PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

⊟Summary

• pan ID^?: SAUPAN001133000
• symbol^?: tarI
• synonym:
• description^?: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

  descriptions from strain specific annotations:

  • 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
  • D-ribitol-5-phosphate cytidylyltransferase
  • 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, putative
  • 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (4-diphosphocytidyl-2C-methyl-D-erythritol synthase) (MEPcytidylyltransferase) (MCT)
  • 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase 2, IspD_2
  • 4-diphosphocytidyl-2C-methyl-D-erythritol synthase, putative
  • ribitol-5-phosphate cytidylyltransferase
• strand^?: +
• coordinates^?: 1402793..1403509
• synteny block^?: BlockID0006530
• occurrence^?: in 100% of 34 strains

tarI1 : ribitol-5-phosphate cytidylyltransferase 1

• A crystal structure is available : 6XHP

• Wall teichoic acid biosynthesis in Staphylococcus aureus is complicated by the presence of multiple overlapping pathways that are at least situationally functionally redundant. Initial steps involve modification of undecaprenyl phosphate with GlcNAc (TarO) and ManNAc (TarA) and then priming with two equivalents of glycerophosphate (TarB, TarF). This makes a starter unit for two types of WTA:
  • TarL can prime and polymerize approximately 40 units of ribitol phosphate resulting into extended-length teichoic acids. The CDP-ribitol substrate is generated by the TarI1 and TarJ1 enzymes
  • TarK can prime and polymerize approximately 20 units of ribitol phosphate resulting into short-length teichoic acids. The CDP-ribitol substrate is generated by the TarI2 and TarJ2 enzymes
    • TarJ encodes ribulose-5-phosphate reductase (alt. ribitol-5-phosphate dehydrogenase) which reduces ribulose phosphate to ribitol phosphate
    • TarI encodes ribitol-5-phosphate cytidylyltransferase which activates ribitol as CDP-ribitol

⊟Orthologs

COL:
SACOL0240 (ispD)
N315:
SA0245 (ispD)
NCTC8325:
SAOUHSC_00225 (ispD)
Newman:
NWMN_0189 (ispD)
USA300_FPR3757:
SAUSA300_0249 (ispD)
04-02981:
SA2981_0255
08BA02176:
C248_0243 (ispD)
11819-97:
MS7_0245 (ispD2)
6850:
RSAU_000199
71193:
ST398NM01_0268
ECT-R 2:
ECTR2_216
ED133:
SAOV_0194
ED98:
SAAV_0223 (ispD_1)
HO 5096 0412:
SAEMRSA15_02140
JH1:
SaurJH1_0246 (ispD)
JH9:
SaurJH9_0240 (ispD)
JKD6008:
SAA6008_00229 (ispD_2)
JKD6159:
SAA6159_00232 (ispD_2)
JSNZ:
JSNZ_000195
LGA251:
SARLGA251_02190 (ispD2)
M013:
M013TW_0236
MRSA252:
SAR0252 (ispD)
MSHR1132:
SAMSHR1132_02230
MSSA476:
SAS0232 (ispD)
Mu3:
SAHV_0254 (ispD)
Mu50:
SAV0255 (ispD)
MW2:
MW0231 (ispD)
RF122:
SAB0194 (ispD)
ST398:
SAPIG0268
T0131:
SAT0131_00248
TCH60:
HMPREF0772_10247 (ispD)
TW20:
SATW20_02570 (ispD2)
USA300_TCH1516:
USA300HOU_0266 (ispD)
VC40:
SAVC_01015 (ispD)

⊟Genome Viewer

COL
N315
NCTC8325
Newman
USA300_FPR3757

⊟Alignments

• alignment of orthologues:
  CLUSTAL format alignment by MAFFT L-INS-i (v7.307)
  
  
  COL             MKYAGILAGGIGSRMGNVPLPKQFLDLDNKPILIHTLEKFILINDFEKIIIATPQQWMTH
  N315            MKYAGILAGGIGSRMGNVPLPKQFLDLDNKPILIHTLEKFILINDFEKIIIATPQQWMTH
  NCTC8325        MKYAGILAGGIGSRMGNVPLPKQFLDLDNKPILIHTLEKFILINDFEKIIIATPQQWMTH
  Newman          MKYAGILAGGIGSRMGNVPLPKQFLDLDNKPILIHTLEKFILINDFEKIIIATPQQWMTH
  USA300_FPR3757  MKYAGILAGGIGSRMGNVPLPKQFLDLDNKPILIHTLEKFILINDFEKIIIATPQQWMTH
                  ************************************************************
  
  COL             TKDTLRKFKISDERIEVIQGGSDRNDTIMNIVKHIESTNGINDDDVIVTHDAVRPFLTHR
  N315            TKDTLRKFKISDERIEVIQGGSDRNDTIMNIVKHIESTNGINDDDVIVTHDAVRPFLTHR
  NCTC8325        TKDTLRKFKISDERIEVIQGGSDRNDTIMNIVKHIESTNGINDDDVIVTHDAVRPFLTHR
  Newman          TKDTLRKFKISDERIEVIQGGSDRNDTIMNIVKHIESTNGINDDDVIVTHDAVRPFLTHR
  USA300_FPR3757  TKDTLRKFKISDERIEVIQGGSDRNDTIMNIVKHIESTNGINDDDVIVTHDAVRPFLTHR
                  ************************************************************
  
  COL             IIKENIQAALEYGAVDTVIDAIDTIVTSKDNQTIDAIPVRNEMYQGQTPQSFNINLLKES
  N315            IIKENIQAALEYGAVDTVIDAIDTIVTSKDNQTIDAIPVRNEMYQGQTPQSFNINLLKES
  NCTC8325        IIKENIQAALEYGAVDTVIDAIDTIVTSKDNQTIDAIPVRNEMYQGQTPQSFNINLLKES
  Newman          IIKENIQAALEYGAVDTVIDAIDTIVTSKDNQTIDAIPVRNEMYQGQTPQSFNINLLKES
  USA300_FPR3757  IIKENIQAALEYGAVDTVIDAIDTIVTSKDNQTIDAIPVRNEMYQGQTPQSFNINLLKES
                  ************************************************************
  
  COL             YAQLSDEQKSILSDACKIIVETNKPVRLVKGELYNIKVTTPYDLKVANAIIRGGIADD
  N315            YAQLSDEQKSILSDACKIIVETNKPVRLVKGELYNIKVTTPYDLKVANAIIRGGIADD
  NCTC8325        YAQLSDEQKSILSDACKIIVETNKPVRLVKGELYNIKVTTPYDLKVANAIIRGGIADD
  Newman          YAQLSDEQKSILSDACKIIVETNKPVRLVKGELYNIKVTTPYDLKVANAIIRGGIADD
  USA300_FPR3757  YAQLSDEQKSILSDACKIIVETNKPVRLVKGELYNIKVTTPYDLKVANAIIRGGIADD
                  **********************************************************