PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus COL
• locus tag: SACOL_RS08545 [old locus tag: SACOL1675 ]
• pan locus tag^?: SAUPAN004218000
• symbol: SACOL_RS08545
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL_RS08545 [old locus tag: SACOL1675 ]
• symbol: SACOL_RS08545
• product: hypothetical protein
• replicon: chromosome
• strand: -
• coordinates: 1704671..1705339
• length: 669
• essential: unknown other strains

⊟Accession numbers

• Location: NC_002951 (1704671..1705339) NCBI
• BioCyc: SACOL_RS08545 BioCyc
• MicrobesOnline: see SACOL1675

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGATAGATCAACAAACAATTTATCAATACATACAAAATGGAAAAATAGAAGAAGCGTTA
  CAAGCATTGTTCGGAAATATCGAAGAAAATCCTACAATTATTGAAAATTATATTAATGCT
  GGTATCGTACTTGCTGATGCGAATGAGATTGAAAAGGCAGAGCGTTTTTTCCAAAAAGCT
  TTAACAATAGATCCGAAGAATGGCGTCGTATTTTTTAATCTAGCAAATGTATATTATAAT
  CAGCAACGTTATCAAGAAGCTATTAAATTATATCAACAAGCATTACAAACAGAGATTGAA
  CAAGTTGATTGTAATTATATGATCGGTATGGCGTTTAATCAGTTAGAATCATTTAAGCTG
  GCATTGCCGTATTTAATGACTGCTGCGGAACTAGATAAAGACAAAGATGCAGAAGTTCAA
  TTTCAATATGGTCTTGTATTATGTCAATTAGAAATGTTTAATGAAGCCATAACTCAACTT
  AAACATGTATTAACGATTGATAAAAATCATGTTGATGCAAGATACAATTTGGGCTTAGCG
  TTATTTATGAAAAATGAAGATATTGATGAAGCAATAACTCATTTTAAAGAAGCTGTGACT
  ATCGACCCTAAACACTTATTAAGTCAGCATGCGCTGAAAACATTCACTAAAATGAAAGAG
  GAGGAGTAA
  60
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  669
  

⊟Protein

⊟General

• locus tag: SACOL_RS08545 [old locus tag: SACOL1675 ]
• symbol: SACOL_RS08545
• description: hypothetical protein
• length: 222
• theoretical pI: 4.41965
• theoretical MW: 25701.1
• GRAVY: -0.277477

⊟Function

• TIGRFAM:
  type IV pilus biogenesis/stability protein PilW (TIGR02521; HMM-score: 72.1)
  putative PEP-CTERM system TPR-repeat lipoprotein (TIGR02917; HMM-score: 70.8)
  and 8 more
  type III secretion low calcium response chaperone LcrH/SycD (TIGR02552; HMM-score: 56.5)
  Metabolism Transport and binding proteins Amino acids, peptides and amines mitochondrial precursor proteins import receptor (TIGR00990; HMM-score: 49.8)
  tol-pal system protein YbgF (TIGR02795; HMM-score: 31.4)
  putative peptide modification system cyclase (TIGR04510; HMM-score: 26.2)
  Unknown function General heme biosynthesis-associated TPR protein (TIGR00540; HMM-score: 24.5)
  pentatricopeptide repeat domain (TIGR00756; HMM-score: 21.9)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Salvage of nucleosides and nucleotides adenine phosphoribosyltransferase (TIGR01090; EC 2.4.2.7; HMM-score: 21.4)
  poly-beta-1,6 N-acetyl-D-glucosamine export porin PgaA (TIGR03939; HMM-score: 20.7)
• TheSEED: see SACOL1675
• PFAM:
  TPR (CL0020) TPR_1; Tetratricopeptide repeat (PF00515; HMM-score: 100)
  TPR_2; Tetratricopeptide repeat (PF07719; HMM-score: 99.7)
  TPR_8; Tetratricopeptide repeat (PF13181; HMM-score: 93.7)
  TPR_11; TPR repeat (PF13414; HMM-score: 86.9)
  TPR_12; Tetratricopeptide repeat (PF13424; HMM-score: 80.2)
  and 23 more
  TPR_17; Tetratricopeptide repeat (PF13431; HMM-score: 64.5)
  TPR_19; Tetratricopeptide repeat (PF14559; HMM-score: 63.2)
  TPR_16; Tetratricopeptide repeat (PF13432; HMM-score: 59.9)
  TPR_14; Tetratricopeptide repeat (PF13428; HMM-score: 59.4)
  ANAPC3; Anaphase-promoting complex, cyclosome, subunit 3 (PF12895; HMM-score: 53.3)
  TPR_7; Tetratricopeptide repeat (PF13176; HMM-score: 52.4)
  TPR_10; Tetratricopeptide repeat (PF13374; HMM-score: 47.6)
  TPR_6; Tetratricopeptide repeat (PF13174; HMM-score: 42.5)
  TPR_20; Tetratricopeptide repeat (PF14561; HMM-score: 35.5)
  TOM20_plant; Plant specific mitochondrial import receptor subunit TOM20 (PF06552; HMM-score: 28.3)
  TPR_9; Tetratricopeptide repeat (PF13371; HMM-score: 28.1)
  TPR_3; Tetratricopeptide repeat (PF07720; HMM-score: 26.8)
  PPR; PPR repeat (PF01535; HMM-score: 24.8)
  TPR_15; Tetratricopeptide repeat (PF13429; HMM-score: 22.2)
  no clan defined MIT; MIT (microtubule interacting and transport) domain (PF04212; HMM-score: 21.5)
  TPR (CL0020) PPR_2; PPR repeat family (PF13041; HMM-score: 18)
  NARP1; NMDA receptor-regulated protein 1 (PF12569; HMM-score: 16.8)
  TPR_21; Tetratricopeptide repeat-like domain (PF09976; HMM-score: 16.4)
  TPR_4; Tetratricopeptide repeat (PF07721; HMM-score: 15.8)
  Hect (CL0552) HECT_2; HECT-like Ubiquitin-conjugating enzyme (E2)-binding (PF09814; HMM-score: 15.3)
  TPR (CL0020) Fis1_TPR_C; Fis1 C-terminal tetratricopeptide repeat (PF14853; HMM-score: 13)
  Clathrin; Region in Clathrin and VPS (PF00637; HMM-score: 12.1)
  Sel1; Sel1 repeat (PF08238; HMM-score: 11.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.004087
  • TAT(Tat/SPI): 0.000163
  • LIPO(Sec/SPII): 0.000383
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446489174 NCBI
• RefSeq: WP_000567028 NCBI
• UniProt: see SACOL1675

⊟Protein sequence

• MIDQQTIYQYIQNGKIEEALQALFGNIEENPTIIENYINAGIVLADANEIEKAERFFQKALTIDPKNGVVFFNLANVYYNQQRYQEAIKLYQQALQTEIEQVDCNYMIGMAFNQLESFKLALPYLMTAAELDKDKDAEVQFQYGLVLCQLEMFNEAITQLKHVLTIDKNHVDARYNLGLALFMKNEDIDEAITHFKEAVTIDPKHLLSQHALKTFTKMKEEE

⊟Experimental data

• experimentally validated: see SACOL1675
• protein localization: see SACOL1675
• quantitative data / protein copy number per cell: see SACOL1675
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications