PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

FunGene: 08-OCT-2024

⊟Summary

• organism: Staphylococcus aureus JSNZ
• locus tag: JSNZ_001252
• pan locus tag^?: SAUPAN003582000
• symbol: JSNZ_001252
• pan gene symbol^?: —
• synonym:
• product: SDR family NAD(P)-dependent oxidoreductase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: JSNZ_001252
• symbol: JSNZ_001252
• product: SDR family NAD(P)-dependent oxidoreductase
• replicon: chromosome
• strand: +
• coordinates: 1266617..1267321
• length: 705
• essential: unknown other strains

⊟Accession numbers

• Gene ID:
• RefSeq:
• BioCyc:
• MicrobesOnline:

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  
  ATGAAAGCATTAGTATTAGGTGGTTCTGGTTCAATTGGTTCTGAGATAGTCAAACGATTA
  TTAACTGATGGATTTGAAGTTTATGTGCAATATTATCGTACTGATATAAATGAATTAACT
  AGCAAATTTAATGATGATAAAGTTCGTTTTATACAAGCGGATTTATCTCAAACAATTGAT
  ATTGACAAAACATTTGGTGACATTAAATCATTAGACTGTTTAATATATGCAAGTGGTCAG
  TCTTTATATGGTGTTTTACAAGATATGAAAGACCATGATATTGATGCATGTTATCAGTTA
  AATGTCTTGCAATTAATTCGATTATGTAGATATTTCGTTGATGTTTTACGTCAAAGTGCC
  AATGGAAGAATTATTGTAATTTCATCAATTTGGGGTGAGACAGGAGCTAGTATGGAAACT
  ATTTATTCGGCGATGAAAAGTGCACAATTAGGTTTCGTTAAGGCGCTTAGTCAAGAGCTT
  GCACTAACATCAGTGACAGTAAATGCTATCGCACCTGGATTTGTAGCCGGTAATATGGCA
  AGTGAGTGGCAAGAAGATGAACTTCAAGCAATGATAACTGAATTACCACAACAGCGATTG
  GTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGT
  GTTACTGGAACTATACAGAAAGTTAATGGCGCTTGGTATATTTAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
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  660
  705
  

⊟Protein

⊟General

• locus tag: JSNZ_001252
• symbol: JSNZ_001252
• description: SDR family NAD(P)-dependent oxidoreductase
• length: 234
• theoretical pI: 4.73495
• theoretical MW: 25952.5
• GRAVY: 0.0547009

⊟Function

• TIGRFAM:
  acetoacetyl-CoA reductase (TIGR01829; EC 1.1.1.36; HMM-score: 142.9)
  Metabolism Fatty acid and phospholipid metabolism Biosynthesis 3-oxoacyl-[acyl-carrier-protein] reductase (TIGR01830; EC 1.1.1.100; HMM-score: 140.7)
  and 18 more
  3-hydroxybutyrate dehydrogenase (TIGR01963; HMM-score: 97.7)
  Metabolism Energy metabolism Fermentation acetoin reductases (TIGR02415; EC 1.1.1.-; HMM-score: 87)
  Metabolism Fatty acid and phospholipid metabolism Biosynthesis putative 3-oxoacyl-(acyl-carrier-protein) reductase (TIGR01831; HMM-score: 85.4)
  Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides 2-deoxy-D-gluconate 3-dehydrogenase (TIGR01832; EC 1.1.1.125; HMM-score: 81)
  Unknown function Enzymes of unknown specificity SDR family mycofactocin-dependent oxidoreductase (TIGR04504; EC 1.1.99.-; HMM-score: 77.9)
  2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase (TIGR04316; EC 1.3.1.28; HMM-score: 73.2)
  Unknown function Enzymes of unknown specificity SDR family mycofactocin-dependent oxidoreductase (TIGR03971; EC 1.1.99.-; HMM-score: 67.7)
  2-hydroxycyclohexanecarboxyl-CoA dehydrogenase (TIGR03206; EC 1.1.1.-; HMM-score: 56.5)
  cis-2,3-dihydrobiphenyl-2,3-diol dehydrogenase (TIGR03325; EC 1.3.1.56; HMM-score: 48.8)
  pteridine reductase (TIGR02685; EC 1.5.1.33; HMM-score: 47.8)
  rhamnulose-1-phosphate aldolase/alcohol dehydrogenase (TIGR02632; EC 1.1.1.1,4.1.2.19; HMM-score: 38.8)
  sepiapterin reductase (TIGR01500; EC 1.1.1.153; HMM-score: 26.8)
  Metabolism Energy metabolism Sugars UDP-glucose 4-epimerase GalE (TIGR01179; EC 5.1.3.2; HMM-score: 26.1)
  hopanoid-associated sugar epimerase (TIGR03466; HMM-score: 15.4)
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides dTDP-glucose 4,6-dehydratase (TIGR01181; EC 4.2.1.46; HMM-score: 15.2)
  ergot alkaloid biosynthesis protein, AFUA_2G17970 family (TIGR03649; HMM-score: 14.6)
  UDP-N-acetylglucosamine 4,6-dehydratase (inverting) (TIGR03589; EC 4.2.1.115; HMM-score: 14)
  Hypothetical proteins Conserved TIGR01777 family protein (TIGR01777; HMM-score: 13.3)
• TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
• PFAM:
  NADP_Rossmann (CL0063) adh_short; short chain dehydrogenase (PF00106; HMM-score: 125.3)
  adh_short_C2; Enoyl-(Acyl carrier protein) reductase (PF13561; HMM-score: 117.3)
  and 10 more
  Epimerase; NAD dependent epimerase/dehydratase family (PF01370; HMM-score: 33.8)
  KR; KR domain (PF08659; HMM-score: 31.6)
  NAD_binding_10; NAD(P)H-binding (PF13460; HMM-score: 26.8)
  NmrA; NmrA-like family (PF05368; HMM-score: 23.5)
  GDP_Man_Dehyd; GDP-mannose 4,6 dehydratase (PF16363; HMM-score: 23.2)
  3Beta_HSD; 3-beta hydroxysteroid dehydrogenase/isomerase family (PF01073; HMM-score: 20.8)
  HTH (CL0123) RepL; Firmicute plasmid replication protein (RepL) (PF05732; HMM-score: 12.8)
  Fe_dep_repress; Iron dependent repressor, N-terminal DNA binding domain (PF01325; HMM-score: 12.6)
  NADP_Rossmann (CL0063) Polysacc_synt_2; Polysaccharide biosynthesis protein (PF02719; HMM-score: 12.3)
  RmlD_sub_bind; RmlD substrate binding domain (PF04321; HMM-score: 11.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.67
  • Cytoplasmic Membrane Score: 0.01
  • Cellwall Score: 0.15
  • Extracellular Score: 0.17
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9615
  • Cytoplasmic Membrane Score: 0.0262
  • Cell wall & surface Score: 0.0002
  • Extracellular Score: 0.0121
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.007013
  • TAT(Tat/SPI): 0.000143
  • LIPO(Sec/SPII): 0.001278
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq:
• UniProt:

⊟Protein sequence

• MKALVLGGSGSIGSEIVKRLLTDGFEVYVQYYRTDINELTSKFNDDKVRFIQADLSQTIDIDKTFGDIKSLDCLIYASGQSLYGVLQDMKDHDIDACYQLNVLQLIRLCRYFVDVLRQSANGRIIVISSIWGETGASMETIYSAMKSAQLGFVKALSQELALTSVTVNAIAPGFVAGNMASEWQEDELQAMITELPQQRLVLPSEVAHTCAYLYHPNARSVTGTIQKVNGAWYI

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications