PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_1522 [new locus tag: SAUSA300_RS08305 ]
• pan locus tag^?: SAUPAN004143000
• symbol: dnaG
• pan gene symbol^?: dnaG
• synonym:
• product: DNA primase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_1522 [new locus tag: SAUSA300_RS08305 ]
• symbol: dnaG
• product: DNA primase
• replicon: chromosome
• strand: -
• coordinates: 1672626..1674425
• length: 1800
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3914461 NCBI
• RefSeq: YP_494217 NCBI
• BioCyc: see SAUSA300_RS08305
• MicrobesOnline: 1293037 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

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  TTGCGAATAGATCAATCGATCATTAATGAAATAAAAGATAAAACCGACATTTTAGACTTG
  GTAAGTGAATATGTAAAATTAGAAAAGAGAGGACGCAATTATATAGGTTTGTGTCCTTTT
  CATGATGAAAAGACACCTTCATTTACAGTTTCTGAAGATAAACAAATTTGTCATTGTTTT
  GGTTGTAAAAAAGGTGGCAATGTTTTTCAATTTACTCAAGAAATTAAAGACATATCATTT
  GTTGAAGCGGTTAAAGAATTAGGTGATAGAGTTAATGTTGCTGTAGATATTGAGGCAACA
  CAATCTAACTCAAATGTTCAAATTGCTTCTGATGATTTACAAATGATTGAAATGCATGAG
  TTAATACAAGAATTTTATTATTACGCTTTAACAAAGACAGTCGAAGGCGAACAAGCATTA
  ACGTACTTACAAGAACGTGGTTTTACAGATGCGCTTATTAAAGAGCGAGGCATTGGCTTT
  GCACCCGATAGCTCACATTTTTGTCATGATTTTCTTCAAAAAAAGGGTTACGATATTGAA
  TTAGCATATGAAGCCGGATTATTATCACGTAACGAAGAAAATTTCAGTTATTACGATAGA
  TTTCGAAATCGTATTATGTTTCCTTTGAAAAATGCGCAAGGAAGAATTGTTGGATATTCA
  GGTCGAACATATACCGGTCAAGAACCAAAATACTTAAATAGTCCTGAAACACCTATCTTT
  CAAAAAAGAAAGTTGTTATACAACTTAGATAAAGCGCGTAAATCAATTAGAAAATTAGAT
  GAAATCGTATTACTAGAAGGTTTTATGGATGTTATAAAATCTGATACTGCTGGCTTGAAA
  AACGTTGTTGCAACAATGGGTACACAGTTGTCAGATGAACATATTACTTTTATACGAAAG
  TTAACATCAAATATAACATTAATGTTTGATGGGGATTTTGCGGGTAGTGAAGCAACACTT
  AAAACAGGTCAAAATTTGTTACAGCAAGGGCTAAATGTATTTGTTATACAATTGCCATCA
  GGCATGGATCCGGATGAATACATTGGTAAGTATGGCAACGATGCATTTACTGCTTTTGTA
  AAAAATGACAAAAAGTCATTTGCACATTATAAAGTGAGTATATTAAAAGATGAAATTGCA
  CATAATGACCTTTCATATGAACGTTATTTGAAAGAACTAAGTCATGATATTTCGCTTATG
  AAATCATCGATTTTGCAACAAAAGGCTTTAAATGATGTTGCACCATTTTTCAATGTTAGT
  CCTGAGCAATTAGCTAACGAAATACAATTCAATCAAGCACCAGCCAATTATTATCCAGAA
  GATGAGTATGGCGGTTACATTGAACCTGAGCCAATTGGTATGGCACAATTTGACAATTTG
  AGCCGTCAAGAAAAAGCGGAGCGAGCATTTTTAAAACATTTAATGAGAGATAAAGATACA
  TTTTTAAATTATTATGAAAGTGTTGATAAGGATAACTTCACAAATCAGCATTTTAAATAT
  GTATTCGAAGTCTTACATGATTTTTATGCGGAAAATGATCAATATAATATCAGTGATGCT
  GTGCAGTATGTTAATTCAAATGAGTTGAGAGAAACACTAATTAGCTTAGAACAATATAAT
  TTGAATGACGAACCATATGAAAATGAAATTGATGATTATGTCAATGTTATTAATGAAAAA
  GGACAAGAAACAATTGAGTCATTGAATCATAAATTAAGGGAAGCTACAAGGATTGGCGAT
  GTAGAATTACAAAAATACTATTTACAGCAAATTGTTGCTAAGAATAAAGAACGCATGTAG
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⊟Protein

⊟General

• locus tag: SAUSA300_1522 [new locus tag: SAUSA300_RS08305 ]
• symbol: DnaG
• description: DNA primase
• length: 599
• theoretical pI: 4.71665
• theoretical MW: 69293.3
• GRAVY: -0.571619

⊟Function

• reaction:
  EC 2.7.7.-?  ExPASy
• TIGRFAM:
  Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA primase (TIGR01391; EC 2.7.7.-; HMM-score: 509.6)
  and 4 more
  Unknown function General DNA primase-like protein (TIGR00646; HMM-score: 33.6)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair replicative DNA helicase (TIGR00665; EC 3.6.4.12; HMM-score: 21.1)
  Genetic information processing Transcription RNA processing ribonuclease M5 (TIGR00334; EC 3.1.26.8; HMM-score: 16.7)
  Genetic information processing Mobile and extrachromosomal element functions Prophage functions phage replicative helicase, DnaB family (TIGR03600; HMM-score: 14.2)
• TheSEED  :
  • DNA primase (EC 2.7.7.-)
  DNA Metabolism DNA replication DNA-replication  DNA primase (EC 2.7.7.-)
• PFAM:
  no clan defined Toprim_N; DNA primase catalytic core, N-terminal domain (PF08275; HMM-score: 139.3)
  Zn_Beta_Ribbon (CL0167) zf-CHC2; CHC2 zinc finger (PF01807; HMM-score: 130)
  and 5 more
  Toprim-like (CL0413) Toprim_4; Toprim domain (PF13662; HMM-score: 53.5)
  Toprim_2; Toprim-like (PF13155; HMM-score: 52.9)
  no clan defined DnaB; DnaB-like helicase N terminal domain (PF00772; HMM-score: 26.6)
  Toprim-like (CL0413) Toprim_3; Toprim domain (PF13362; HMM-score: 26.5)
  Toprim; Toprim domain (PF01751; HMM-score: 16.8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors: Mg²⁺, Zn²⁺
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.074085
  • TAT(Tat/SPI): 0.003323
  • LIPO(Sec/SPII): 0.00402
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87161696 NCBI
• RefSeq: YP_494217 NCBI
• UniProt: A0A0H2XHU2 UniProt

⊟Protein sequence

• MRIDQSIINEIKDKTDILDLVSEYVKLEKRGRNYIGLCPFHDEKTPSFTVSEDKQICHCFGCKKGGNVFQFTQEIKDISFVEAVKELGDRVNVAVDIEATQSNSNVQIASDDLQMIEMHELIQEFYYYALTKTVEGEQALTYLQERGFTDALIKERGIGFAPDSSHFCHDFLQKKGYDIELAYEAGLLSRNEENFSYYDRFRNRIMFPLKNAQGRIVGYSGRTYTGQEPKYLNSPETPIFQKRKLLYNLDKARKSIRKLDEIVLLEGFMDVIKSDTAGLKNVVATMGTQLSDEHITFIRKLTSNITLMFDGDFAGSEATLKTGQNLLQQGLNVFVIQLPSGMDPDEYIGKYGNDAFTAFVKNDKKSFAHYKVSILKDEIAHNDLSYERYLKELSHDISLMKSSILQQKALNDVAPFFNVSPEQLANEIQFNQAPANYYPEDEYGGYIEPEPIGMAQFDNLSRQEKAERAFLKHLMRDKDTFLNYYESVDKDNFTNQHFKYVFEVLHDFYAENDQYNISDAVQYVNSNELRETLISLEQYNLNDEPYENEIDDYVNVINEKGQETIESLNHKLREATRIGDVELQKYYLQQIVAKNKERM

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications