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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01663
  • pan locus tag?: SAUPAN004143000
  • symbol: SAOUHSC_01663
  • pan gene symbol?: dnaG
  • synonym:
  • product: DNA primase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_01663
  • symbol: SAOUHSC_01663
  • product: DNA primase
  • replicon: chromosome
  • strand: -
  • coordinates: 1575828..1577627
  • length: 1800
  • essential: yes [1] DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    TTGCGAATAGATCAATCGATCATTAATGAAATAAAAGATAAAACCGACATTTTAGACTTG
    GTAAGTGAATATGTAAAATTAGAAAAGAGAGGACGCAATTATATAGGTTTGTGTCCTTTT
    CATGATGAAAAGACACCTTCATTTACAGTTTCTGAAGATAAACAAATTTGTCATTGTTTT
    GGTTGTAAAAAAGGTGGCAATGTTTTTCAATTTACTCAAGAAATTAAAGACATATCATTT
    GTTGAAGCGGTTAAAGAATTAGGTGATAGAGTTAATGTTGCTGTAGATATTGAGGCAACA
    CAATCTAACTCAAATGTTCAAATTGCTTCTGATGATTTACAAATGATTGAAATGCATGAG
    TTAATACAAGAATTTTATTATTACGCTTTAACAAAGACAGTCGAAGGCGAACAAGCATTA
    ACGTACTTACAAGAACGTGGTTTTACAGATGCGCTTATTAAAGAGCGAGGCATTGGCTTT
    GCACCCGATAGCTCACATTTTTGTCATGATTTTCTTCAAAAAAAGGGTTACGATATTGAA
    TTAGCATATGAAGCCGGATTATTATCACGTAACGAAGAAAATTTCAGTTATTACGATAGA
    TTTCGAAATCGTATTATGTTTCCTTTGAAAAATGCGCAAGGAAGAATTGTTGGATATTCA
    GGTCGAACATATACCGGTCAAGAACCAAAATACTTAAATAGTCCTGAAACACCTATCTTT
    CAAAAAAGAAAGTTGTTATACAACTTAGATAAAGCGCGTAAATCAATTAGAAAATTAGAT
    GAAATCGTATTACTAGAAGGTTTTATGGATGTTATAAAATCTGATACTGCTGGCTTGAAA
    AACGTTGTTGCAACAATGGGTACACAGTTGTCAGATGAACATATTACTTTTATACGAAAG
    TTAACATCAAATATAACATTAATGTTTGATGGGGATTTTGCGGGTAGTGAAGCAACACTT
    AAAACAGGTCAAAATTTGTTACAGCAAGGGCTAAATGTATTTGTTATACAATTGCCATCA
    GGCATGGATCCGGATGAATACATTGGTAAGTATGGCAACGATGCATTTACTGCTTTTGTA
    AAAAATGACAAAAAGTCATTTGCACATTATAAAGTGAGTATATTAAAAGATGAAATTGCA
    CATAATGACCTTTCATATGAACGTTATTTGAAAGAACTAAGTCATGATATTTCGCTTATG
    AAATCATCGATTTTGCAACAAAAGGCTTTAAATGATGTTGCACCATTTTTCAATGTTAGT
    CCTGAGCAATTAGCTAACGAAATACAATTCAATCAAGCACCAGCCAATTATTATCCAGAA
    GATGAGTATGGCGGTTACATTGAACCTGAGCCAATTGGTATGGCACAATTTGACAATTTG
    AGCCGTCAAGAAAAAGCGGAGCGAGCATTTTTAAAACATTTAATGAGAGATAAAGATACA
    TTTTTAAATTATTATGAAAGTGTTGATAAGGATAACTTCACAAATCAGCATTTTAAATAT
    GTATTCGAAGTCTTACATGATTTTTATGCGGAAAATGATCAATATAATATCAGTGATGCT
    GTGCAGTATGTTAATTCAAATGAGTTGAGAGAAACACTAATTAGCTTAGAACAATATAAT
    TTGAATGACGAACCATATGAAAATGAAATTGATGATTATGTCAATGTTATTAATGAAAAA
    GGACAAGAAACAATTGAGTCATTGAATCATAAATTAAGGGAAGCTACAAGGATTGGCGAT
    GTAGAATTACAAAAATACTATTTACAGCAAATTGTTGCTAAGAATAAAGAACGCATGTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_01663
  • symbol: SAOUHSC_01663
  • description: DNA primase
  • length: 599
  • theoretical pI: 4.71665
  • theoretical MW: 69293.3
  • GRAVY: -0.571619

