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⊟Summary[edit | edit source]
- pan ID?: SAUPAN001126000
- symbol?: tarL'
- synonym:
- description?: tagB protein
- tagB protein
- CDP-glycerol glycerophosphotransferase family protein
- putative teichoic acid biosynthesis protein B
- teichoic acid biosynthesis protein
- teichoic acid biosynthesis protein, putative
- teichoic acid biosynthesis protein TagB
- teichoic acid ribitol-phosphate polymerase TarK
- glycosyl/glycerophosphate transferase
- putative CDP-Glycerol:Poly(glycerophosphate) glycerophosphotransferase
- Putative polyribitolphosphotransferase
- CDP-glycerol:poly(glycerophosphate) glycerophosphotransferase
- CDP-Glycerol:Poly(glycerophosphate) glycerophosphotransferase family protein
- CDP-ribitol ribitolphosphotransferase
- teichoic acid biosynthesis protein B
descriptions from strain specific annotations:
- strand?: +
- coordinates?: 1398379..1400073
- synteny block?: BlockID0006460
- occurrence?: in 100% of 34 strains
tarK (tarL') : short-length ribitol-phosphate polymerase [1]
• A crystal structure is available : 8V34
- Wall teichoic acid biosynthesis in Staphylococcus aureus is complicated by the presence of multiple overlapping pathways that are at least situationally functionally redundant. Initial steps involve modification of undecaprenyl phosphate with GlcNAc (TarO) and ManNAc (TarA) and then priming with two equivalents of glycerophosphate (TarB, TarF). This makes a starter unit for two types of WTA:
- TarL can prime and polymerize approximately 40 units of ribitol phosphate resulting into extended-length teichoic acids. The CDP-ribitol substrate is generated by the TarI1 and TarJ1 enzymes
- TarK can prime and polymerize approximately 20 units of ribitol phosphate resulting into short-length teichoic acids. The CDP-ribitol substrate is generated by the TarI2 and TarJ2 enzymes
- TarJ encodes ribulose-5-phosphate reductase (alt. ribitol-5-phosphate dehydrogenase) which reduces ribulose phosphate to ribitol phosphate
- TarI encodes ribitol-5-phosphate cytidylyltransferase which activates ribitol as CDP-ribitol
⊟Orthologs[edit | edit source]
04-02981:
SA2981_0253
08BA02176:
C248_0240
11819-97:
MS7_0241
6850:
RSAU_000197
71193:
ST398NM01_0265
ECT-R 2:
ECTR2_214
ED133:
SAOV_0192
ED98:
SAAV_0221
HO 5096 0412:
SAEMRSA15_02120
JH1:
SaurJH1_0244
JH9:
SaurJH9_0238
JKD6008:
SAA6008_00226
JKD6159:
SAA6159_00230
JSNZ:
JSNZ_000193 (tarK)
LGA251:
SARLGA251_02170 (tarK)
M013:
M013TW_0234
