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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_RS06340 [old locus tag: SAUSA300_1172 ]
  • pan locus tag?: SAUPAN003580000
  • symbol: SAUSA300_RS06340
  • pan gene symbol?: —
  • synonym:
  • product: peptidase M16

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_RS06340 [old locus tag: SAUSA300_1172 ]
  • symbol: SAUSA300_RS06340
  • product: peptidase M16
  • replicon: chromosome
  • strand: +
  • coordinates: 1290705..1291991
  • length: 1287
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGAAAGAGCGTTATTATGAATTAATAGACGAAAGAGTATTCGAACAAGAATTAGAAAAT
    GGTTTACGATTATTTATTATCCCCAAACCAGGTTTTCAAAAGACATTTGTCACTTACACT
    ACACAATTTGGTTCATTAGATAATCAATTCAAACCCCTTGGACAAGACCAATTTGTTACT
    GTACCTGATGGAGTTGCTCACTTTTTAGAACATAAATTATTTGAAAAAGAAGAAGAAGAC
    TTATTTACTGCGTTTGCTGAAGATAACGCACAAGCAAATGCGTTTACAAGCTTTGATCGT
    ACAAGCTACTTGTTCAGTGCAACTGATAATATTGAAAACAACATTAAACGTTTACTTACA
    ATGGTTGAAACGCCTTATTTTACAAAAGAAACTGTTGATAAAGAAAAAGGTATTATTGCA
    GAAGAAATAAAAATGTATCAAGAGCAACCTGGATATAAATTAATGTTTGATACATTGCGT
    GCAATGTATCAACAACATCCAATACGTGTTGATATTGCCGGTAGTGTAGAAAGTATATAC
    GATATTACAAAAGATGATTTGTATCTATGTTATGAAACGTTTTATCATCCATCAAATATG
    GTTTTATTTGTTGTTGGCGATGTGGATCCTGAAGCAATATGTCGAATAGTAAAACAACAC
    GAGGATGCTCGTAATAAAGTTAACCAACCCAAAATCGAACGAGGACTTGTTGATGAACCG
    GAGGATGTTAAAGAAGCATTTGTTACTGAATCTATGAAAATTCAATCACCAAGACTAATG
    CTTGGTTTTAAAAATAAACCATTACAAGAAGCGCCTCAAAAATATGTACAACGTGATTTA
    GAAATGTCATTATTCTTTGAGTTAATTTTTGGGGAAGAAACAGATTTTTATCAGAATTTA
    TTAAACGAAGGACTTATCGATGATACATTTGGTTATCAATTTGTACTAGAGCCGACGTAT
    AGTTTTTCAATCGTGACAAGTGCTACTGAAGAACCAGATAAATTAAAAAAATTATTATTA
    GATGAGTTGCGTGATAAAAAAGGCAATTTCCAAGATGCAGAAGCATTTGAACTTTTGAAA
    AAGCAATTTATAGGTGAATTCATATCAAGTTTAAACTCACCTGAATATATTGCTAATCAA
    TATACTAAATTGTATTTTGAAGGTGTTAGCGTGTTTGACATGTTAGACATTGTTGAAAAT
    ATCACGTTAGATAGTATTAACGAAACGTCATCGTTATATTTAAATTTAGATCAGCAAGTC
    GATAGTCGTTTGGAGATTAAAAAGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1287

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_RS06340 [old locus tag: SAUSA300_1172 ]
  • symbol: SAUSA300_RS06340
  • description: peptidase M16
  • length: 428
  • theoretical pI: 4.27653
  • theoretical MW: 49910
  • GRAVY: -0.428738

⊟Function[edit | edit source]

  • reaction:
    EC 3.4.24.56?  ExPASy
    Insulysin Degradation of insulin, glucagon and other polypeptides. No action on proteins
  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other coenzyme PQQ biosynthesis protein PqqF (TIGR02110; EC 3.4.24.-; HMM-score: 40.3)
  • TheSEED: see SAUSA300_1172
  • PFAM:
    Peptidase_ME (CL0094) Peptidase_M16_C; Peptidase M16 inactive domain (PF05193; HMM-score: 89.2)
    and 1 more
    Peptidase_M16; Insulinase (Peptidase family M16) (PF00675; HMM-score: 55.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003678
    • TAT(Tat/SPI): 0.000279
    • LIPO(Sec/SPII): 0.000317
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKERYYELIDERVFEQELENGLRLFIIPKPGFQKTFVTYTTQFGSLDNQFKPLGQDQFVTVPDGVAHFLEHKLFEKEEEDLFTAFAEDNAQANAFTSFDRTSYLFSATDNIENNIKRLLTMVETPYFTKETVDKEKGIIAEEIKMYQEQPGYKLMFDTLRAMYQQHPIRVDIAGSVESIYDITKDDLYLCYETFYHPSNMVLFVVGDVDPEAICRIVKQHEDARNKVNQPKIERGLVDEPEDVKEAFVTESMKIQSPRLMLGFKNKPLQEAPQKYVQRDLEMSLFFELIFGEETDFYQNLLNEGLIDDTFGYQFVLEPTYSFSIVTSATEEPDKLKKLLLDELRDKKGNFQDAEAFELLKKQFIGEFISSLNSPEYIANQYTKLYFEGVSVFDMLDIVENITLDSINETSSLYLNLDQQVDSRLEIKK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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