⊟Function[edit | edit source]

  • reaction:
    EC 2.7.7.-?  ExPASy
  • TIGRFAM:
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA primase (TIGR01391; EC 2.7.7.-; HMM-score: 509.6)
    and 4 more
    Unknown function General DNA primase-like protein (TIGR00646; HMM-score: 33.6)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair replicative DNA helicase (TIGR00665; EC 3.6.4.12; HMM-score: 21.1)
    Genetic information processing Transcription RNA processing ribonuclease M5 (TIGR00334; EC 3.1.26.8; HMM-score: 16.7)
    Genetic information processing Mobile and extrachromosomal element functions Prophage functions phage replicative helicase, DnaB family (TIGR03600; HMM-score: 14.2)
  • TheSEED  :
    • DNA primase (EC 2.7.7.-)
    DNA Metabolism DNA replication DNA-replication  DNA primase (EC 2.7.7.-)
  • PFAM:
    no clan defined Toprim_N; DNA primase catalytic core, N-terminal domain (PF08275; HMM-score: 139.3)
    Zn_Beta_Ribbon (CL0167) zf-CHC2; CHC2 zinc finger (PF01807; HMM-score: 130)
    and 5 more
    Toprim-like (CL0413) Toprim_4; Toprim domain (PF13662; HMM-score: 53.5)
    Toprim_2; Toprim-like (PF13155; HMM-score: 52.9)
    no clan defined DnaB; DnaB-like helicase N terminal domain (PF00772; HMM-score: 26.6)
    Toprim-like (CL0413) Toprim_3; Toprim domain (PF13362; HMM-score: 26.5)
    Toprim; Toprim domain (PF01751; HMM-score: 16.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Mg2+, Zn2+
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.074085
    • TAT(Tat/SPI): 0.003323
    • LIPO(Sec/SPII): 0.00402
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MRIDQSIINEIKDKTDILDLVSEYVKLEKRGRNYIGLCPFHDEKTPSFTVSEDKQICHCFGCKKGGNVFQFTQEIKDISFVEAVKELGDRVNVAVDIEATQSNSNVQIASDDLQMIEMHELIQEFYYYALTKTVEGEQALTYLQERGFTDALIKERGIGFAPDSSHFCHDFLQKKGYDIELAYEAGLLSRNEENFSYYDRFRNRIMFPLKNAQGRIVGYSGRTYTGQEPKYLNSPETPIFQKRKLLYNLDKARKSIRKLDEIVLLEGFMDVIKSDTAGLKNVVATMGTQLSDEHITFIRKLTSNITLMFDGDFAGSEATLKTGQNLLQQGLNVFVIQLPSGMDPDEYIGKYGNDAFTAFVKNDKKSFAHYKVSILKDEIAHNDLSYERYLKELSHDISLMKSSILQQKALNDVAPFFNVSPEQLANEIQFNQAPANYYPEDEYGGYIEPEPIGMAQFDNLSRQEKAERAFLKHLMRDKDTFLNYYESVDKDNFTNQHFKYVFEVLHDFYAENDQYNISDAVQYVNSNELRETLISLEQYNLNDEPYENEIDDYVNVINEKGQETIESLNHKLREATRIGDVELQKYYLQQIVAKNKERM

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [2] [3]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Roy R Chaudhuri, Andrew G Allen, Paul J Owen, Gil Shalom, Karl Stone, Marcus Harrison, Timothy A Burgis, Michael Lockyer, Jorge Garcia-Lara, Simon J Foster, Stephen J Pleasance, Sarah E Peters, Duncan J Maskell, Ian G Charles
    Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH).
    BMC Genomics: 2009, 10;291
    [PubMed:19570206] [WorldCat.org] [DOI] (I e)
  2. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  4. ↑ 4.0 4.1 4.2 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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