PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_1170 [new locus tag: SAUSA300_RS06330 ]
• pan locus tag^?: SAUPAN003578000
• symbol: SAUSA300_1170
• pan gene symbol^?: —
• synonym:
• product: GntR family transcriptional regulator

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_1170 [new locus tag: SAUSA300_RS06330 ]
• symbol: SAUSA300_1170
• product: GntR family transcriptional regulator
• replicon: chromosome
• strand: +
• coordinates: 1288696..1289409
• length: 714
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3913264 NCBI
• RefSeq: YP_493867 NCBI
• BioCyc: see SAUSA300_RS06330
• MicrobesOnline: 1292685 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGTCAGAAATGAATGCGGTATATAACGTTAAACAATACATTTTAAATTTGATTAAGCAA
  AATAAATTGGAATATGGTGACCAACTTCCAAGTAATTTATCAATTGCCAGAGAATTAAAT
  GTAAAAACCGACGATGTTTATGAAGCAATTCAGGCATTGATTACTGAACAAGTCATTAAA
  GATAATTTTGAAGAGGGCACAAGTGTTAAGTCACTGCCCCCTTTCTTTTATCCATTGAAT
  GAACTTATAAGTATTGGGCAAATGATTAAAAATGCAGGATTTGAATGCGGAACTGAATAC
  TTAAATTTTGATGAGCAACCAGCAACTATGTTAGATGCAAATTTGTTGAGCGTTGAAGAA
  GGATATCCAGTAACCATTATAGAACGATTACGAACTGCCGATGGAGAACCGGTCGTCTAT
  TGTTTAGATAAAATTGCTAAAAAAGAATTAACATGTACAGAGTATCAAATGAGCAATGGA
  TCGATTCTAAGTGCGATAAAAGAACAAAGTAATCATAATATTTGTTATGCTGATACAGAA
  ATTGAAGCGGTAAATTATGAACCTCGAATATCCGAAGTACTGAATGCTTCGCCACACGAA
  GGTTTGATTTTATTAAAAATTACGCACTATAATGAATCAGATGAACCTATTTTGTATTCA
  TTAAATTATATGAAAAATAGCTTAGTTCAATTTAAAATCACTAGAAAAATATAG
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⊟Protein

⊟General

• locus tag: SAUSA300_1170 [new locus tag: SAUSA300_RS06330 ]
• symbol: SAUSA300_1170
• description: GntR family transcriptional regulator
• length: 237
• theoretical pI: 4.33604
• theoretical MW: 26950.5
• GRAVY: -0.267511

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Anions phosphonate metabolism transcriptional regulator PhnF (TIGR02325; HMM-score: 75.1)
  Signal transduction Regulatory functions DNA interactions phosphonate metabolism transcriptional regulator PhnF (TIGR02325; HMM-score: 75.1)
  Signal transduction Regulatory functions DNA interactions trehalose operon repressor (TIGR02404; HMM-score: 66.6)
  and 3 more
  Signal transduction Regulatory functions DNA interactions phosphonate utilization transcriptional regulator PhnR (TIGR03337; HMM-score: 53)
  Metabolism Energy metabolism Amino acids and amines histidine utilization repressor (TIGR02018; HMM-score: 28.7)
  Signal transduction Regulatory functions DNA interactions histidine utilization repressor (TIGR02018; HMM-score: 28.7)
• TheSEED  :
  • Transcriptional regulator in cluster with Zn-dependent hydrolase
  Bacterial RNA-metabolizing Zn-dependent hydrolases  Transcriptional regulator in cluster with Zn-dependent hydrolase
• PFAM:
  UTRA (CL0122) UTRA; UTRA domain (PF07702; HMM-score: 96)
  and 3 more
  HTH (CL0123) GntR; Bacterial regulatory proteins, gntR family (PF00392; HMM-score: 32.3)
  no clan defined IFT57; Intra-flagellar transport protein 57 (PF10498; HMM-score: 15.3)
  HTH (CL0123) RPA_C; Replication protein A C terminal (PF08784; HMM-score: 13)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.002985
  • TAT(Tat/SPI): 0.00011
  • LIPO(Sec/SPII): 0.000295
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87160196 NCBI
• RefSeq: YP_493867 NCBI
• UniProt: A0A0H2XG09 UniProt

⊟Protein sequence

• MSEMNAVYNVKQYILNLIKQNKLEYGDQLPSNLSIARELNVKTDDVYEAIQALITEQVIKDNFEEGTSVKSLPPFFYPLNELISIGQMIKNAGFECGTEYLNFDEQPATMLDANLLSVEEGYPVTIIERLRTADGEPVVYCLDKIAKKELTCTEYQMSNGSILSAIKEQSNHNICYADTEIEAVNYEPRISEVLNASPHEGLILLKITHYNESDEPILYSLNYMKNSLVQFKITRKI

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: ftsK > SAUSA300_1170 > SAUSA300_1171 > SAUSA300_1172 > SAUSA300_1173 > SAUSA300_1174 > SAUSA300_1175 > pgsA

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications