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NCBI: 03-AUG-2016
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus NCTC8325
- locus tag: SAOUHSC_01099
- pan locus tag?: SAUPAN003382000
- symbol: SAOUHSC_01099
- pan gene symbol?: mutS2
- synonym:
- product: recombination and DNA strand exchange inhibitor protein
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAOUHSC_01099
- symbol: SAOUHSC_01099
- product: recombination and DNA strand exchange inhibitor protein
- replicon: chromosome
- strand: +
- coordinates: 1059406..1061754
- length: 2349
- essential: no DEG other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3920741 NCBI
- RefSeq: YP_499643 NCBI
- BioCyc: G1I0R-1032 BioCyc
- MicrobesOnline: 1289556 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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2341ATGAGACAAAAAACATTAGACGTCTTAGAATTTGAAAAAATAAAATCACTCGTTGCCAAT
GAAACTATTAGTGACTTAGGCTTGGAAAAGGTCAATCAAATGATGCCAGCTACTAATTTT
GAAACGGTTGTTTTTCAAATGGAAGAAACGGATGAGATTGCTCAAATCTATAATAAGCAT
CGTTTACCAAGCTTGAGTGGCTTATCTAAAGTATCAGCATTCATTCATCGCGCTGATATT
GGCGGCGTTTTAAATGTATCAGAGCTTAACTTGATAAAAAGATTAATTCAAGTACAAAAT
CAATTCAAGACATTTTATAATCAATTGGTTGAAGAAGATGAAGGTGTTAAATACCCAATA
TTAGATGACAAGATGAATCAATTACCTGTGTTAACTGATCTTTTTCAACAAATAAATGAA
ACATGCGATACGTATGATTTATATGATAATGCGAGTTATGAATTGCAAGGGATTAGAAGT
AAAATTTCTAGCACGAATCAACGTATTAGACAAAATTTGGACCGTATTGTTAAAAGCCAA
GCAAATCAGAAAAAATTATCAGATGCTATTGTAACAGTTAGGAATGAAAGAAACGTTATA
CCTGTCAAAGCTGAATATCGACAAGATTTTAATGGGATTGTACATGATCAATCTGCTTCA
GGACAAACATTGTATATTGAGCCATCATCAGTTGTTGAAATGAATAATCAAATTAGTCGA
TTACGTCATGACGAAGCAATTGAAAAAGAACGCATTTTAACGCAACTAACTGGTTATGTG
GCTGCGGACAAAGATGCACTACTTGTGGCAGAACAAGTCATGGGTCAGTTAGATTTTTTA
ATCGCAAAAGCGAGATATAGTAGAAGTATTAAAGGAACAAAGCCGATATTTAAAGAGGAC
CGTACTGTATATTTACCTAAAGCATACCATCCATTATTAAATCGTGAGACTGTTGTAGCT
AATACCATCGAATTTATGGAAGATATTGAAACGGTAATTATTACAGGACCGAATACAGGT
GGTAAAACTGTAACATTAAAAACATTAGGTTTAATTATTGTTATGGCTCAATCAGGATTG
TTGATTCCCACACTTGATGGTAGTCAGTTAAGTGTATTTAAAAATGTATATTGCGATATC
GGAGATGAACAATCAATAGAACAATCATTATCAACTTTTTCATCTCATATGACGAATATA
GTTGAAATTTTAAAGCATGCAGACAAACATAGTTTAGTTTTATTTGATGAATTAGGTGCA
GGTACAGATCCGAGTGAAGGTGCTGCATTAGCAATGAGCATTTTAGATCATGTTAGAAAA
ATTGGTTCTCTAGTAATGGCAACGACGCACTATCCTGAACTTAAAGCATATAGTTATAAT
CGAGAAGGCGTTATGAATGCGAGTGTAGAATTTGATGTAGATACTTTGAGTCCAACGTAT
AAGTTATTAATGGGTGTGCCGGGTCGTTCAAATGCTTTTGACATTTCTAAAAAGTTAGGT
CTTAGTTTGAATATTATTAATAAGGCTAAGACGATGATTGGTACTGATGAAAAAGAAATA
AATGAAATGATTGAATCATTAGAGCGTAATTACAAACGTGTAGAGACACAGAGGTTAGAA
CTGGACCGTCTTGTAAAAGAAGCGGAGCAAGTGCATGATGATTTATCTAAGCAGTATCAA
CAATTCCAAAATTATGAAAAGTCTCTAATAGAGGAAGCGAAAGAAAAAGCAAATCAGAAG
ATTAAGGCTGCAACAAAAGAAGCTGACGATATTATTAAAGACTTAAGACAATTGCGTGAA
CAAAAAGGTGCAGATGTTAAAGAACATGAATTGATTGATAAGAAGAAACGATTAGATGAT
CATTATGAAGCGAAATCTATAAAGCAAAATGTACAAAAGCAAAAATACGATAAAATTGTT
GCTGGTGATGAAGTAAAAGTATTATCTTACGGTCAAAAGGGTGAAGTTTTAGAAATTGTC
AATGATGAAGAAGCAATTGTTCAAATGGGAATTATTAAAATGAAGTTACCTATTGAAGAT
TTAGAGAAAAAACAAAAAGAAAAAGTTAAGCCAACGAAAATGGTTACACGTCAAAATCGT
CAAACAATTAAAACTGAACTTGACTTACGAGGCTATCGTTATGAGGATGCTTTAATTGAA
CTAGATCAATATTTAGATCAAGCCGTTTTAAGTAATTACGAACAAGTTTATATCATTCAT
GGTAAAGGTACAGGTGCACTTCAAAAAGGTGTACAACAACATTTGAAAAAGCATAAAAGT
GTTAGTGACTTTAGAGGTGGTATGCCAAGCGAAGGTGGATTTGGCGTTACCGTTGCAACA
CTAAAATAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAOUHSC_01099
- symbol: SAOUHSC_01099
- description: recombination and DNA strand exchange inhibitor protein
- length: 782
- theoretical pI: 6.14125
- theoretical MW: 88660.6
- GRAVY: -0.469437
⊟Function[edit | edit source]
- reaction: EC 3.1.-.-? ExPASy
- TIGRFAM: DNA metabolism Other MutS2 family protein (TIGR01069; HMM-score: 784.5)and 4 moreDNA metabolism DNA replication, recombination, and repair DNA mismatch repair protein MutS (TIGR01070; HMM-score: 139.8)Protein fate Protein and peptide secretion and trafficking heme ABC exporter, ATP-binding protein CcmA (TIGR01189; EC 3.6.3.41; HMM-score: 13.2)Transport and binding proteins Other heme ABC exporter, ATP-binding protein CcmA (TIGR01189; EC 3.6.3.41; HMM-score: 13.2)Cellular processes Pathogenesis virulence factor Mce family protein (TIGR00996; HMM-score: 12.5)
- TheSEED :
- Recombination inhibitory protein MutS2
- PFAM: P-loop_NTPase (CL0023) MutS_V; MutS domain V (PF00488; HMM-score: 122.9)and 11 moreno clan defined Smr; Smr domain (PF01713; HMM-score: 68.7)DUF2935; Domain of unknown function (DUF2935) (PF11155; HMM-score: 15.9)P-loop_NTPase (CL0023) AAA_21; AAA domain, putative AbiEii toxin, Type IV TA system (PF13304; HMM-score: 13.6)no clan defined Spc7; Spc7 kinetochore protein (PF08317; HMM-score: 13.3)PilJ; Type IV pili methyl-accepting chemotaxis transducer N-term (PF13675; HMM-score: 11.1)YtxH; YtxH-like protein (PF12732; HMM-score: 10)IncA; IncA protein (PF04156; HMM-score: 9.5)DUF2203; Uncharacterized conserved protein (DUF2203) (PF09969; HMM-score: 9.5)VSG_B; Trypanosomal VSG domain (PF13206; HMM-score: 8)YjbJ-CsbD-like (CL0406) DUF883; Bacterial protein of unknown function (DUF883) (PF05957; HMM-score: 7.6)no clan defined BRE1; BRE1 E3 ubiquitin ligase (PF08647; HMM-score: 7.6)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: -1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.004035
- TAT(Tat/SPI): 0.000275
- LIPO(Sec/SPII): 0.000445
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MRQKTLDVLEFEKIKSLVANETISDLGLEKVNQMMPATNFETVVFQMEETDEIAQIYNKHRLPSLSGLSKVSAFIHRADIGGVLNVSELNLIKRLIQVQNQFKTFYNQLVEEDEGVKYPILDDKMNQLPVLTDLFQQINETCDTYDLYDNASYELQGIRSKISSTNQRIRQNLDRIVKSQANQKKLSDAIVTVRNERNVIPVKAEYRQDFNGIVHDQSASGQTLYIEPSSVVEMNNQISRLRHDEAIEKERILTQLTGYVAADKDALLVAEQVMGQLDFLIAKARYSRSIKGTKPIFKEDRTVYLPKAYHPLLNRETVVANTIEFMEDIETVIITGPNTGGKTVTLKTLGLIIVMAQSGLLIPTLDGSQLSVFKNVYCDIGDEQSIEQSLSTFSSHMTNIVEILKHADKHSLVLFDELGAGTDPSEGAALAMSILDHVRKIGSLVMATTHYPELKAYSYNREGVMNASVEFDVDTLSPTYKLLMGVPGRSNAFDISKKLGLSLNIINKAKTMIGTDEKEINEMIESLERNYKRVETQRLELDRLVKEAEQVHDDLSKQYQQFQNYEKSLIEEAKEKANQKIKAATKEADDIIKDLRQLREQKGADVKEHELIDKKKRLDDHYEAKSIKQNVQKQKYDKIVAGDEVKVLSYGQKGEVLEIVNDEEAIVQMGIIKMKLPIEDLEKKQKEKVKPTKMVTRQNRQTIKTELDLRGYRYEDALIELDQYLDQAVLSNYEQVYIIHGKGTGALQKGVQQHLKKHKSVSDFRGGMPSEGGFGVTVATLK
