain 168 and its genome after a quarter of a century.
Microb Biotechnol: 2023, 16(6);1203-1231
[PubMed:37002859] [WorldCat.org] [DOI] (I p)