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⊟Summary[edit | edit source]
- pan ID?: SAUPAN001134000
- symbol?: tarJ
- synonym:
- description?: ribitol-5-phosphate dehydrogenase
- ribitol-5-phosphate dehydrogenase
- alcohol dehydrogenase, zinc-containing
- putative alcohol dehydrogenase, zinc-containing
- zinc-binding dehydrogenase
- xylitol dehydrogenase
- zinc (Zn2+)-binding dehydrogenase
- alcohol dehydrogenase
- alcohol dehydrogenase, zinc-dependent
- alcohol dehydrogenase GroES-like domain protein
- putative zinc-binding dehydrogenase
- ribitol-5-phosphate 2-dehydrogenase
- zinc-binding dehydrogenase family protein
- zinc-containing alcohol dehydrogenase
descriptions from strain specific annotations:
- strand?: +
- coordinates?: 1403475..1404527
- synteny block?: BlockID0006540
- occurrence?: in 100% of 34 strains
tarJ1 : ribulose-5-phosphate reductase 1 [1]
• A crystal structure is available : 6XH9
- Wall teichoic acid biosynthesis in Staphylococcus aureus is complicated by the presence of multiple overlapping pathways that are at least situationally functionally redundant. Initial steps involve modification of undecaprenyl phosphate with GlcNAc (TarO) and ManNAc (TarA) and then priming with two equivalents of glycerophosphate (TarB, TarF). This makes a starter unit for two types of WTA:
- TarL can prime and polymerize approximately 40 units of ribitol phosphate resulting into extended-length teichoic acids. The CDP-ribitol substrate is generated by the TarI1 and TarJ1 enzymes
- TarK can prime and polymerize approximately 20 units of ribitol phosphate resulting into short-length teichoic acids. The CDP-ribitol substrate is generated by the TarI2 and TarJ2 enzymes
- TarJ encodes ribulose-5-phosphate reductase (alt. ribitol-5-phosphate dehydrogenase) which reduces ribulose phosphate to ribitol phosphate
- TarI encodes ribitol-5-phosphate cytidylyltransferase which activates ribitol as CDP-ribitol
⊟Orthologs[edit | edit source]
04-02981:
SA2981_0256
08BA02176:
C248_0244
11819-97:
MS7_0246
6850:
RSAU_000200
71193:
ST398NM01_0269
ECT-R 2:
ECTR2_217
ED133:
SAOV_0195
ED98:
SAAV_0224
HO 5096 0412:
SAEMRSA15_02150
JH1:
SaurJH1_0247
JH9:
SaurJH9_0241
JKD6008:
SAA6008_00230
JKD6159:
SAA6159_00233
JSNZ:
JSNZ_000196
LGA251:
SARLGA251_02200 (tarJ)
M013:
M013TW_0237
MRSA252:
SAR0253
MSHR1132:
SAMSHR1132_02240
MSSA476:
SAS0233
Mu3:
SAHV_0255
Mu50:
SAV0256
MW2:
MW0232
RF122:
SAB0195
ST398:
SAPIG0269
T0131:
SAT0131_00249 (greA)
TCH60:
HMPREF0772_10246 (tarJ)
TW20:
SATW20_02580 (tarJ)
USA300_TCH1516:
USA300HOU_0267
VC40:
SAVC_01020
⊟Genome Viewer[edit | edit source]
| COL | |
| N315 | |
| NCTC8325 | |
| Newman | |
| USA300_FPR3757 |
⊟Alignments[edit | edit source]
- alignment of orthologues: CLUSTAL format alignment by MAFFT L-INS-i (v7.307)
COL MINQVYQLVAPRQFEVTYNNVDIYSDYVIVRPLYMSICAADQRYYTGSRDENVLSQKLPM
N315 MINQVYQLVAPRQFEVTYNNVDIYSDYVIVRPLYMSICAADQRYYTGSRDENVLSQKLPM
NCTC8325 MINQVYQLVAPRQFEVTYNNVDIYSDYVIVRPLYMSICAADQRYYTGSRDENVLSQKLPM
Newman MINQVYQLVAPRQFEVTYNNVDIYSDYVIVRPLYMSICAADQRYYTGSRDENVLSQKLPM
USA300_FPR3757 MINQVYQLVAPRQFEVTYNNVDIYSDYVIVRPLYMSICAADQRYYTGSRDENVLSQKLPM
************************************************************
COL SLIHEGVGEVVFDSKGVFNKGTKVVMVPNTPTEKDDVIAENYLKSSYFRSSGHDGFMQDF
N315 SLIHEGVGEVVFDSKGVFNKGTKVVMVPNTPTEKDDVIAENYLKSSYFRSSGHDGFMQDF
NCTC8325 SLIHEGVGEVVFDSKGVFNKGTKVVMVPNTPTEKDDVIAENYLKSSYFRSSGHDGFMQDF
Newman SLIHEGVGEVVFDSKGVFNKGTKVVMVPNTPTEKDDVIAENYLKSSYFRSSGHDGFMQDF
USA300_FPR3757 SLIHEGVGEVVFDSKGVFNKGTKVVMVPNTPTEKDDVIAENYLKSSYFRSSGHDGFMQDF
************************************************************
COL VLLNHDRAVPLPDDIDLSIISYTELVTVSLHAIRRFEKKSISNKNTFGIWGDGNLGYITA
N315 VLLNHDRAVPLPDDIDLSIISYTELVTVSLHAIRRFEKKSISNKNTFGIWGDGNLGYITA
NCTC8325 VLLNHDRAVPLPDDIDLSIISYTELVTVSLHAIRRFEKKSISNKNTFGIWGDGNLGYITA
Newman VLLNHDRAVPLPDDIDLSIISYTELVTVSLHAIRRFEKKSISNKNTFGIWGDGNLGYITA
USA300_FPR3757 VLLNHDRAVPLPDDIDLSIISYTELVTVSLHAIRRFEKKSISNKNTFGIWGDGNLGYITA
************************************************************
COL ILLRKLYPESKIYVFGKTDYKLSHFSFVDDVFFINKIPEGLTFDHAFECVGGRGSQSAIN
N315 ILLRKLYPESKIYVFGKTDYKLSHFSFVDDVFFINKIPEGLTFDHAFECVGGRGSQSAIN
NCTC8325 ILLRKLYPESKIYVFGKTDYKLSHFSFVDDVFFINKIPEGLTFDHAFECVGGRGSQSAIN
Newman ILLRKLYPESKIYVFGKTDYKLSHFSFVDDVFFINKIPEGLTFDHAFECVGGRGSQSAIN
USA300_FPR3757 ILLRKLYPESKIYVFGKTDYKLSHFSFVDDVFFINKIPEGLTFDHAFECVGGRGSQSAIN
************************************************************
COL QMIDYISPEGSIALLGVSEFPVEVNTRLVLEKGLTLIGSSRSGSKDFQDVVDLYIQYPDI
N315 QMIDYISPEGSIALLGVSEFPVEVNTRLVLEKGLTLIGSSRSGSKDFQDVVDLYIQYPDI
NCTC8325 QMIDYISPEGSIALLGVSEFPVEVNTRLVLEKGLTLIGSSRSGSKDFQDVVDLYIQYPDI
Newman QMIDYISPEGSIALLGVSEFPVEVNTRLVLEKGLTLIGSSRSGSKDFQDVVDLYIQYPDI
USA300_FPR3757 QMIDYISPEGSIALLGVSEFPVEVNTRLVLEKGLTLIGSSRSGSKDFQDVVDLYIQYPDI
************************************************************
COL VDKLALLKGQEFEIATINDLTEAFEADLSTSWGKTVLKWIM
N315 VDKLALLKGQEYEIATINDLTEAFEADLSTSWGKTVLKWIM
NCTC8325 VDKLALLKGQEFEIATINDLTEAFEADLSTSWGKTVLKWIM
Newman VDKLALLKGQEFEIATINDLTEAFEADLSTSWGKTVLKWIM
USA300_FPR3757 VDKLALLKGQEFEIATINDLTEAFEADLSTSWGKTVLKWIM
***********:*****************************
- ↑ Stephanie Brown, Yu-Hui Zhang, Suzanne Walker
A revised pathway proposed for Staphylococcus aureus wall teichoic acid biosynthesis based on in vitro reconstitution of the intracellular steps.
Chem Biol: 2008, 15(1);12-21
[PubMed:18215769] [WorldCat.org] [DOI] (P p)Timothy C Meredith, Jonathan G Swoboda, Suzanne Walker
Late-stage polyribitol phosphate wall teichoic acid biosynthesis in Staphylococcus aureus.
J Bacteriol: 2008, 190(8);3046-56
[PubMed:18281399] [WorldCat.org] [DOI] (I p)Jonathan G Swoboda, Jennifer Campbell, Timothy C Meredith, Suzanne Walker
Wall teichoic acid function, biosynthesis, and inhibition.
Chembiochem: 2010, 11(1);35-45
[PubMed:19899094] [WorldCat.org] [DOI] (I p)