⊟Experimental data[edit | edit source]
- experimentally validated: PeptideAtlas [1] [2]
- protein localization: data available for COL
- quantitative data / protein copy number per cell:
- interaction partners:
SAOUHSC_02117 (gatA) aspartyl/glutamyl-tRNA amidotransferase subunit A [3] (data from MRSA252) SAOUHSC_02116 (gatB) aspartyl/glutamyl-tRNA amidotransferase subunit B [3] (data from MRSA252) SAOUHSC_00519 (rplA) 50S ribosomal protein L1 [3] (data from MRSA252) SAOUHSC_02507 (rplV) 50S ribosomal protein L22 [3] (data from MRSA252) SAOUHSC_02510 (rplW) 50S ribosomal protein L23 [3] (data from MRSA252) SAOUHSC_01232 (rpsB) 30S ribosomal protein S2 [3] (data from MRSA252) SAOUHSC_02506 (rpsC) 30S ribosomal protein S3 [3] (data from MRSA252) SAOUHSC_02494 (rpsE) 30S ribosomal protein S5 [3] (data from MRSA252) SAOUHSC_00187 formate acetyltransferase [3] (data from MRSA252) SAOUHSC_00530 elongation factor Tu [3] (data from MRSA252) SAOUHSC_00679 hypothetical protein [3] (data from MRSA252) SAOUHSC_00878 hypothetical protein [3] (data from MRSA252) SAOUHSC_00906 hypothetical protein [3] (data from MRSA252) SAOUHSC_01040 pyruvate dehydrogenase complex, E1 component subunit alpha [3] (data from MRSA252) SAOUHSC_01150 cell division protein FtsZ [3] (data from MRSA252) SAOUHSC_01416 dihydrolipoamide succinyltransferase [3] (data from MRSA252) SAOUHSC_01794 glyceraldehyde 3-phosphate dehydrogenase 2 [3] (data from MRSA252) SAOUHSC_01801 isocitrate dehydrogenase [3] (data from MRSA252) SAOUHSC_01806 pyruvate kinase [3] (data from MRSA252) SAOUHSC_01819 hypothetical protein [3] (data from MRSA252) SAOUHSC_01909 S-adenosylmethionine synthetase [3] (data from MRSA252) SAOUHSC_02365 UDP-N-acetylglucosamine 1-carboxyvinyltransferase [3] (data from MRSA252)
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: SAOUHSC_01096 > SAOUHSC_01097 > SAOUHSC_01098 > SAOUHSC_01099predicted SigA promoter [4] : S452 > SAOUHSC_01096 > SAOUHSC_01097 > SAOUHSC_01098 > SAOUHSC_01099 > S453 > SAOUHSC_01100 > S454 > uvrC
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: [4]
Multi-gene expression profiles
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
Proteomics: 2015, 15(21);3648-61
[PubMed:26224020] [WorldCat.org] [DOI] (I p) - ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
Sci Rep: 2017, 7(1);9718
[PubMed:28887440] [WorldCat.org] [DOI] (I e) - ↑ 3.00 3.01 3.02 3.03 3.04 3.05 3.06 3.07 3.08 3.09 3.10 3.11 3.12 3.13 3.14 3.15 3.16 3.17 3.18 3.19 3.20 3.21 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
J Proteome Res: 2011, 10(3);1139-50
[PubMed:21166474] [WorldCat.org] [DOI] (I p) - ↑ 4.0 4.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
PLoS Genet: 2016, 12(4);e1005962
[PubMed:27035918] [WorldCat.org] [DOI] (I e)