MRSA252:
SAR0248
MSHR1132:
SAMSHR1132_02210
MSSA476:
SAS0229
Mu3:
SAHV_0252
Mu50:
SAV0253
MW2:
MW0229
RF122:
SAB0192
ST398:
SAPIG0265
T0131:
SAT0131_00245 (accB)
TCH60:
HMPREF0772_10250
TW20:
SATW20_02550 (tarK)
USA300_TCH1516:
USA300HOU_0264 (tagB1)
VC40:
SAVC_01005
⊟Genome Viewer[edit | edit source]
| COL | |
| N315 | |
| NCTC8325 | |
| Newman | |
| USA300_FPR3757 |
⊟Alignments[edit | edit source]
- alignment of orthologues: CLUSTAL format alignment by MAFFT L-INS-i (v7.307)
COL MTKTKQAIHIDNIYWERVQLYIEGHSEGVDLTSGQFVLRNLTETKTLEANEMKIDGNTFI
N315 MTKTKQAIHIDNIYWERVQLYIEGHSEGVDLTSGQFVLRNLTETKTLEANEMKIDGNTFI
NCTC8325 ---------------------------------------------------MKIDGNTFI
Newman MTKTKQAIHIDNIYWERVQLYIEGHSEGVDLTSGQFVLRNLTETKTLEANEMKIDGNTFI
USA300_FPR3757 MTKTKQAIHIDNIYWERVQLYIEGHSEGVDLTSGQFVLRNLTETKTLEANEMKIDGNTFI
*********
COL CRFNVAILDDGYYLPMDKYLFVYHDQLEYIGQLNPNIIDQAYAALNEEQIEEYNELTTQN
N315 CRFNVAILDDGYYLPMDKYLFVYHDQLEYIGQLNPNIIDQAYAALNEEQIEEYNELTTQN
NCTC8325 CRFNVAILDDGYYLPMDKYLFVYHDQLEYIGQLNPNIIDQAYAALNEEQIEEYNELTTQN
Newman CRFNVAILDDGYYLPMDKYLFVYHDQLEYIGQLNPNIIDQAYAALNEEQIEEYNELTTQN
USA300_FPR3757 CRFNVAILDDGYYLPMDKYLFVYHDQLEYIGQLNPNIIDQAYAALNEEQIEEYNELTTQN
************************************************************
COL GKVNYLLAYDAKVFRKGGVSQHTVYTITPEIASDVNEFVFDIEITLPQEKSGVIATSAHW
N315 GKVNYLLAYDAKVFRKGGVSQHTVYTITPEIASDVNEFVFDIEITLPQEKSGVIATSAHW
NCTC8325 GKVNYLLAYDAKVFRKGGVSQHTVYTITPEIASDVNEFVFDIEITLPQEKSGVIATSAHW
Newman GKVNYLLAYDAKVFRKGGVSQHTVYTITPEIASDVNEFVFDIEITLPQEKSGVIATSAHW
USA300_FPR3757 GKVNYLLAYDAKVFRKGGVSQHTVYTITPEIASDVNEFVFDIEITLPQEKSGVIATSAHW
************************************************************
COL LHKQGHKASFESRSFLFKAIFNITKLLHIKRSKTILFTSDSRPNLSGNFKYVYDELLRQK
N315 LHKQGHKASFESRSFLFKAIFNITKLLHIKRSKTILFTSDSRPNLSGNFKYVYDELLRQK
NCTC8325 LHKQGHKASFESRSFLFKAIFNITKLLHIKRSKTILFTSDSRPNLSGNFKYVYDELLRQK
Newman LHKQGHKASFESRSFLFKAIFNITKLLHIKRSKTILFTSDSRPNLSGNFKYVYDELLRQK
USA300_FPR3757 LHKQGHKASFESRSFLFKAIFNITKLLHIKRSKTILFTSDSRPNLSGNFKYVYDELLRQK
************************************************************
COL VDFDYDIKTVFKENITDRRKWRDKFRLPYLLGKADYIFVDDFHPLIYTVRFRPSQEIIQV
N315 VDFDYDIKTVFKANITDRRKWRDKFRLPYLLGKADYIFVDDFHPLIYTVRFRPSQEIIQV
NCTC8325 VDFDYDIKTVFKENITDRRKWRDKFRLPYLLGKADYIFVDDFHPLIYTVRFRPSQEIIQV
Newman VDFDYDIKTVFKENITDRRKWRDKFRLPYLLGKADYIFVDDFHPLIYTVRFRPSQEIIQV
USA300_FPR3757 VDFDYDIKTVFKENITDRRKWRDKFRLPYLLGKADYIFVDDFHPLIYTVRFRPSQEIIQV
************ ***********************************************
COL WHAVGAFKTVGFSRTGKKGGPFIDSLNHRSYTKAYVSSETDIPFYAEAFGIREENVVPTG
N315 WHAVGAFKTVGFSRTGKKGGPFIDSLNHRSYTKAYVSSETDIPFYAEAFGIREENVVPTG
NCTC8325 WHAVGAFKTVGFSRTGKKGGPFIDSLNHRSYTKAYVSSETDIPFYAEAFGIREENVVPTG
Newman WHAVGAFKTVGFSRTGKKGGPFIDSLNHRSYTKAYVSSETDIPFYAEAFGIREENVVPTG
USA300_FPR3757 WHAVGAFKTVGFSRTGKKGGPFIDSLNHRSYTKAYVSSETDIPFYAEAFGIREENVVPTG
************************************************************
COL VPRTDVLFDEAYATQIKQEMEDELPIIKGKKVILFAPTFRGNGHGTAHYPFFKIDFERLA
N315 VPRTDVLFDEAYATQIKQEMEDELPIIKGKKVILFAPTFRGNGHGTAHYPFFKIDFERLA
NCTC8325 VPRTDVLFDEAYATQIKQEMEDELPIIKGKKVILFAPTFRGNGHGTAHYPFFKIDFERLA
Newman VPRTDVLFDEAYATQIKQEMEDELPIIKGKKVILFAPTFRGNGHGTAHYPFFKIDFERLA
USA300_FPR3757 VPRTDVLFDEAYATQIKQEMEDELPIIKGKKVILFAPTFRGNGHGTAHYPFFKIDFERLA
************************************************************
COL RYCEKHNAVVLFKMHPFVKNRLNISREHRQYFIDVSDHREVNDILFVTDLLISDYSSLIY
N315 RYCEKHNAVVLFKMHPFVKNRLNISREHRQYFIDVSDHREVNDILFVTDLLISDYSSLIY
NCTC8325 RYCEKHNAVVLFKMHPFVKNRLNISREHRQYFIDVSDHREVNDILFVTDLLISDYSSLIY
Newman RYCEKHNAVVLFKMHPFVKNRLNISREHRQYFIDVSDHREVNDILFVTDLLISDYSSLIY
USA300_FPR3757 RYCEKHNAVVLFKMHPFVKNRLNISREHRQYFIDVSDHREVNDILFVTDLLISDYSSLIY
************************************************************
COL EYAVFKKPMIFYAFDLEDYITTRDFYEPFESFVPGKIVQSFDALMDALDNEDYEVEKVVP
N315 EYAVFKKPMIFYAFDLEDYITTRDFYEPYESFVPGKIVQSFDALMDALDTEDYEVEKVVP
NCTC8325 EYAVFKKPMIFYAFDLEDYITTRDFYEPFESFVPGKIVQSFDALMDALDNEDYEVEKVVP
Newman EYAVFKKPMIFYAFDLEDYITTRDFYEPFESFVPGKIVQSFDALMDALDNEDYEVEKVVP
USA300_FPR3757 EYAVFKKPMIFYAFDLEDYITTRDFYEPFESFVPGKIVQSFDALMDALDNEDYEVEKVVP
****************************:********************.**********
COL FLDKHFKYQDGRSSERLVKDLFRR
N315 FLDKHFKYQDGRSSERLVKDLFRR
NCTC8325 FLDKHFKYQDGRSSERLVKDLFRR
Newman FLDKHFKYQDGRSSERLVKDLFRR
USA300_FPR3757 FLDKHFKYQDGRSSERLVKDLFRR
************************
- ↑ Stephanie Brown, Yu-Hui Zhang, Suzanne Walker
A revised pathway proposed for Staphylococcus aureus wall teichoic acid biosynthesis based on in vitro reconstitution of the intracellular steps.
Chem Biol: 2008, 15(1);12-21
[PubMed:18215769] [WorldCat.org] [DOI] (P p)Timothy C Meredith, Jonathan G Swoboda, Suzanne Walker
Late-stage polyribitol phosphate wall teichoic acid biosynthesis in Staphylococcus aureus.
J Bacteriol: 2008, 190(8);3046-56
[PubMed:18281399] [WorldCat.org] [DOI] (I p)Jonathan G Swoboda, Jennifer Campbell, Timothy C Meredith, Suzanne Walker
Wall teichoic acid function, biosynthesis, and inhibition.
Chembiochem: 2010, 11(1);35-45
[PubMed:19899094] [WorldCat.org] [DOI] (I p)