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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0991 [new locus tag: SA_RS05615 ]
  • pan locus tag?: SAUPAN003382000
  • symbol: mutS2
  • pan gene symbol?: mutS2
  • synonym:
  • product: recombination and DNA strand exchange inhibitor protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA0991 [new locus tag: SA_RS05615 ]
  • symbol: mutS2
  • product: recombination and DNA strand exchange inhibitor protein
  • replicon: chromosome
  • strand: +
  • coordinates: 1122096..1124444
  • length: 2349
  • essential: yes [1] DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGAGACAAAAAACATTAGACGTCTTAGAATTTGAAAAAATAAAATCACTCGTTGCCAAT
    GAAACTATTAGTGACTTAGGCTTGGAAAAGGTCAATCAAATGATGCCAGCTACTAATTTT
    GAAACGGTTGTTTTTCAAATGGAAGAAACGGATGAGATTGCTCAAATCTATAATAAGCAT
    CGTTTACCAAGCTTGAGTGGCTTATCTAAAGTATCAGCATTCATTCATCGCGCTGATATT
    GGCGGCGTTTTAAATGTATCAGAGCTTAACTTGATAAAAAGATTAATTCAAGTACAAAAT
    CAATTTAAGACATTTTATAATCAATTGGTTGAAGAAGATGAAGGTGTTAAATACCCAATA
    TTAGATGACAAGATGAATCAATTACCTGTGTTAACTGATCTTTTTCATCAAATAAATGAA
    ACATGCGATACGTATGATTTATATGATAATGCGAGTTATGAATTGCAAGGGATTAGAAGT
    AAAATTTCTAGCACGAATCAACGTATTAGACAAAATTTGGACCGTATTGTTAAAAGCCAA
    GCAAATCAGAAAAAATTATCAGATGCTATTGTAACAGTTAGGAATGAAAGAAACGTTATA
    CCTGTCAAAGCTGAATATCGACAAGATTTTAATGGGATTGTACATGATCAATCTGCTTCA
    GGACAAACATTGTATATTGAGCCATCATCAGTTGTTGAAATGAATAATCAAATTAGTCGA
    TTACGTCATGACGAAGCAATTGAAAAAGAACGCGTTTTAACGCAACTAACTGGTTATGTG
    GCTGCGGACAAAGATGCACTACTTGTGGCAGAACAAGTCATGGGGCAGTTAGATTTTTTA
    ATCGCAAAAGCGAGATATAGTAGAAGTATTAAAGGAACAAAGCCGATATTTAAAGAGGAA
    CGTACTGTATATTTACCTAAAGCATACCATCCATTATTAAATCGTGAGACTGTTGTAGCT
    AATACCATCGAATTTATGGAAGATATTGAAACGGTAATTATTACAGGACCGAATACAGGT
    GGTAAAACTGTAACATTAAAAACATTAGGTTTAATTATTGTTATGGCTCAATCAGGATTG
    TTGATTCCCACACTTGATGGTAGTCAGTTAAGTGTATTTAAAAATGTATATTGCGATATC
    GGAGATGAACAATCAATAGAACAATCATTATCAACTTTTTCATCTCATATGACGAATATA
    GTTGAAATTTTAAAGCATGCAGACAAACATAGTTTAGTTTTATTTGATGAATTAGGTGCA
    GGTACAGATCCAAGTGAAGGTGCTGCATTAGCAATGAGCATTTTAGATCATGTTAGAAAA
    ATTGGTTCTCTAGTAATGGCAACGACGCACTATCCTGAACTTAAAGCATATAGTTATAAT
    CGAGAAGGCGTTATGAATGCGAGTGTAGAATTTGATGTAGATACTTTGAGTCCAACGTAT
    AAGTTATTAATGGGTGTGCCGGGTCGTTCAAATGCTTTTGACATTTCTAAAAAGTTAGGT
    CTTAGTTTGAATATTATTAATAAGGCTAAGACGATGATTGGTACTGATGAAAAAGAAATA
    AATGAAATGATTGAATCATTAGAGCGTAATTATAAACGTGTAGAGACACAGAGGTTAGAA
    CTGGACCGTCTTGTAAAAGAAGCGGAGCAAGTGCATGATGATTTATCTAAGCAGTATCAA
    CAATTCCAAAATTATGAAAAGTCTCTAATAGAGGAAGCGAAAGAAAAAGCAAATCAGAAG
    ATTAAAGCTGCAACAAAAGAAGCTGACGATATTATTAAAGACTTAAGACAATTGCGTGAA
    CAAAAAGGTGCAGATGTTAAAGAACATGAATTGATTGATAAGAAGAAACGATTAGATGAT
    CATTATGAAGCGAAATCTATAAAGCAAAATGTACAAAAGCAAAAATACGATAAAATTGTT
    GCTGGTGATGAAGTAAAAGTATTATCTTACGGTCAAAAGGGTGAAGTTTTAGAAATTGTC
    AATGATGAAGAAGCAATTGTTCAAATGGGAATTATTAAAATGAAGTTACCTATTGAAGAT
    TTAGAGAAAAAACAAAAAGAAAAAGTTAAGCCAACGAAAATGGTTACACGTCAAAATCGT
    CAAACAATTAAAACTGAACTTGACTTACGAGGCTATCGTTATGAGGATGCTTTAATTGAA
    CTAGATCAATATTTAGATCAAGCCGTTTTAAGTAATTACGAACAAGTTTATATCATTCAT
    GGTAAAGGTACAGGTGCACTTCAAAAAGGTGTACAACAACATTTGAAGAAGCATAAAAGT
    GTTAGTGACTTTAGAGGTGGTATGCCAAGCGAAGGTGGATTTGGCGTTACCGTTGCAACA
    CTAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2349

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA0991 [new locus tag: SA_RS05615 ]
  • symbol: MutS2
  • description: recombination and DNA strand exchange inhibitor protein
  • length: 782
  • theoretical pI: 6.20805
  • theoretical MW: 88669.6
  • GRAVY: -0.469437

⊟Function[edit | edit source]

  • reaction:
    EC 3.1.-.-?  ExPASy
  • TIGRFAM:
    Genetic information processing DNA metabolism Other MutS2 family protein (TIGR01069; HMM-score: 782.3)
    and 4 more
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA mismatch repair protein MutS (TIGR01070; HMM-score: 139.4)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking heme ABC exporter, ATP-binding protein CcmA (TIGR01189; EC 3.6.3.41; HMM-score: 13.2)
    Metabolism Transport and binding proteins Other heme ABC exporter, ATP-binding protein CcmA (TIGR01189; EC 3.6.3.41; HMM-score: 13.2)
    Cellular processes Cellular processes Pathogenesis virulence factor Mce family protein (TIGR00996; HMM-score: 12.2)
  • TheSEED  :
    • Recombination inhibitory protein MutS2
    DNA Metabolism DNA repair DNA repair, bacterial MutL-MutS system  Recombination inhibitory protein MutS2
  • PFAM:
    P-loop_NTPase (CL0023) MutS_V; MutS domain V (PF00488; HMM-score: 122.7)
    and 11 more
    no clan defined Smr; Smr domain (PF01713; HMM-score: 68.7)
    DUF2935; Domain of unknown function (DUF2935) (PF11155; HMM-score: 16.3)
    P-loop_NTPase (CL0023) AAA_21; AAA domain, putative AbiEii toxin, Type IV TA system (PF13304; HMM-score: 13.6)
    no clan defined Spc7; Spc7 kinetochore protein (PF08317; HMM-score: 13.1)
    PilJ; Type IV pili methyl-accepting chemotaxis transducer N-term (PF13675; HMM-score: 11.2)
    DUF2203; Uncharacterized conserved protein (DUF2203) (PF09969; HMM-score: 9.6)
    YtxH; YtxH-like protein (PF12732; HMM-score: 9.5)
    IncA; IncA protein (PF04156; HMM-score: 9.4)
    VSG_B; Trypanosomal VSG domain (PF13206; HMM-score: 8)
    YjbJ-CsbD-like (CL0406) DUF883; Bacterial protein of unknown function (DUF883) (PF05957; HMM-score: 7.8)
    no clan defined BRE1; BRE1 E3 ubiquitin ligase (PF08647; HMM-score: 7.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004035
    • TAT(Tat/SPI): 0.000275
    • LIPO(Sec/SPII): 0.000445
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MRQKTLDVLEFEKIKSLVANETISDLGLEKVNQMMPATNFETVVFQMEETDEIAQIYNKHRLPSLSGLSKVSAFIHRADIGGVLNVSELNLIKRLIQVQNQFKTFYNQLVEEDEGVKYPILDDKMNQLPVLTDLFHQINETCDTYDLYDNASYELQGIRSKISSTNQRIRQNLDRIVKSQANQKKLSDAIVTVRNERNVIPVKAEYRQDFNGIVHDQSASGQTLYIEPSSVVEMNNQISRLRHDEAIEKERVLTQLTGYVAADKDALLVAEQVMGQLDFLIAKARYSRSIKGTKPIFKEERTVYLPKAYHPLLNRETVVANTIEFMEDIETVIITGPNTGGKTVTLKTLGLIIVMAQSGLLIPTLDGSQLSVFKNVYCDIGDEQSIEQSLSTFSSHMTNIVEILKHADKHSLVLFDELGAGTDPSEGAALAMSILDHVRKIGSLVMATTHYPELKAYSYNREGVMNASVEFDVDTLSPTYKLLMGVPGRSNAFDISKKLGLSLNIINKAKTMIGTDEKEINEMIESLERNYKRVETQRLELDRLVKEAEQVHDDLSKQYQQFQNYEKSLIEEAKEKANQKIKAATKEADDIIKDLRQLREQKGADVKEHELIDKKKRLDDHYEAKSIKQNVQKQKYDKIVAGDEVKVLSYGQKGEVLEIVNDEEAIVQMGIIKMKLPIEDLEKKQKEKVKPTKMVTRQNRQTIKTELDLRGYRYEDALIELDQYLDQAVLSNYEQVYIIHGKGTGALQKGVQQHLKKHKSVSDFRGGMPSEGGFGVTVATLK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:
    SA1517(citC)isocitrate dehydrogenase  [2] (data from MRSA252)
    SA1029(ftsZ)cell division protein FtsZ  [2] (data from MRSA252)
    SA1510(gapB)glyceraldehyde 3-phosphate dehydrogenase 2  [2] (data from MRSA252)
    SA1716(gatA)aspartyl/glutamyl-tRNA amidotransferase subunit A  [2] (data from MRSA252)
    SA1715(gatB)aspartyl/glutamyl-tRNA amidotransferase subunit B  [2] (data from MRSA252)
    SA1608(metK)S-adenosylmethionine synthetase  [2] (data from MRSA252)
    SA1926(murZ)UDP-N-acetylglucosamine 1-carboxyvinyltransferase  [2] (data from MRSA252)
    SA1244(odhB)dihydrolipoamide succinyltransferase  [2] (data from MRSA252)
    SA0943-1(pdhA)pyruvate dehydrogenase E1 component subunit alpha  [2] (data from MRSA252)
    SA0218(pflB)formate acetyltransferase  [2] (data from MRSA252)
    SA1520(pykA)pyruvate kinase  [2] (data from MRSA252)
    SA0496(rplA)50S ribosomal protein L1  [2] (data from MRSA252)
    SA2042(rplV)50S ribosomal protein L22  [2] (data from MRSA252)
    SA2045(rplW)50S ribosomal protein L23  [2] (data from MRSA252)
    SA1099(rpsB)30S ribosomal protein S2  [2] (data from MRSA252)
    SA2041(rpsC)30S ribosomal protein S3  [2] (data from MRSA252)
    SA2031(rpsE)30S ribosomal protein S5  [2] (data from MRSA252)
    SA0506(tuf)elongation factor Tu  [2] (data from MRSA252)
    SA0627hypothetical protein  [2] (data from MRSA252)
    SA0802hypothetical protein  [2] (data from MRSA252)
    SA0829hypothetical protein  [2] (data from MRSA252)
    SA1532hypothetical protein  [2] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ R Allyn Forsyth, Robert J Haselbeck, Kari L Ohlsen, Robert T Yamamoto, Howard Xu, John D Trawick, Daniel Wall, Liangsu Wang, Vickie Brown-Driver, Jamie M Froelich, Kedar G C, Paula King, Melissa McCarthy, Cheryl Malone, Brian Misiner, David Robbins, Zehui Tan, Zhan-yang Zhu Zy, Grant Carr, Deborah A Mosca, Carlos Zamudio, J Gordon Foulkes, Judith W Zyskind
    A genome-wide strategy for the identification of essential genes in Staphylococcus aureus.
    Mol Microbiol: 2002, 43(6);1387-400
    [PubMed:11952893] [WorldCat.org] [DOI] (P p)
  2. ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 2.14 2.15 2.16 2.17 2.18 2.19 2.20 2.21 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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