PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_1172 [new locus tag: SAUSA300_RS06340 ]
• pan locus tag^?: SAUPAN003580000
• symbol: SAUSA300_1172
• pan gene symbol^?: —
• synonym:
• product: M16 family peptidase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_1172 [new locus tag: SAUSA300_RS06340 ]
• symbol: SAUSA300_1172
• product: M16 family peptidase
• replicon: chromosome
• strand: +
• coordinates: 1290705..1291991
• length: 1287
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3913161 NCBI
• RefSeq: YP_493869 NCBI
• BioCyc: see SAUSA300_RS06340
• MicrobesOnline: 1292687 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  ATGAAAGAGCGTTATTATGAATTAATAGACGAAAGAGTATTCGAACAAGAATTAGAAAAT
  GGTTTACGATTATTTATTATCCCCAAACCAGGTTTTCAAAAGACATTTGTCACTTACACT
  ACACAATTTGGTTCATTAGATAATCAATTCAAACCCCTTGGACAAGACCAATTTGTTACT
  GTACCTGATGGAGTTGCTCACTTTTTAGAACATAAATTATTTGAAAAAGAAGAAGAAGAC
  TTATTTACTGCGTTTGCTGAAGATAACGCACAAGCAAATGCGTTTACAAGCTTTGATCGT
  ACAAGCTACTTGTTCAGTGCAACTGATAATATTGAAAACAACATTAAACGTTTACTTACA
  ATGGTTGAAACGCCTTATTTTACAAAAGAAACTGTTGATAAAGAAAAAGGTATTATTGCA
  GAAGAAATAAAAATGTATCAAGAGCAACCTGGATATAAATTAATGTTTGATACATTGCGT
  GCAATGTATCAACAACATCCAATACGTGTTGATATTGCCGGTAGTGTAGAAAGTATATAC
  GATATTACAAAAGATGATTTGTATCTATGTTATGAAACGTTTTATCATCCATCAAATATG
  GTTTTATTTGTTGTTGGCGATGTGGATCCTGAAGCAATATGTCGAATAGTAAAACAACAC
  GAGGATGCTCGTAATAAAGTTAACCAACCCAAAATCGAACGAGGACTTGTTGATGAACCG
  GAGGATGTTAAAGAAGCATTTGTTACTGAATCTATGAAAATTCAATCACCAAGACTAATG
  CTTGGTTTTAAAAATAAACCATTACAAGAAGCGCCTCAAAAATATGTACAACGTGATTTA
  GAAATGTCATTATTCTTTGAGTTAATTTTTGGGGAAGAAACAGATTTTTATCAGAATTTA
  TTAAACGAAGGACTTATCGATGATACATTTGGTTATCAATTTGTACTAGAGCCGACGTAT
  AGTTTTTCAATCGTGACAAGTGCTACTGAAGAACCAGATAAATTAAAAAAATTATTATTA
  GATGAGTTGCGTGATAAAAAAGGCAATTTCCAAGATGCAGAAGCATTTGAACTTTTGAAA
  AAGCAATTTATAGGTGAATTCATATCAAGTTTAAACTCACCTGAATATATTGCTAATCAA
  TATACTAAATTGTATTTTGAAGGTGTTAGCGTGTTTGACATGTTAGACATTGTTGAAAAT
  ATCACGTTAGATAGTATTAACGAAACGTCATCGTTATATTTAAATTTAGATCAGCAAGTC
  GATAGTCGTTTGGAGATTAAAAAGTAA
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⊟Protein

⊟General

• locus tag: SAUSA300_1172 [new locus tag: SAUSA300_RS06340 ]
• symbol: SAUSA300_1172
• description: M16 family peptidase
• length: 428
• theoretical pI: 4.27653
• theoretical MW: 49910
• GRAVY: -0.428738

⊟Function

• reaction:
  EC 3.4.24.56?  ExPASy
  Insulysin Degradation of insulin, glucagon and other polypeptides. No action on proteins
• TIGRFAM:
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other coenzyme PQQ biosynthesis protein PqqF (TIGR02110; EC 3.4.24.-; HMM-score: 40.3)
• TheSEED  :
  • FIG009210: peptidase, M16 family
  CBSS-257314.1.peg.676  FIG009210: peptidase, M16 family
• PFAM:
  Peptidase_ME (CL0094) Peptidase_M16_C; Peptidase M16 inactive domain (PF05193; HMM-score: 89.2)
  and 1 more
  Peptidase_M16; Insulinase (Peptidase family M16) (PF00675; HMM-score: 55.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.003678
  • TAT(Tat/SPI): 0.000279
  • LIPO(Sec/SPII): 0.000317
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87161659 NCBI
• RefSeq: YP_493869 NCBI
• UniProt: A0A0H2XHJ8 UniProt

⊟Protein sequence

• MKERYYELIDERVFEQELENGLRLFIIPKPGFQKTFVTYTTQFGSLDNQFKPLGQDQFVTVPDGVAHFLEHKLFEKEEEDLFTAFAEDNAQANAFTSFDRTSYLFSATDNIENNIKRLLTMVETPYFTKETVDKEKGIIAEEIKMYQEQPGYKLMFDTLRAMYQQHPIRVDIAGSVESIYDITKDDLYLCYETFYHPSNMVLFVVGDVDPEAICRIVKQHEDARNKVNQPKIERGLVDEPEDVKEAFVTESMKIQSPRLMLGFKNKPLQEAPQKYVQRDLEMSLFFELIFGEETDFYQNLLNEGLIDDTFGYQFVLEPTYSFSIVTSATEEPDKLKKLLLDELRDKKGNFQDAEAFELLKKQFIGEFISSLNSPEYIANQYTKLYFEGVSVFDMLDIVENITLDSINETSSLYLNLDQQVDSRLEIKK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: ftsK > SAUSA300_1170 > SAUSA300_1171 > SAUSA300_1172 > SAUSA300_1173 > SAUSA300_1174 > SAUSA300_1175 > pgsA

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications