From AureoWiki
Jump to navigation Jump to search

NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1164 [new locus tag: SAUSA300_RS06300 ]
  • pan locus tag?: SAUPAN003572000
  • symbol: truB
  • pan gene symbol?: truB
  • synonym:
  • product: tRNA pseudouridine synthase B

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_1164 [new locus tag: SAUSA300_RS06300 ]
  • symbol: truB
  • product: tRNA pseudouridine synthase B
  • replicon: chromosome
  • strand: +
  • coordinates: 1279404..1280321
  • length: 918
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGTATAATGGGATATTACCAGTATATAAAGAGCGCGGTTTAACAAGTCATGACGTTGTA
    TTCAAATTGCGTAAAATATTAAAAACTAAAAAAATAGGTCACACGGGTACGCTTGATCCC
    GAAGTTGCAGGCGTGTTACCGGTATGTATAGGTAATGCAACGAGAGTTAGTGATTATGTT
    ATGGATATGGGCAAAGCTTATGAAGCAACTGTATCGATAGGAAGAAGTACAACGACTGAA
    GATCAAACGGGTGATACATTGGAAACAAAAGGTGTACACTCAGCAGATTTTAATAAGGAC
    GATATTGACCGATTGTTAGAAAGTTTTAAAGGTATCATTGAACAAATTCCGCCGATGTAC
    TCATCCGTCAAAGTAAATGGTAAAAAATTATATGAATATGCGCGTAATAATGAAACAGTT
    GAAAGACCAAAGCGTAAAGTTAATATTAAAGACATTGGGCGTATATCTGAATTAGATTTT
    AAAGAAAATGAGTGTCATTTTAAAATACGCGTCATCTGTGGTAAAGGTACATATATTAGA
    ACGCTAGCAACTGATATTGGTGTGAAATTAGGCTTTCCGGCACATATGTCGAAATTAACA
    CGAATCGAGTCTGGTGGATTTGTGTTGAAAGATAGCCTTACATTAGAACAAATAAAAGAA
    CTTCATGAGCAGGATTCATTGCAAAATAAATTGTTTCCTTTAGAATATGGATTAAAGGGT
    TTGCCAAGCATTAAAATTAAAGATTCGCACATAAAAAAACGTATTTTAAATGGGCAGAAA
    TTTAATAAAAATGAATTTGATAACAAAATTAAAGACCAAATTGTATTTATTGATGATGAT
    TCAGAAAAAGTATTAGCAATTTATATGGTACACCCTACAAAAGAATCAGAAATTAAACCT
    AAAAAAGTCTTTAATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    918

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_1164 [new locus tag: SAUSA300_RS06300 ]
  • symbol: TruB
  • description: tRNA pseudouridine synthase B
  • length: 305
  • theoretical pI: 9.56269
  • theoretical MW: 34592.7
  • GRAVY: -0.48918

⊟Function[edit | edit source]

  • reaction:
    EC 4.2.1.70?  ExPASy
    Pseudouridylate synthase Uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O
    EC 5.4.99.25?  ExPASy
    tRNA pseudouridine55 synthase tRNA uridine55 = tRNA pseudouridine55
  • TIGRFAM:
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA pseudouridine(55) synthase (TIGR00431; EC 5.4.99.25; HMM-score: 249)
    and 2 more
    Genetic information processing Protein synthesis tRNA and rRNA base modification putative rRNA pseudouridine synthase (TIGR00425; EC 5.4.99.-; HMM-score: 134.3)
    Metabolism Transport and binding proteins Cations and iron carrying compounds tonB-system energizer ExbB (TIGR02805; HMM-score: 11.8)
  • TheSEED  :
    • tRNA pseudouridine synthase B (EC 4.2.1.70)
    RNA Metabolism RNA processing and modification tRNA processing  tRNA pseudouridine synthase B (EC 4.2.1.70)
  • PFAM:
    PseudoU_synth (CL0649) TruB_N; TruB family pseudouridylate synthase (N terminal domain) (PF01509; HMM-score: 183.2)
    and 1 more
    TruB_C_2; tRNA pseudouridylate synthase B C-terminal domain (PF16198; HMM-score: 47)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.007617
    • TAT(Tat/SPI): 0.000587
    • LIPO(Sec/SPII): 0.000837
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MYNGILPVYKERGLTSHDVVFKLRKILKTKKIGHTGTLDPEVAGVLPVCIGNATRVSDYVMDMGKAYEATVSIGRSTTTEDQTGDTLETKGVHSADFNKDDIDRLLESFKGIIEQIPPMYSSVKVNGKKLYEYARNNETVERPKRKVNIKDIGRISELDFKENECHFKIRVICGKGTYIRTLATDIGVKLGFPAHMSKLTRIESGGFVLKDSLTLEQIKELHEQDSLQNKLFPLEYGLKGLPSIKIKDSHIKKRILNGQKFNKNEFDNKIKDQIVFIDDDSEKVLAIYMVHPTKESEIKPKKVFN

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:
    SAUSA300_2142(asp23)alkaline shock protein 23  [1] (data from MRSA252)
    SAUSA300_1096(carB)carbamoyl phosphate synthase large subunit  [1] (data from MRSA252)
    SAUSA300_0965(folD)bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase  [1] (data from MRSA252)
    SAUSA300_1080(ftsZ)cell division protein FtsZ  [1] (data from MRSA252)
    SAUSA300_0532(fusA)elongation factor G  [1] (data from MRSA252)
    SAUSA300_1845(hemL)glutamate-1-semialdehyde aminotransferase  [1] (data from MRSA252)
    SAUSA300_1640(icd)isocitrate dehydrogenase  [1] (data from MRSA252)
    SAUSA300_1330(ilvA)threonine dehydratase  [1] (data from MRSA252)
    SAUSA300_0996(lpdA)dihydrolipoamide dehydrogenase  [1] (data from MRSA252)
    SAUSA300_2541(mqo)malate:quinone oxidoreductase  [1] (data from MRSA252)
    SAUSA300_2055(murA)UDP-N-acetylglucosamine 1-carboxyvinyltransferase  [1] (data from MRSA252)
    SAUSA300_2078(murA)UDP-N-acetylglucosamine 1-carboxyvinyltransferase  [1] (data from MRSA252)
    SAUSA300_0993(pdhA)pyruvate dehydrogenase E1 component, alpha subunit  [1] (data from MRSA252)
    SAUSA300_2089(pdp)pyrimidine-nucleoside phosphorylase  [1] (data from MRSA252)
    SAUSA300_0220(pflB)formate acetyltransferase  [1] (data from MRSA252)
    SAUSA300_1644(pyk)pyruvate kinase  [1] (data from MRSA252)
    SAUSA300_0523(rplA)50S ribosomal protein L1  [1] (data from MRSA252)
    SAUSA300_0525(rplL)50S ribosomal protein L7/L12  [1] (data from MRSA252)
    SAUSA300_2187(rpsE)30S ribosomal protein S5  [1] (data from MRSA252)
    SAUSA300_0562(thiD)phosphomethylpyrimidine kinase  [1] (data from MRSA252)
    SAUSA300_0533(tuf)elongation factor Tu  [1] (data from MRSA252)
    SAUSA300_0364(ychF)GTP-dependent nucleic acid-binding protein EngD  [1] (data from MRSA252)
    SAUSA300_0716ribonucleotide-diphosphate reductase subunit alpha  [1] (data from MRSA252)
    SAUSA300_0844hypothetical protein  [1] (data from MRSA252)
    SAUSA300_0871hypothetical protein  [1] (data from MRSA252)
    SAUSA300_0912enoyl-(acyl carrier protein) reductase  [1] (data from MRSA252)
    SAUSA300_1656universal stress protein  [1] (data from MRSA252)
    SAUSA300_2484hydroxymethylglutaryl-CoA synthase  [1] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 1.19 1.20 1.21 1.22 1.23 1.24 1.25 1.26 1.27 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=SAUSA300_1164&oldid=100452"