PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_1189 [new locus tag: NWMN_RS06700 ]
• pan locus tag^?: SAUPAN003582000
• symbol: NWMN_1189
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_1189 [new locus tag: NWMN_RS06700 ]
• symbol: NWMN_1189
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 1311977..1312681
• length: 705
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5330706 NCBI
• RefSeq: YP_001332223 NCBI
• BioCyc:
• MicrobesOnline: 3706741 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGAAAGCATTAGTATTAGGTGGTTCTGGTTCAATTGGTTCTGAGATAGTCAAACAATTA
  TTAACTGATGGATTTGAAGTTTATGTGCAATATTATCGTACTGATATAAATGAATTAACT
  AGCAAATTTAATGATGATAAAGTTCGTTTTATACAAGCGGATTTATCTCAAACAATTGAT
  ATTGACAAAACATTTGGTGACATTAAATCATTAGACTGTTTAATATATGCAAGTGGTCAG
  TCTTTATATGGTGTTTTACAAGATATGAAAGACCATGATATTGATGCATGTTATCAGTTA
  AATGTCTTGCAATTAATTCGATTATGTAGATATTTCGTTGATGTTTTACGTCAAAGTGCC
  AATGGAAGAATTATTGTAATTTCATCAATTTGGGGTGAGACAGGAGCTAGTATGGAAACT
  ATTTATTCGGCGATGAAAAGTGCACAATTAGGTTTCGTTAAGGCGCTTAGTCAAGAGCTT
  GCACTAACATCAGTGACAGTAAATGCTATCGCACCTGGATTTGTAGCCGGTAATATGGCA
  AGTGAGTGGCAAGAAGATGAACTTCAAGCAATGATAACTGAATTACCACAACAGCGATTG
  GTTTTACCGAGTGAGGTTGCTCATACATGCGCCTATTTATATCACCCAAATGCTAGAAGT
  GTTACTGGAACTATACAGAAAGTTAATGGTGCTTGGTATATTTAA
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⊟Protein

⊟General

• locus tag: NWMN_1189 [new locus tag: NWMN_RS06700 ]
• symbol: NWMN_1189
• description: hypothetical protein
• length: 234
• theoretical pI: 4.61995
• theoretical MW: 25924.4
• GRAVY: 0.0589744

⊟Function

• TIGRFAM:
  acetoacetyl-CoA reductase (TIGR01829; EC 1.1.1.36; HMM-score: 142.7)
  Metabolism Fatty acid and phospholipid metabolism Biosynthesis 3-oxoacyl-[acyl-carrier-protein] reductase (TIGR01830; EC 1.1.1.100; HMM-score: 140.9)
  and 18 more
  3-hydroxybutyrate dehydrogenase (TIGR01963; HMM-score: 97.6)
  Metabolism Fatty acid and phospholipid metabolism Biosynthesis putative 3-oxoacyl-(acyl-carrier-protein) reductase (TIGR01831; HMM-score: 85.3)
  Metabolism Energy metabolism Fermentation acetoin reductases (TIGR02415; EC 1.1.1.-; HMM-score: 85.1)
  Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides 2-deoxy-D-gluconate 3-dehydrogenase (TIGR01832; EC 1.1.1.125; HMM-score: 81.5)
  Unknown function Enzymes of unknown specificity SDR family mycofactocin-dependent oxidoreductase (TIGR04504; EC 1.1.99.-; HMM-score: 77.4)
  2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase (TIGR04316; EC 1.3.1.28; HMM-score: 73.4)
  Unknown function Enzymes of unknown specificity SDR family mycofactocin-dependent oxidoreductase (TIGR03971; EC 1.1.99.-; HMM-score: 67.7)
  2-hydroxycyclohexanecarboxyl-CoA dehydrogenase (TIGR03206; EC 1.1.1.-; HMM-score: 54)
  pteridine reductase (TIGR02685; EC 1.5.1.33; HMM-score: 47.2)
  cis-2,3-dihydrobiphenyl-2,3-diol dehydrogenase (TIGR03325; EC 1.3.1.56; HMM-score: 46.3)
  rhamnulose-1-phosphate aldolase/alcohol dehydrogenase (TIGR02632; EC 1.1.1.1,4.1.2.19; HMM-score: 38)
  Metabolism Energy metabolism Sugars UDP-glucose 4-epimerase GalE (TIGR01179; EC 5.1.3.2; HMM-score: 27.5)
  sepiapterin reductase (TIGR01500; EC 1.1.1.153; HMM-score: 26.6)
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides dTDP-glucose 4,6-dehydratase (TIGR01181; EC 4.2.1.46; HMM-score: 16)
  hopanoid-associated sugar epimerase (TIGR03466; HMM-score: 15.9)
  ergot alkaloid biosynthesis protein, AFUA_2G17970 family (TIGR03649; HMM-score: 14.8)
  UDP-N-acetylglucosamine 4,6-dehydratase (inverting) (TIGR03589; EC 4.2.1.115; HMM-score: 14.3)
  Hypothetical proteins Conserved TIGR01777 family protein (TIGR01777; HMM-score: 13.4)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  NADP_Rossmann (CL0063) adh_short; short chain dehydrogenase (PF00106; HMM-score: 124)
  adh_short_C2; Enoyl-(Acyl carrier protein) reductase (PF13561; HMM-score: 118)
  and 9 more
  Epimerase; NAD dependent epimerase/dehydratase family (PF01370; HMM-score: 31.1)
  NAD_binding_10; NAD(P)H-binding (PF13460; HMM-score: 30.8)
  KR; KR domain (PF08659; HMM-score: 27.9)
  3Beta_HSD; 3-beta hydroxysteroid dehydrogenase/isomerase family (PF01073; HMM-score: 21.5)
  NmrA; NmrA-like family (PF05368; HMM-score: 21.5)
  GDP_Man_Dehyd; GDP-mannose 4,6 dehydratase (PF16363; HMM-score: 21.5)
  Polysacc_synt_2; Polysaccharide biosynthesis protein (PF02719; HMM-score: 13.6)
  RmlD_sub_bind; RmlD substrate binding domain (PF04321; HMM-score: 12.6)
  HTH (CL0123) RepL; Firmicute plasmid replication protein (RepL) (PF05732; HMM-score: 11.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.67
  • Cytoplasmic Membrane Score: 0.01
  • Cellwall Score: 0.15
  • Extracellular Score: 0.17
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.007583
  • TAT(Tat/SPI): 0.000124
  • LIPO(Sec/SPII): 0.001252
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151221401 NCBI
• RefSeq: YP_001332223 NCBI
• UniProt: A0A0H3KG54 UniProt

⊟Protein sequence

• MKALVLGGSGSIGSEIVKQLLTDGFEVYVQYYRTDINELTSKFNDDKVRFIQADLSQTIDIDKTFGDIKSLDCLIYASGQSLYGVLQDMKDHDIDACYQLNVLQLIRLCRYFVDVLRQSANGRIIVISSIWGETGASMETIYSAMKSAQLGFVKALSQELALTSVTVNAIAPGFVAGNMASEWQEDELQAMITELPQQRLVLPSEVAHTCAYLYHPNARSVTGTIQKVNGAWYI

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: ftsK > NWMN_1186 > NWMN_1187 > NWMN_1188 > NWMN_1189 > NWMN_1190 > NWMN_1191 > pgsA

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications